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25.11.2017 | Original Article

Porcine teschovirus 2 induces an incomplete autophagic response in PK-15 cells

verfasst von: Yuanxing Gu, Yingshan Zhou, Xinfeng Shi, Yongping Xin, Ying Shan, Cong Chen, Tong Cao, Weihuan Fang, Xiaoliang Li

Erschienen in: Archives of Virology | Ausgabe 3/2018

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Abstract

Autophagy is a homeostatic process that has been shown to be vital in the innate immune defense against pathogens. However, little is known about the regulatory role of autophagy in porcine teschovirus 2 (PTV-2) replication. In this study, we found that PTV-2 infection induces a strong increase in GFP-LC3 punctae and endogenous LC3 lipidation. However, PTV-2 infection did not enhance autophagic protein degradation. When cellular autophagy was pharmacologically inhibited by wortmannin or 3-methyladenine, PTV-2 replication increased. The increase in virus yield via autophagy inhibition was further confirmed by silencing atg5, which is required for autophagy. Furthermore, PTV-2 replication was suppressed when autophagy was activated by rapamycin. Together, the results suggest that PTV-2 infection activates incomplete autophagy and that autophagy then inhibits further PTV-2 replication.

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Titel: Porcine teschovirus 2 induces an incomplete autophagic response in PK-15 cells
verfasst von: Yuanxing Gu
Yingshan Zhou
Xinfeng Shi
Yongping Xin
Ying Shan
Cong Chen
Tong Cao
Weihuan Fang
Xiaoliang Li
Publikationsdatum: 25.11.2017
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 3/2018
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-017-3652-2

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