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Archives of Virology

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01.04.2010 | Original Article

Isolates of Citrus tristeza virus that overcome Poncirus trifoliata resistance comprise a novel strain

verfasst von: S. J. Harper, T. E. Dawson, M. N. Pearson

Erschienen in: Archives of Virology | Ausgabe 4/2010

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Abstract

The economically important rootstock species Poncirus trifoliata is resistant to most isolates of Citrus tristeza virus (CTV), but not to members of the CTV resistance-breaking (RB) strain presently found in New Zealand. In this study, five known and suspected RB isolates were separated from field mixtures, and their genomes were sequenced in full. It was found that the RB isolates are members of a single phylogenetically distinct clade with an average of 90.3% genomic nucleotide sequence identity to the closest extant isolate, T36. These isolates also show evidence of multiple recombination events throughout their evolutionary history, with T36, T30 and VT-like isolates, and with each other. Finally, the genomic sequences of these isolates show that several genes contain unique polymorphisms that may or may not be involved in overcoming resistance. These data will aid in the understanding of host–virus interactions, and the mechanism of resistance in P. trifoliata.

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Albiach-Marti MR, Mawassi M, Gowda S, Satanarayana T, Hilf ME, Shanker S, Almira EC, Vives MC, Lopez C, Guerri J, Flores R, Moreno P, Garnsey SM, Dawson WO (2000) Sequences of Citrus tristeza virus separated in time and space are essentially identical. J Virol 74:6856–6865CrossRefPubMed

Albiach-Marti MR, Grosser JW, Gowda S, Mawassi M, Satyanarayana T, Garnsey SM, Dawson WO (2004) Citrus tristeza virus replicates and forms infections virions in protoplasts of resistant citrus relatives. Mol Breed 14:117–128CrossRef

Bitters WP (1972) Reaction of some new citrus hybrids and citrus introductions to inoculations with tristeza virus in California. In: Price WC (ed) Proceedings of the 5th conference of the International Organisation of citrus virologists. University of Florida Press, Gainesville, pp 112–120

Boni MF, Posada D, Feldman MW (2007) An exact nonparametric method for inferring mosaic structure in sequence triplets. Genetics 176:1035–1047CrossRefPubMed

Borja M, Rubio T, Scholthof HB, Jackson AO (1999) Restoration of wild-type virus by double recombination of tombusvirus mutants with a host transgene. Mol Plant Microbe Interact 12:153–162CrossRefPubMed

Broadbent P, Brlansky RH, Indsto J (1996) Biological characterisation of Australian isolates of Citrus tristeza virus and separation of subisolates by single aphid transmissions. Plant Dis 80:329–333

Dawson TE, Mooney PA (2000) Evidence for trifoliate resistance breaking isolates of Citrus tristeza virus in New Zealand. In: Yokomi RK, Lee RF, Da Graca JV (eds) Proceedings of the 14th conference of the International Organisation of citrus virologists. IOCV, Riverside, pp 69–76

Everett P (1928) Variety and rootstock experiments at Tauranga, seasons 1926–27 and 1927–28. New Zealand J Agric 37:187–192

Fagoaga C, Lopez C, Moreno P, Navarro L, Flores R, Pena L (2005) Viral-like symptoms induced by the ectopic expression of the p23 Gene of Citrus tristeza virus are citrus specific and do not correlate with the pathogenicity of the virus strain. Mol Plant Microbe Interact 18:435–445CrossRefPubMed

10.

Folimonova SY, Folomonov AS, Satyanarayana T, Dawson T (2008) Citrus tristeza virus: survival at the edge of the movement continuum. J Virol 82:6546–6556CrossRefPubMed

11.

Garnsey SM, Barrett HC, Hutchison DJ (1987) Identification of Citrus tristeza virus resistance in citrus relatives and its potential applications. Phytophylactica 19:187–191

12.

Ghorbel R, Lopez C, Fagoaga C, Moreno P, Flores R, Pena L (2001) Transgenic citrus plants expressing the Citrus tristeza virus p23 protein exhibit viral-like symptoms. Mol Plant Pathol 2:27–36CrossRef

13.

Gibbs MJ, Armstrong JS, Gibbs AJ (2000) Sister-scanning: a Monte Carlo procedure for assessing signals in recombinant sequences. Bioinformatics 16:573–582CrossRefPubMed

14.

Gillings M, Broadbent P, Indsto J, Lee RF (1993) Characterisation of isolates and strains of Citrus tristeza clostero virus using restriction analysis of the coat protein gene amplified by the polymerase chain reaction. J Virol Methods 44:305–317CrossRefPubMed

15.

Guindon S, Gascuel O (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52:696–704CrossRefPubMed

16.

Guo HS, Ding SW (2002) A viral protein inhibits the long range signaling activity of the gene silencing signal. EMBO J 21:398–407CrossRefPubMed

17.

