Alcohol and HIV Disease Progression: Weighing the Evidence

Chronic alcohol consumption has been shown to be associated with increased susceptibility to infectious diseases (eg, tuberculosis and bacterial pneumonia), increased severity of diseases (eg, viral hepatitis), and increased risk of cancers (eg, hepatocellular carcinoma) [7]. These illnesses may also be accelerated by vitamin deficiencies, malnutrition, or other substance use. Chronic alcohol consumption can lead to liver disease and cirrhosis, which can impact immunocompetence. Recent animal and human studies have shown that alcohol consumption has deleterious effects on both the innate and the acquired immune responses. Impaired innate immune responses may cause susceptibility to infection, while impaired acquired immune responses such as impaired B lymphocyte function, altered cytokine balance, and chronic T-cell activation may accelerate disease progression, including that caused by HIV. In addition, alcohol may play a role in translocation of bacteria and bacterial products from the gut to cause HIV immune activation, resulting in increased HIV disease progression [8].

Micro-nutrient (ie, vitamin and mineral) deficiencies, including selenium and vitamins D, A, B-12, and E, zinc, and iron, have been associated with more rapid HIV disease progression [9‐11]. At the same time, antiretroviral therapy (ART) is thought to decrease some and restore other micronutrient levels [12, 13]. Overall nutritional status has been associated with HIV progression in several prospective studies [14‐16], while there is also an effect of HIV infection on nutritional status [17]. Alcohol consumption is associated with nutritional deficiencies, due to a high percentage of caloric intake from alcohol, decreased absorption of nutrients, and interference with the metabolism of nutrients [18]. Therefore, it is plausible that nutritional deficiency is a mechanism by which alcohol might result in more rapid HIV disease progression. The link between alcohol use, decreased nutrition, and immune markers has been demonstrated experimentally in the SIV model [19]; however, it is not clear for HIV infection in humans.

Some ART, including non-nucleoside reverse transcriptase inhibitors and protease inhibitors, is metabolized by the human cytochrome P450 system. There is evidence that alcohol may impact the metabolism of these medications by two different mechanisms, enzymatic induction [20], associated with chronic alcohol use, and enzymatic inhibition due to competition of ethanol for various cytochrome P450 isozymes, associated with acute ethanol use [21]. Because numerous drugs are metabolized by the P450 pathway, chronic alcohol users may be at higher risk for drug toxicities and ineffective therapy due to inadequate plasma drug concentration. Chronic alcohol consumption may also alter drug protein binding. We examine the evidence as to whether heavy alcohol consumption reduces the effectiveness of ART as measured by failure to achieve viral suppression, even with good adherence.

Researchers in the United States and internationally have found that heavy drinkers are less likely than others to be receiving ART [22•, 23, 24••]. This may be a consequence of barriers to consistent medical treatment or due to physicians’ impressions that heavy drinkers are unable to competently use ART. Indeed, heavy alcohol use was independently associated with lower retention in care among indigent patients [25]. Because early and consistent HIV care and receipt of opportunistic infection (OI) prophylaxis and ART are key factors in slowing the progression of HIV [26], these findings suggest one mechanism by which heavy alcohol consumption may accelerate HIV disease progression.

Alcohol consumption has been consistently associated with poorer ART adherence. A recent meta-analysis found that those who used alcohol or drank relatively more were 50%–60% as likely to be classified as adherent compared with those who abstained or drank relatively less [27•]. Some studies have found a dose-response relationship between alcohol consumption and ART adherence [24••, 28], in addition to a temporal association between drinking episodes and missed doses [28]. As ART adherence is a known predictor of HIV outcome, this strongly suggests that decreased adherence is one mechanism by which alcohol may increase HIV disease progression. These findings elevate the importance of attempting to control for ART adherence in examining the biological impact of alcohol on HIV disease progression apart from its impact on adherence. However, adherence is challenging to accurately measure and therefore may pose problems even if included in statistical models. Alternatively and likely more effectively, studies of persons infected with HIV who are not yet on ART may reveal key insights about this important issue.

Comorbidities may complicate the issue of alcohol’s impact on HIV disease progression and make it difficult to determine the association. For example, depression, which can be exacerbated by the effects of alcohol [29], has been shown to reduce adherence to ART [30], and depression, stress, and trauma may have worsening effects on HIV progression [30, 31].

Illicit substance use is strongly associated with heavy alcohol consumption, especially in minority and inner-city populations. Several studies have shown associations between illicit substance use, especially crack cocaine, and HIV progression [32•, 33]. Substance use other than alcohol has also been associated with lower rates of receipt of ART [24••]. Hence, controlling for other non-alcohol substance use is important in seeking an understanding of the impact of alcohol consumption on HIV disease progression.

