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01.06.2012 | Experimental Research

Effects of darbepoetin-alpha in spinal cord ischemia-reperfusion injury in the rabbit

verfasst von: Erdal Resit Yilmaz, Hayri Kertmen, Habibullah Dolgun, Bora Gürer, Ahmet Metin Sanli, Mehmet Ali Kanat, Ata Türker Arikok, Seda Yilmaz Bahsi, Berrin Imge Ergüder, Zeki Sekerci

Erschienen in: Acta Neurochirurgica | Ausgabe 6/2012

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Abstract

Background

Darbepoetin-alpha (DA) is a novel erythropoiesis-stimulating agent developed for treating anemia. In animal models, recombinant human erythropoietin has been reported to be beneficial for neuroprotection. In this study, we determined whether DA would protect the spinal cord against ischemia-reperfusion injury in a rabbit model.

Methods

Forty rabbits were randomized into five groups of eight animals each: group 1 (sham), group 2 (ischemia), group 3 (vehicle), group 4 (30 mg/kg methylprednisolone), group 5 (30 μg/kg DA). Only laparotomy was performed in the sham group. In all the other groups, the spinal cord ischemia model was created by a 20-min occlusion of the aorta just caudal to renal artery with an aneurysm clip. The drugs were administered immediately after the clamp was removed. The animals were killed 24 h later. Spinal cord segments between L2 and L5 were harvested for analysis. Neurological evaluation was performed with the Tarlov scoring system just before the animals were killed. Level of tissue malondialdehyde was analyzed as a marker of lipid peroxidation and tissue caspase-3 activity as a marker of apoptosis. Also, histopathological evaluation of the tissues was performed.

Results

Both malondialdehyde and caspase-3 levels were significantly decreased by DA administration. Histopathological evaluation of the tissues also demonstrated decrease in neuronal degeneration and infiltration parameters after DA administration. In the DA group, neurological outcome scores were statistically significantly better compared with the ischemia and the vehicle groups.

Conclusions

Although further studies considering different dose regimens and time intervals are required, DA was shown to be at least as effective as methylprednisolone in spinal cord ischemia/reperfusion model.

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Titel: Effects of darbepoetin-alpha in spinal cord ischemia-reperfusion injury in the rabbit
verfasst von: Erdal Resit Yilmaz
Hayri Kertmen
Habibullah Dolgun
Bora Gürer
Ahmet Metin Sanli
Mehmet Ali Kanat
Ata Türker Arikok
Seda Yilmaz Bahsi
Berrin Imge Ergüder
Zeki Sekerci
Publikationsdatum: 01.06.2012
Verlag: Springer Vienna
Erschienen in: Acta Neurochirurgica / Ausgabe 6/2012
Print ISSN: 0001-6268
Elektronische ISSN: 0942-0940
DOI: https://doi.org/10.1007/s00701-012-1298-0

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