Skip to main content
Hauptrubriken
CME Facharzt-Training Webinare Zeitschriften Bücher e.Medpedia Podcast
Service
Jobbörse Info & Hilfe
Fachgebiete
Anästhesiologie Allgemeinmedizin Arbeitsmedizin Augenheilkunde Chirurgie Dermatologie Gynäkologie und Geburtshilfe HNO Innere Medizin Kardiologie Neurologie Onkologie und Hämatologie Orthopädie und Unfallchirurgie Pädiatrie Pathologie Psychiatrie Radiologie Rechtsmedizin Urologie Zahnmedizin
Themen
Klimawandel und Gesundheit Neues aus dem Markt COVID-19 Praxis und Beruf Seltene Erkrankungen GOÄ & EBM Panorama
Sammlungen
Kasuistiken Algorithmen & Infografiken Blickdiagnosen Arthropedia FlapFinder (für Dermatochirurgen) Videos Kongressberichterstattung Medizin für Apothekerinnen und Apotheker Für Ärztinnen und Ärzte in Weiterbildung Für Medizinstudierende
Erweiterte Suche
Anmelden
nach oben
Endocrine
Erschienen in: Endocrine 1/2014

01.05.2014 | Original Article

Expression of interleukin-15 and inflammatory cytokines in skeletal muscles of STZ-induced diabetic rats: effect of resistance exercise training

verfasst von: M. Molanouri Shamsi, Z. H. Hassan, R. Gharakhanlou, L. S. Quinn, K. Azadmanesh, L. Baghersad, A. Isanejad, M. Mahdavi