The direct impact of alcohol consumption on HIV disease progression has been studied using animal models. The strengths of these studies include the absence of ART adherence as a possible mediator of disease progression, and the capacity to control the quantity of alcohol consumed as well as behavioral factors (eg, nutrition), which are not easily accounted for in human studies. Evidence from studies in macaques suggests that heavy alcohol consumption has consequences for increased SIV progression. Experimental administration of the equivalent of heavy doses of alcohol as compared to sucrose to macaques infected with SIV was associated with early plasma CD4 cell loss in some studies [34, 35] but not others [36, 37], while several studies found greater SIV viral load at various times post-infection [19, 34, 36, 37]. Bagby et al. [36] found a significantly more rapid onset of end-stage disease in eight alcohol-administered macaques compared with eight controls. Higher viral load in alcohol-exposed macaques was associated with a higher percentage of SIV target cells (CD4) in the gut coupled with lower percentages of CD8 cells, creating a blunted mucosal immune response in early infection in one study [37]. The alcohol-exposed group consumed significantly fewer calories than the controls in another study [19]. Taken together, these findings suggest a biologically deleterious effect of heavy alcohol administration on disease progression in SIV-infected primates.

A number of clinical studies assessed alcohol use in cross-sectional and prospective analyses. Associations between HIV outcomes such as CD4 cell counts and HIV viral load in cross-sectional studies may reflect differences in the time of study entry by alcohol consumption category, therefore we will focus in this review on prospective studies. Several prospective studies were published using data collected in the pre-HAART era, as described below.

An analysis conducted in the Multicenter AIDS Cohort Study (MACS) included 1706 HIV-infected men, and examined alcohol consumption by average number of drinks per day, ranging from zero to greater than two, the latter meeting the threshold for “heavy” drinking in men. There was no association between drinks per day both at enrollment and prospectively and the development of AIDS [38]. A later analysis of the MACS determined that decreasing alcohol consumption (ie, having a significantly negative slope in the average number of drinks per week in the prior 6 months) was associated with developing AIDS-related conditions, suggesting a decrease in alcohol consumption as HIV progressed [39]. Studies conducted in men in the Netherlands [40] and Norway [41] found no association between the number of drinks per day in the prior 6 months or daily drinking, respectively, with the development of AIDS. A study of vitamin deficiencies (n = 312) reported that in a multivariate model that included age, HIV-related symptoms, baseline CD4 cell count, and several vitamin concentrations, frequent alcohol consumption (>2 times/week) at study baseline was associated with increased time to CD4 cell count declines to 200 cells/mm³ and time to AIDS [42]. A study of 403 persons seroconverting during the Tricontinental Seroconverter Study found that any alcohol use during the first three quarters of the follow-up period, limited to avoid the feedback loop between symptoms and alcohol consumption, was not associated with time to AIDS or death [43]. The selection of “any alcohol use” as a main independent variable, as in the latter study, is a coarse measure to assess alcohol’s impact on HIV disease progression. All of the preceding studies were conducted among men who had sex with men.

Two early studies were conducted among injecting drug users. One study among 496 HIV-infected methadone maintenance patients found no association between daily alcohol consumption in the prior month and time to AIDS or death in a time-dependent multivariate model that included age, sex, CD4 cell count, zidovudine use, having two or more symptoms, and crack cocaine use, while crack cocaine use was independently associated with progression to AIDS [44]. A study conducted among 188 injection drug users found that very heavy alcohol consumption (>21 drinks per week) at baseline was associated with increased %CD8 cells 2–5 years after seroconversion; no impact on CD4 cell count or %CD4 was found [45].

The only study of the issue from a developing country was a cohort study of 105 HIV clinic patients who were not on ART, conducted in Zimbabwe. This study showed no association between any alcohol consumption at baseline and successive CD4 cell count and HIV viral loads over a period of 6 months; however, follow-up was quite limited and changes in these outcomes were not examined [46].

In summary, in the pre-HAART era, no association of alcohol use with more rapid HIV disease progression was identified; however, some studies’ measurement of alcohol consumption was limited in detail or only measured at baseline, and the studies largely examined men.

Studies conducted after the introduction of HAART differed from the earlier studies in that most used CD4 cell count and/or HIV viral load as the study outcomes, an understandable strategy given the reduction in OI and death in the HAART era. Notably, these studies either stratified by or controlled for ART use, and the measurement of alcohol use was more detailed than in the previous studies.

