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Archives of Virology

Erschienen in:

01.05.2014 | Original Article

Production and immunogenicity of VP2 protein of porcine parvovirus expressed in Pichia pastoris

verfasst von: Chunhe Guo, Zemin Zhong, Yumao Huang

Erschienen in: Archives of Virology | Ausgabe 5/2014

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Abstract

Viral protein 2 (VP2) of porcine parvovirus (PPV) is the major viral structural protein and is responsible for eliciting neutralizing antibodies in immunized animals. In this study, we constructed and characterized a recombinant yeast vector encoding the VP2 protein, designated as pGAPZαA-VP2. The construct was confirmed by restriction enzyme digestion, PCR, and sequencing and then introduced into P. pastoris strain SMD1168 by electroporation. The expressed VP2 protein was analyzed by SDS-PAGE and western blot. Immunization of mice with the VP2 protein elicited a PPV-specific humoral immune response. Notably, a preparation of VP2 protein containing adjuvant induced a much better antibody response than VP2 alone. Clearly, the adjuvant strongly enhanced the immunogenicity of VP2. This study provides a foundation for the application of the VP2 protein in the clinical diagnosis of PPV and in vaccination against PPV in the future.

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Titel: Production and immunogenicity of VP2 protein of porcine parvovirus expressed in Pichia pastoris
verfasst von: Chunhe Guo
Zemin Zhong
Yumao Huang
Publikationsdatum: 01.05.2014
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 5/2014
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-013-1907-0

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