Erschienen in:
01.05.2007 | Original Paper
CD4
+CD25
+ Regulatory T Cells Decreased the Antitumor Activity of Cytokine-Induced Killer (CIK) Cells of Lung Cancer Patients
verfasst von:
Hui Li, Jin-Pu Yu, Shui Cao, Feng Wei, Peng Zhang, Xiu-Mei An, Zong-Tang Huang, Xiu-Bao Ren
Erschienen in:
Journal of Clinical Immunology
|
Ausgabe 3/2007
Einloggen, um Zugang zu erhalten
CD4
+CD25
+ regulatory T cells (Tregs) have been shown to inhibit cytotoxic lymphocytes-mediated immune responses. Cytokine-induced killer (CIK) cells exert high impact on adoptive immunotherapeutic approaches. Therefore, the purpose of this report was to determine the effect of Tregs on CIK cell growth and CIK-induced cytotoxicity for inhibition of tumor growth in vivo as well as in vitro. After depletion of CD4
+CD25
+ cells before culture, the proliferation and cytotoxicity of CIK cells, which indicated in bromodeoxyuridine (BrdU) and lactic dehydrogenase (LDH) assays, were significantly increased. Depletion of CD4
+CD25
+ cells preculture also enhanced the suppression effect on the lung cancer cells inoculated in experimental animals. Blockage of glucocorticoid-induced tumor necrosis factor receptor (GITR) and transforming growth factor β1 (TGF-β1) by antibodies partially abrogated the suppressive effect of CD4
+CD25
+ cells on CIK. These results indicated that Tregs could inhibit the antitumor activity of CIK cells. The molecules TGF-β and GITR may contribute to the suppressive function of CD4
+CD25
+ cells.