Harper SJ, Dawson TE, Mooney PA, Pearson MN (2008) Molecular analysis of the coat protein and minor coat protein genes of New Zealand Citrus tristeza virus isolates that overcome the resistance of Poncirus trifoliata (L.) Raf. Australas Plant Pathol 37:379–386CrossRef

18.

Harper SJ, Dawson TE, Pearson MN (2009) Complete genome sequences of two distinct and diverse Citrus tristeza virus isolates from New Zealand. Arch Virol 154:1505–1510CrossRefPubMed

19.

Hilf ME, Karasev AV, Pappu HR, Gumpf DJ, Niblett CL, Garnsey SM (1995) Characterization of Citrus tristeza virus subgenomic RNAs in infected tissue. Virology 208:576–582CrossRefPubMed

20.

Huson DH, Bryant D (2006) Application of phylogenetic networks in evolutionary studies. Mol Biol Evol 23:254–267CrossRefPubMed

21.

Karasev AV, Boyko VP, Gowda S, Nikolaeva OV, Hilf ME, Koonin EV, Niblett CL, Cline K, Gumpf DJ, Lee RF, Garnsey SM, Lewandowski DJ, Dawson WO (1995) Complete sequence of Citrus tristeza virus RNA genome. Virology 208:511–520CrossRefPubMed

22.

Kasschau KD, Carrington JC (2001) Long-distance movement and replication maintenance functions correlate with silencing suppression activity of potyviral HC-Pro. Virology 285:71–81CrossRefPubMed

23.

Kosakovsky Pond SL, Frost SDW (2005) Not so different after all: a comparison of methods for detecting amino acid sites under selection. Mol Biol Evol 22:1208–1222CrossRefPubMed

24.

Kosakovsky Pond SL, Frost SDW (2005) Datamonkey: rapid detection of selective pressure on individual sites of codon alignments. Bioinformatics 21:2531–2533CrossRef

25.

Kosakovsky Pond SL, Frost SDW (2005) A genetic algorithm approach to detecting lineage-specific variation in selection pressure. Mol Biol Evol 22:478–485CrossRef

26.

Lu R, Folimonov A, Shintaku M, Li WX, Falk BW, Dawson WO, Ding SW (2004) Three distinct suppressors of RNA silencing encoded by a 20-kb viral RNA genome. Proc Natl Acad Sci USA 101:15742–15747CrossRefPubMed

27.

Martin D, Rybicki E (2000) RDP: detection of recombination amongst aligned sequences. Bioinformatics 16:562–563CrossRefPubMed

28.

Martin DP, Posada D, Crandall KA, Williamson C (2005) A modified bootscan algorithm for automated identification of recombinant sequences and recombination breakpoints. AIDS Res Hum Retroviruses 21:98–102CrossRefPubMed

29.

Martin DP, Williamson C, Posada D (2005) RDP2: recombination detection and analysis from sequence alignments. Bioinformatics 21:260–262CrossRefPubMed

30.

Martin S, Sambade A, Rubio L, Vives MC, Moya P, Guerri J, Elena SF, Moreno P (2009) Contribution of recombination and selection to molecular evolution of Citrus tristeza virus. J Gen Virol 90:1527–1538CrossRefPubMed

31.

Mawassi M, Mietkiewska E, Gofman R, Yang G, Bar-Joseph M (1996) Unusual sequence relationships between two isolates of Citrus tristeza virus. J Gen Virol 77:2359–2364CrossRefPubMed

32.

Mestre PF, Asins MJ, Carbonell EA, Navarro L (1997) New gene(s) involved in the resistance of Poncirus trifoliata (L.) Raf. to Citrus tristeza virus. Theor Appl Genet 95:691–695CrossRef

33.

Mooney PA, Dawson TE, Niblett CL (2000) Comparison of the coat protein gene sequences of Citrus tristeza virus in New Zealand. In: Yokomi RK, Lee RF, Da Graca JV (eds) Proceedings of the 14th conference of the International Organisation of citrus virologists. IOCV, Riverside, pp 28–33

34.

Moreno P, Ambros S, Albiach-Marti MR, Guerri J, Pena L (2008) Citrus tristeza virus: a pathogen that changed the course of the citrus industry. Mol Plant Pathol 9:251–268CrossRefPubMed

35.

Nagy PD, Simon AE (1997) New insights into the mechanisms of RNA recombination. Virology 135:1–9CrossRef

36.

Navas-Castillo J, Albiach-Marti MR, Gowda S, Hilf ME, Garnsey SM, Dawson WO (1997) Kinetics of accumulation of Citrus tristeza virus RNAs. Virology 228:92–97CrossRefPubMed

37.

Peremyslov VV, Hagiwara Y, Dolja VV (1999) HSP70 homolog functions in cell-to-cell movement of a plant virus. Proc Natl Acad Sci USA 96:14771–14776CrossRefPubMed

38.

