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Journal of Assisted Reproduction and Genetics

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01.04.2015 | Gamete Biology

Effect of L-carnitine supplementation on maturation and early embryo development of immature mouse oocytes selected by brilliant cresyle blue staining

verfasst von: Zohreh Zare, Reza Masteri Farahani, Mohammad Salehi, Abbas Piryaei, Marefat Ghaffari Novin, Fatemeh Fadaei Fathabadi, Moslem Mohammadi, Maryam Dehghani-Mohammadabadi

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 4/2015

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Abstract

Purpose

The present study was designed to investigate the effect of L-carnitine treatment during IVM on nuclear and cytoplasmic maturation of immature oocytes selected by Brilliant Cresyle Blue (BCB) staining, and their subsequent developmental competence.

Materials & methods

Compact cumulus-oocyte complexes (COCs) were collected from NMRI mice ovaries and stained with BCB staining. BCB+ (colored cytoplasm) oocytes were then cultured in tissue culture medium (TCM) 199 with 0.0, 0.3 and 0.6 mg/ml L-carnitine.

Results

The both L-carnitine concentrations significantly increased the intracellular glutathione (P < 0.001), nuclear maturation (P < 0.01) and expression levels of cyclin-dependent kinase1 (CDK1) (P < 0.05). Moreover, treated oocytes with 0.6 mg/ml L-carnitine showed increased (P < 0.05) expression of mitogen-activated protein kinase1 (MAPK1) mRNA. Also, adding L-carnitine (0.6 mg/ml) to IVM medium significantly increased the cleavage rate (P < 0.05). The blastocyst development rate (BDR) in the both L-carnitine treated groups was significantly higher (P < 0.001) than the control group. L-carnitine had no significant effect on total blastocyst cell numbers.

Conclusions

These data indicated that L-carnitine supplementation during IVM of immature BCB+ oocytes improved preimplantation developmental competence of oocytes after IVF, probably by accelerating cytoplasmic and nuclear maturation of oocytes. It may provide a novel approach to improving ART outcomes in infertile couples.

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Titel: Effect of L-carnitine supplementation on maturation and early embryo development of immature mouse oocytes selected by brilliant cresyle blue staining
verfasst von: Zohreh Zare
Reza Masteri Farahani
Mohammad Salehi
Abbas Piryaei
Marefat Ghaffari Novin
Fatemeh Fadaei Fathabadi
Moslem Mohammadi
Maryam Dehghani-Mohammadabadi
Publikationsdatum: 01.04.2015
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 4/2015
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-015-0430-5

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Effect of L-carnitine supplementation on maturation and early embryo development of immature mouse oocytes selected by brilliant cresyle blue staining

Abstract

Purpose

Materials & methods

Results

Conclusions

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Abstract

Purpose

Materials & methods

Results

Conclusions

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