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Journal of Natural Medicines

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01.01.2014 | Original Paper

Berberine inhibits the growth of human colorectal adenocarcinoma in vitro and in vivo

verfasst von: Yuchen Cai, Qing Xia, Rongzhen Luo, Peiyu Huang, Yueli Sun, Yanxia Shi, Wenqi Jiang

Erschienen in: Journal of Natural Medicines | Ausgabe 1/2014

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Abstract

Berberine is an alkaloid isolated from the Chinese herbal medicine Huanglian, and has long been used as an antibiotic. Its antineoplastic properties were subsequently discovered in vitro. The purpose of this study was to investigate the effects of berberine on the growth of human colorectal carcinoma cells in vitro and in vivo. The results showed that berberine inhibited human colorectal adenocarcinoma (LoVo) cell growth in a time- and dose-dependent manner. A WST-1 assay showed that the IC₅₀ value after 72 h was 40.79 ± 4.11 μM. Cell cycle analysis of 40 μM berberine-treated LoVo cells by flow cytometry showed accumulation of cells in the G₂/M phase. The inhibition of LoVo cell growth by berberine was associated with the suppression of cyclin B1, cdc2, and cdc25c proteins. Berberine at a dose of 50 mg kg⁻¹ day⁻¹ showed inhibitory rates of 45.3 % in a human colorectal adenocarcinoma xenograft in nude mice. The combination of berberine and 5-fluorouracil (5-FU) had a higher inhibitory rate (59.8 %) than the berberine group (36.4 %, P = 0.01), but no significant difference was observed between the 5-FU group (43.0 %, P = 0.06) and the combination group. These results support the possibility that berberine may be useful as an alternative therapy for colorectal carcinoma.

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Titel: Berberine inhibits the growth of human colorectal adenocarcinoma in vitro and in vivo
verfasst von: Yuchen Cai
Qing Xia
Rongzhen Luo
Peiyu Huang
Yueli Sun
Yanxia Shi
Wenqi Jiang
Publikationsdatum: 01.01.2014
Verlag: Springer Japan
Erschienen in: Journal of Natural Medicines / Ausgabe 1/2014
Print ISSN: 1340-3443
Elektronische ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-013-0766-z

Springer Medizin

Abstract

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