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Archives of Virology

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01.02.2012 | Brief Report

Differentiation of infectious bursal disease virus (IBDV) genome segment B of very virulent and classical lineage by RT-PCR amplification and restriction enzyme analysis

verfasst von: M. R. Islam, S. Rahman, M. Noor, E. H. Chowdhury, H. Müller

Erschienen in: Archives of Virology | Ausgabe 2/2012

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Abstract

Molecular characterization of IBDV usually relies on the analysis of segment A of the bi-segmented, double-stranded RNA genome. Although segments B of classical and very virulent IBDVs differ significantly, re-assortment of genome segments does occur, and molecular epidemiological studies demand the analysis of both segments. An RT-PCR and restriction enzyme analysis for molecular discrimination between genome segment B of classical and very virulent IBDVs is described. Tested on eight IBDV strains/isolates, the protocol successfully identified very virulent and classical IBDVs as well as a segment reassortant. This approach is a valuable tool for molecular epidemiological studies on IBDV.

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Titel: Differentiation of infectious bursal disease virus (IBDV) genome segment B of very virulent and classical lineage by RT-PCR amplification and restriction enzyme analysis
verfasst von: M. R. Islam
S. Rahman
M. Noor
E. H. Chowdhury
H. Müller
Publikationsdatum: 01.02.2012
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 2/2012
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-011-1159-9

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