Chander et al. [24••] reported observations among 1711 persons enrolled in an urban HIV clinic cohort from 1998–2003. They found that heavy alcohol use in the prior 6 months alone and combined with injection drug use was associated with decreased viral load suppression after adjusting for age, race, nadir CD4 cell count, and years on ART. Controlling for self-reported adherence in these analyses attenuated the effect of heavy drinking, providing evidence for the causal chain between alcohol and HIV outcomes via ART adherence. Because the attenuation of the effect from a 24% to a 14% reduction in odds of viral suppression was accompanied by somewhat wider confidence intervals and due to the imprecise nature of ART adherence measurement, these data are inconclusive as to whether there is an effect of alcohol on viral suppression beyond that attributed to poorer adherence.

An analysis of participants in two cohorts of a total of 595 HIV-infected persons with a history of alcohol problems examined CD4 cell counts and HIV viral loads at 6-month intervals for up to 7 years [5••]. Upon regression analysis, among subjects not on ART, heavy alcohol consumption was associated with a lower CD4 cell count, on average a difference of 49 cells/mm³. There was no association between heavy alcohol consumption and CD4 cell count among those on ART, in analyses that adjusted for baseline CD4, adherence to ART, homelessness, depressive symptoms, and several other variables. Heavy alcohol use was not associated with HIV viral load in those on ART and those not on ART. All analyses among those on ART adjusted for 3-day self-reported adherence, suggesting that there is no detectable alcohol effect beyond the effect on adherence among those on ART. However, the CD4 cell count difference suggests that there might be an effect of heavy alcohol consumption on HIV progression among those not on ART.

A recent publication by Baum et al. [47••] examined the association between alcohol consumption and HIV outcomes in a cohort of active alcohol or illicit drug users. In this study, frequent alcohol consumption (defined as ≥ 2 drinks/day on average) compared to moderate alcohol use and abstention was associated with a decline of CD4 cell count to less than 200 cells/mm³, among those who had a baseline CD4 cell count of greater than 200 cells/mm³. The model in this study controlled for baseline CD4 cell count, HIV viral load, ART status, years since tested HIV positive, age, and gender. A similar model that examined the same factors but was restricted to those not on ART showed a stronger association. The effect size was also larger when the predictor variable was the combination of frequent alcohol use and crack cocaine use; however, the independent effects of alcohol and crack cocaine use were not shown. In addition, frequent alcohol consumption was associated with increased HIV viral load in a multivariate model controlling for the same variables as above except viral load. However, when stratifying by ART the association was significant only among those on ART, and the authors suggested that the association was mediated by adherence. These analyses are in contrast to a recent analysis of the same cohort, which found that crack cocaine use but not alcohol use, coded only as current yes versus no, was associated with HIV progression [33]. This illustrates the importance of using a more detailed alcohol consumption history to ascertain the relationship of alcohol use and HIV disease progression.

Two studies in women in the post-HAART era failed to find an association between alcohol consumption and HIV outcomes. A recent study of 516 women in the HIV Epidemiologic Research Study (HERS) cohort examined the effects of alcohol consumption (ie, none, moderate, and heavy) on both depressive symptoms and CD4 cell count [48••]. The analysis showed significant associations between both moderate and heavy alcohol consumption and depressive symptoms and between depressive symptoms and CD4 cell count. The direct association between alcohol consumption and CD4 cell count was not statistically significant. The indirect effects of alcohol consumption on CD4 cell count via depression were not reported; therefore, we cannot comment on effect of alcohol on CD4 cell count via the effect on depression. In addition, a large study of 1686 HIV-positive women in the Women’s Interagency HIV Study (WIHS) found that there was no positive association between heavy alcohol consumption and time to newly acquired AIDS-defining illnesses or AIDS-related death, in repeated measures models that adjusted for crack use, ART use and adherence, CD4 cell count at baseline, HIV viral load at baseline, year of HIV diagnosis, and demographic variables [32•]. This study found a strong association of persistent crack use and AIDS-related mortality and both persistent and intermittent crack use and newly acquired AIDS illnesses. This result is consistent with other studies that included crack use in multivariate models [33, 34].

Lastly, a study conducted multivariate modeling of the effect of drink types on HIV viral load suppression, CD4 cell count, and thymus volume in 165 patients after 24 weeks of ART [49••]. In models that controlled for demographics, baseline Centers for Disease Control and Prevention HIV stage, and adherence, heavy alcohol consumption was not associated with the outcomes while consuming predominantly liquor compared to beer or wine was associated with lack of HIV viral suppression, decreased thymus size, and change in CD4 cell count. This study highlights a potential future area of interest, that is, impact of alcohol beverage type.

In summary, we identified six studies in the post-HAART era, and three demonstrated an association between heavy alcohol use and at least one measure of HIV disease progression [5••, 24••, 47••].