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Documenta Ophthalmologica

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01.06.2014 | Original Research Article

Contribution of retinal ganglion cells to the mouse electroretinogram

verfasst von: Benjamin J. Smith, Xu Wang, Balwantray C. Chauhan, Patrice D. Côté, François Tremblay

Erschienen in: Documenta Ophthalmologica | Ausgabe 3/2014

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Abstract

Purpose

To quantify the direct contribution of retinal ganglion cells (RGCs) on individual components of the mouse electroretinogram (ERG).

Methods

Dark- and light-adapted ERGs from mice 8 to 12 weeks after optic nerve transection (ONTx, n = 14) were analyzed through stimulus response curves for a- and b-waves, oscillatory potentials (OPs), positive and negative scotopic threshold response (p/n STR), and the photopic negative response (PhNR) and compared with unoperated and sham-operated controls, as well as to eyes treated with 6-cyano-7-nitroquinoxaline-2,3-dion (CNQX).

Results

We confirmed in mice that CNQX intravitreal injection reduced the scotopic a-wave amplitude at high flash strength, confirming a post-receptoral contribution to the a-wave. We found that ONTx, which is more specific to RGCs, did not affect the a-wave amplitude and implicit time in either photopic or scotopic conditions while the b-wave was reduced. Both the pSTR and nSTR components were reduced in amplitude, with the balance between the two components resulting in a shortening of the nSTR peak implicit time. On the other hand, amplitude of the PhNR was increased while the OPs were minimally affected.

Conclusion

With an intact a-wave demonstrated following ONTx, we find that the most robust indicators of RGC function in the mouse full-field ERG were the STR components.

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Titel: Contribution of retinal ganglion cells to the mouse electroretinogram
verfasst von: Benjamin J. Smith
Xu Wang
Balwantray C. Chauhan
Patrice D. Côté
François Tremblay
Publikationsdatum: 01.06.2014
Verlag: Springer Berlin Heidelberg
Erschienen in: Documenta Ophthalmologica / Ausgabe 3/2014
Print ISSN: 0012-4486
Elektronische ISSN: 1573-2622
DOI: https://doi.org/10.1007/s10633-014-9433-2

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Contribution of retinal ganglion cells to the mouse electroretinogram

Abstract

Purpose

Methods

Results

Conclusion

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