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Clinical Orthopaedics and Related Research®

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01.11.2010 | Basic Research

Increased Chondrocyte Death after Steroid and Local Anesthetic Combination

verfasst von: Boglárka Farkas, MD, Krisztián Kvell, MD, PhD, Tamás Czömpöly, MD, PhD, Tamás Illés, MD, PhD, Tamás Bárdos, MD, PhD

Erschienen in: Clinical Orthopaedics and Related Research® | Ausgabe 11/2010

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Abstract

Background

Hyaline articular cartilage has limited repair and regeneration capacity. Intraarticular administration of glucocorticoid and local anesthetic injections play an important role in the therapy of osteoarthritis. Glucocorticoids and anesthetics reportedly enhance apoptosis in chondrocytes, but effects of the combined use of glucocorticoids and local anesthetics are unknown.

Questions/purposes

We asked whether glucocorticoid and local anesthetic agents combined had any synergistic effects on chondrocyte apoptosis.

Methods

Cell viability and apoptosis/necrosis assessment of human articular chondrocytes were performed in vitro (chondrocyte cell cultures) and ex vivo (osteochondral specimens) using flow cytometry and TUNEL analysis, respectively.

Results

Glucocorticoids and local anesthetics induce apoptosis in chondrocytes at various rates. When used in combination, the percentage of dead chondrocytes was increased in in vitro chondrocyte cell cultures and osteochondral ex vivo specimens.

Conclusions

We observed a time-dependent decrease in chondrocyte viability after concurrent steroid and local anesthetic exposure.

Clinical Relevance

The combination of glucocorticoids and local anesthetics has an adverse effect on articular chondrocytes, and it raises a question regarding whether concomitant administration should be used in treating osteoarthritis.

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Titel: Increased Chondrocyte Death after Steroid and Local Anesthetic Combination
verfasst von: Boglárka Farkas, MD
Krisztián Kvell, MD, PhD
Tamás Czömpöly, MD, PhD
Tamás Illés, MD, PhD
Tamás Bárdos, MD, PhD
Publikationsdatum: 01.11.2010
Verlag: Springer-Verlag
Erschienen in: Clinical Orthopaedics and Related Research® / Ausgabe 11/2010
Print ISSN: 0009-921X
Elektronische ISSN: 1528-1132
DOI: https://doi.org/10.1007/s11999-010-1443-0

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Increased Chondrocyte Death after Steroid and Local Anesthetic Combination

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