Introduction
Methods
Experimental Design
Plasma Collection
Killing and Tissue and Urine Sample Collection
Plasma Lipid Profiles
RNA Isolation and Quantitative RT-PCR
Analysis of 8-Isoprostane and 8-Hydroxy-2-Deoxyguanosine
Histological Analysis
Results
Filtered air | Diesel exhaust | |
---|---|---|
Total triglyceride (mg/dl)
| ||
8 Weeks | 76.7 ± 42.7
n = 36 | 65.6 ± 25.2
n = 18 |
12 Weeks | 68.5 ± 35.8
n = 26 | 65 ± 24.8
n = 18 |
16 Weeks | 333.4 ± 233.6
n = 18 | 168.4 ± 124.1
n = 15 |
Total cholesterol (mg/dl)
| ||
8 Weeks | 253.9 ± 46.7
n = 35 | 259.5 ± 54.6
n = 18 |
12 Weeks | 322.9 ± 93.5
n = 29 | 330.5 ± 134.1
n = 23 |
16 Weeks | 259.4 ± 131.2
n = 27 | 367.6 ± 116.3
n = 16 |
Gene/tissue | Filtered air | Diesel exhaust |
---|---|---|
GCLc
| ||
Lung (n = 9) | 1.26 ± 0.37 | 0.55 ± 0.05 |
Liver (n = 9) | 1.15 ± 0.25 | 1.04 ± 0.36 |
Aorta (n = 6) | 0.71 ± 0.13 | 0.66 ± 0.4 |
GCLm
| ||
Lung (n = 10) | 1.11 ± 0.22 | 0.78 ± 0.09 |
Liver (n = 10) | 1.08 ± 0.21 | 1.16 ± 0.28 |
Aorta (n = 6) | 0.77 ± 0.11 | 0.76 ± 0.43 |
HMOX1
| ||
Lung (n = 10) | 1.92 ± 0.96 | 0.24 ± 0.03 |
Liver (n = 10) | 1.09 ± 0.20 | 0.94 ± 0.29 |
Aorta (n = 6) | 0.74 ± 0.33 | 0.75 ± 0.46 |
DE | FA | |
---|---|---|
Liver 8-hydroxyguanosine (pg/g) (n = 24/group) | 572.9 | 567.3 |
Urine 8-isoprostane (pg/ml) (n = 24/group) | 127.6 | 284.9 |
No. of mice | Filtered air | Diesel exhaust | ||
---|---|---|---|---|
N
| % |
N
| % | |
Gender
| ||||
Female | 10 | 37 | 8 | 47 |
Male | 17 | 63 | 9 | 53 |
Lesion frequency
| ||||
No. of animals with lesions | 8 | 29 | 10 | 59 |
No. of animals without lesions | 19 | 71 | 7 | 41 |
Average lesion area (µ
2) | 3486.04 ± 2237.7 | 3269.02 ± 1683.4 |
Exposure # | DE litter size | DE females in each litter/males in each litter | FA litter size | FA females in each litter/males in each litter | |
---|---|---|---|---|---|
1 | 5 | 3/2 | 6 | 3/3 | |
1 | 4 | 3/1 | 6 | 0/6 | |
1 | 4 | 3/1 | 7 | 3/4 | |
1 | 6 | 1/5 | 8 | 4/4 | |
1 | 7 | 3/4 | 6 | 3/3 | |
1 | 4 | 4/0 | 8 | 2/6 | |
2 | 4 | 2/2 | 8 | 5/3 | |
2 | 6 | 3/3 | 8 | 3/5 |
First-generation DE total (M and F) (n = 30) | First-generation DE F (n = 15) | First-generation DE M (n = 15) | First-generation FA total (M and F) (n = 41) | First-generation FA F (n = 15) | First-generation FA M (n = 26) |
---|---|---|---|---|---|
9781.9 | 5708.0 | 13,855.8 | 14,311.3 | 19,173.6 | 9449.0 |
Second-generation DE total (M and F) (n = 10) | Second-generation DE F (n = 5) | Second-generation DE M (n = 5) | Second-generation FA total (M and F) (n = 16) | Second-generation FA F (n = 8) | Second-generation FA M (n = 8) |
14,387.8 | 20,067.0 | 8708.7 | 5457.0 | 5188.3 | 5725.6 |