Posada D, Crandall KA (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 14:817–818CrossRefPubMed

39.

Posada D, Crandall KA (2001) Evaluation of methods for detecting recombination from DNA sequences: computer simulations. Proc Natl Acad Sci USA 98:13757–13762CrossRefPubMed

40.

Powell CA, Lin Y (2005) Separation of Citrus tristeza virus isolates in mixed infections through transfer by single brown citrus aphids. Hortscience 40:693–696

41.

Rao ALN, Hall TC (1993) Recombination and polymerase error facilitate restoration of infectivity in Brome mosaic virus. J Virol 67:969–979PubMed

42.

Reed JC, Kassckau KD, Prokhnevsky AI, Gopinath K, Pogue GP, Carrington JC, Dolja VV (2003) Suppressor of RNA silencing encoded by Beet yellows virus. Virology 206:203–209CrossRef

43.

Roy A, Brlansky RH (2005) Genomic sequence of Citrus tristeza virus from India represents a new genotype. Phytopathology 96:S101

44.

Rubio L, Ayllon MA, Guerri J, Pappu HR, Niblett CL, Moreno P (1996) Differentiation of Citrus tristeza closterovirus (CTV) isolates by single-strand conformation polymorphism analysis of the coat protein gene. Ann Appl Biol 129:479–489CrossRef

45.

Rubio L, Ayllon MA, Kong P, Fernandez A, Polek M, Guerri J, Moreno P, Falk BW (2001) Genetic variation of Citrus tristeza virus isolates from California and Spain: evidence for mixed infections and recombination. J Virol 75:8054–8062CrossRefPubMed

46.

Ruiz-Ruiz S, Moreno P, Guerri J, Ambros S (2006) The complete nucleotide sequence of a severe stem pitting isolate of Citrus tristeza virus from Spain: comparison with isolates from different origins. Arch Virol 151:387–398CrossRefPubMed

47.

Satyanarayana T, Gowda S, Boyko VP, Albiach-Marti MR, Mawassi M, Navas-Castillo J, Karasev AV, Dolja VV, Hilf ME, Lewandowski DJ, Moreno P, Bar-Joseph M, Garnsey SM, Dawson WO (1999) An engineered closterovirus RNA replicon and analysis of heterologous terminal sequences for replication. Proc Natl Acad Sci USA 96:7433–7448CrossRefPubMed

48.

Satyanarayana T, Gowda S, Mawassi M, Albiach-Marti MR, Ayllon MA, Robertson C, Garnsey SM, Dawson WO (2000) Closterovirus encoded HSP70 homolog and p61 in addition to both coat proteins function in efficient virion assembly. Virology 278:253–265CrossRefPubMed

49.

Satyanarayana T, Gowda S, Ayllon MA, Albiach-Marti MR, Rabindran S, Dawson WO (2002) The p23 protein of Citrus tristeza virus controls asymmetrical RNA accumulation. J Virol 76:473–483CrossRefPubMed

50.

Satyanarayana T, Gowda S, Ayllon MA, Dawson WO (2004) Closterovirus bipolar virion: evidence for initiation of assembly by minor coat protein and its restriction to the genomic RNA 5′ region. Proc Natl Acad Sci USA 101:799–804CrossRefPubMed

51.

Swofford DL (2003) PAUP*: phylogenetic analysis using parsimony (* and other methods), version 4.0b 10. Sinauer Associates, Sunderland

52.

Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 10:1093

53.

Tatineni S, Robertson CJ, Garnsey SM, Bar-Joseph M, Gowda S, Dawson WO (2008) Three genes of Citrus tristeza virus are dispensable for infection and movement throughout some varieties of citrus trees. Virology 376:297–307CrossRefPubMed

54.

Vives MC, Rubio L, Lopez C, Navas-Castillo J, Albiach-Marti MR, Dawson WO, Guerri J, Flores R, Moreno P (1999) The complete genome sequence of the major component of a mild Citrus tristeza virus isolate. J Gen Virol 80:811–816PubMed

55.

Vives MC, Rubio L, Sambade A, Mirkov TE, Moreno P, Guerri J (2005) Evidence of multiple recombination events between two RNA sequence variants within a Citrus tristeza virus isolate. Virology 331:232–237CrossRefPubMed

56.

Yoshida T (1985) Inheritance of susceptibility to Citrus tristeza virus in trifoliate orange (Poncirus trifoliata Raf.) (In Japanese). Bull Fruit Tree Res Stn B 12:17–25 (abstract)

57.

Zuker M (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31:3406–3415CrossRefPubMed

Titel: Isolates of Citrus tristeza virus that overcome Poncirus trifoliata resistance comprise a novel strain
verfasst von: S. J. Harper
T. E. Dawson
M. N. Pearson
Publikationsdatum: 01.04.2010
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 4/2010
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-010-0604-5

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