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01.07.2014 | Original Article

Generation and characterization of potential dengue vaccine candidates based on domain III of the envelope protein and the capsid protein of the four serotypes of dengue virus

verfasst von: Edith Suzarte, Ernesto Marcos, Lázaro Gil, Iris Valdés, Laura Lazo, Yassel Ramos, Yusleidi Pérez, Viviana Falcón, Yaremis Romero, María G. Guzmán, Sirenia González, Juan Kourí, Gerardo Guillén, Lisset Hermida

Erschienen in: Archives of Virology | Ausgabe 7/2014

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Abstract

Dengue is currently one of the most important arthropod-borne diseases, causing up to 25,000 deaths annually. There is currently no vaccine to prevent dengue virus infection, which needs a tetravalent vaccine approach. In this work, we describe the cloning and expression in Escherichia coli of envelope domain III-capsid chimeric proteins (DIIIC) of the four dengue serotypes as a tetravalent dengue vaccine candidate that is potentially able to generate humoral and cellular immunity. The recombinant proteins were purified to more than 85 % purity and were recognized by anti-dengue mouse and human sera. Mass spectrometry analysis verified the identity of the proteins and the correct formation of the intracatenary disulfide bond in the domain III region. The chimeric DIIIC proteins were also serotype-specific, and in the presence of oligonucleotides, they formed aggregates that were visible by electron microscopy. These results support the future use of DIIIC recombinant chimeric proteins in preclinical studies in mice for assessing their immunogenicity and efficacy.

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Titel: Generation and characterization of potential dengue vaccine candidates based on domain III of the envelope protein and the capsid protein of the four serotypes of dengue virus
verfasst von: Edith Suzarte
Ernesto Marcos
Lázaro Gil
Iris Valdés
Laura Lazo
Yassel Ramos
Yusleidi Pérez
Viviana Falcón
Yaremis Romero
María G. Guzmán
Sirenia González
Juan Kourí
Gerardo Guillén
Lisset Hermida
Publikationsdatum: 01.07.2014
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 7/2014
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-013-1956-4

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