Erschienen in: Endocrine | Ausgabe 1/2014

Einloggen, um Zugang zu erhalten

Abstract

Skeletal muscle atrophy is associated with type-1 diabetes. Skeletal muscle is the source of pro- and anti-inflammatory cytokines that can mediate muscle hypertrophy and atrophy, while resistance exercise can modulate both muscle mass and muscle cytokine expression. This study determined the effects of a 5-week resistance exercise training regimen on the expression of muscle cytokines in healthy and streptozotocin-induced diabetic rats, with special emphasis on interleukin-15 (IL-15), a muscle-derived cytokine proposed to be involved in muscle hypertrophy or responses to stress. Induction of diabetes reduced muscle weight in both the fast flexor hallucis longus (FHL) and slow soleus muscles, while resistance training preserved FHL muscle weight in diabetic rats. IL-15 protein content was increased by training in both FHL and soleus muscles, as well as serum, in normal and diabetic rats. With regard to proinflammatory cytokines, muscle IL-6 levels were increased in diabetic rats, while training decreased muscle IL-6 levels in diabetic rats; training had no effect on FHL muscle IL-6 levels in healthy rats. Also, tumor necrosis factor-alpha (TNF-α) and IL-1β levels were increased by diabetes, but not changed by training. In conclusion, we found that in diabetic rats, resistance training increased muscle and serum IL-15 levels, decreased muscle IL-6 levels, and preserved FHL muscle mass.
Literatur
1.
B.C. Frier, E.G. Noble, M. Locke, Diabetes-induced atrophy is associated with a muscle-specific alteration in NF-kappaB activation and expression. Cell Stress Chaperones 13(3), 287–296 (2008)PubMedCentralPubMedCrossRef
2.
H. Andersen, P.C. Gadeberg, B. Brock, J. Jakobsen, Muscular atrophy in diabetic neuropathy: a stereological magnetic resonance imaging study. Diabetologia 40(9), 1062–1069 (1997)PubMedCrossRef
3.
R.A. Frost, C.H. Lang, Regulation of muscle growth by pathogen-associated molecules. J. Anim. Sci. 86(14), 84–93 (2008)CrossRef
4.
G.Q. Chen, C.Y. Mou, Y.Q. Yang, S. Wang, Z.W. Zhao, Exercise training has beneficial anti-atrophy effects by inhibiting oxidative stress-induced MuRF1 upregulation in rats with diabetes. Life Sci. 89(1–2), 44–49 (2011)PubMedCrossRef
5.
R.B. Hunter, E. Stevenson, A. Koncarevic, H. Mitchell-Felton, D.A. Essig, S.C. Kandarian, Activation of an alternative NF-kappaB pathway in skeletal muscle during disuse atrophy. FASEB J 16(6), 529–538 (2002)PubMedCrossRef
6.
L.G. Karagounis, B.B. Yaspelkis 3rd, D.W. Reeder, G.I. Lancaster, J.A. Hawley, V.G. Coffey, Contraction-induced changes in TNF alpha and Akt mediated signalling are associated with increased myofibrillar protein in rat skeletal muscle. Eur. J. Appl. Physiol. 109(5), 48–839 (2010)CrossRef
7.
L.S. Quinn, B.G. Anderson, R.H. Drivdahl, B. Alvarez, J.M. Argilés, Overexpression of interleukin15 induces skeletal muscle hypertrophy in vitro: implications for treatment of muscle wasting disorders. Exp. Cell Res. 280(1), 55–63 (2002)PubMedCrossRef
8.
F.S. Lira, C.H. Koyama, A.S. Yamashita, J.C. Rosa, N.E. Zanchi, M.L. Batista Jr et al., Chronic exercise decreases cytokines production in healthy rat skeletal muscle. Cell Biochem. Funct. 27, 458–461 (2009)PubMedCrossRef
9.
N.E. Zanchi, F.S. Lira, M.A. de Siqueira Filho, J.C. Rosa, C.R. de Oliveira Carvalho, M. Seelaender et al., Chronic low frequency/low volume resistance training reduces pro-inflammatory cytokine protein levels and TLR4 mRNA in rat skeletal muscle. Eur. J. Appl. Physiol. 109(6), 1095–1102 (2010)PubMedCrossRef
10.
S.C. Forbes, J.P. Little, D.G. Candow, Exercise and nutritional interventions for improving aging muscle health. Endocrine 42(1), 29–38 (2012)PubMedCrossRef
11.
B.K. Pedersen, M.A. Febbraio, Muscle as an endocrine organ: focus on muscle-derived interleukin-6. Physiol. Rev. 88(4), 1379–1406 (2008)PubMedCrossRef
12.
F. Haddad, G.R. Adams, Selected contribution: acute cellular and molecular responses to resistance exercise. J. Appl. Physiol. 93, 394–403 (2002)PubMed
13.
T.O. Takala, P. Nuutila, J. Knuuti, M. Luotolahti, H. Yki-Järvinen, Insulin action on heart and skeletal muscle glucose uptake in weight lifters and endurance athletes. Am. J. Physiol. 276(4 Pt 1), E706–E711 (1999)PubMed
14.
J.O. Holloszy, Exercise-induced increase in muscle insulin sensitivity. J. Appl. Physiol. 99(1), 338–343 (2005)PubMedCrossRef
15.
A.L. Carey, G.R. Steinberg, S.L. Macaulay, W.G. Thomas, A.G. Holmes, G. Ramm et al., Interleukin-6 increases insulin-stimulated glucose disposal in humans and glucose uptake and fatty acidoxidation in vitro via AMP-activated protein kinase. Diabetes 55(10), 2688–2697 (2006)PubMedCrossRef
16.
K.H. Grabstein, J. Eisenman, K. Shanebeck, C. Rauch, S. Srinivasan, V. Fung et al., Cloning of a T cell growth factor that interacts with the beta chain of the interleukin-2 receptor. Science 264(5161), 965–968 (1994)PubMedCrossRef
17.
H.C. Kim, H.Y. Cho, Y.S. Hah, Role of IL-15 in sepsis-induced skeletal muscle atrophy and proteolysis. Tuberc. Respir. Dis. (Seoul) 73(6), 312–319 (2012)PubMedCentralCrossRef
18.
M. Figueras, S. Busquets, N. Carbó, E. Barreiro, V. Almendro, J.M. Argilés et al., Interleukin 15 is able to suppress the increased DNA fragmentation associated with musclewasting in tumour-bearing rats. FEBS Lett 569(1–3), 201–206 (2004)PubMedCrossRef
19.
J. Xia, W. Liu, B. Hu, Z. Tian, Y. Yang, IL-15promotes regulatory T cell function and protects against diabetes development in NK-depleted NOD mice. Clin Immunol 134(2), 130–139 (2010)PubMedCrossRef
20.
S. Kuczyński, H. Winiarska, M. Abramczyk, K. Szczawińska, B. Wierusz-Wysocka, M. Dworacka, IL-15 is elevated in serum patients with type 1 diabetes mellitus. Diabetes Res. Clin. Pract. 69(3), 231–236 (2005)PubMedCrossRef
21.
S.R. Gray, T. Kamolrat, The effect of exercise induced cytokines on insulin stimulated glucose transport in C2C12 cells. Cytokine 55(2), 221–228 (2011)PubMedCrossRef
22.
S.E. Riechman, G. Balasekaran, S.M. Roth, R.E. Ferrell, Association of interleukin-15 protein and interleukin-15 receptor genetic variation with resistance exercise training responses. J. Appl. Physiol. 97, 2214–2219 (2004)PubMedCrossRef
23.
A.R. Nielsen, R. Mounier, P. Plomgaard, O.H. Mortensen, M. Penkowa, T. Speerschneider et al., Expression of interleukin-15 in human skeletal muscle effect of exercise and muscle fibre type composition. J. Physiol. 584(Pt 1), 305–312 (2007)PubMedCentralPubMedCrossRef
24.
L.S. Quinn, B.G. Anderson, L. Strait-Bodey, T. Wolden-Hanson, Serum and muscle interleukin-15 levels decrease in aging mice: correlation with declines in soluble interleukin-15 receptor alpha expression. Exp. Gerontol. 45(2), 106–112 (2010)PubMedCentralPubMedCrossRef
25.
E. Marzetti, C.S. Carter, S.E. Wohlgemuth, H.A. Lees, S. Giovannini, B. Anderson et al., Changes in IL-15 expression and death-receptor apoptotic signaling in rat gastrocnemius muscle with aging and life-long calorie restriction. Mech. Ageing Dev. 130(4), 272–280 (2009)PubMedCentralPubMedCrossRef
26.
E.E. Pistilli, P.M. Siu, S.E. Alway, Interleukin-15 responses to aging and unloading-induced skeletal muscle atrophy. Am. J. Physiol. Cell Physiol. 292(4), C1298–C1304 (2007)PubMedCrossRef
27.
Y. Tamura, K. Watanabe, T. Kantani, J. Hyashi, N. Ishida, M. Kaneki, Upregulation of circulating IL-15 by treadmill running in healthy individuals: is IL-15 an endocrine mediator of the beneficial effects of endurance exercise? Endocr. J. 58, 211–215 (2011)PubMedCrossRef
28.
S. Lee, E.R. Barton, H.L. Sweeney, R.P. Farrar, Viral expression of insulin-like growth factor-I enhances muscle hypertrophy in resistance-trained rats. J. Appl. Physiol. 96(3), 1097–1104 (2004)PubMedCrossRef
29.
P.A. Farrell, M.J. Fedele, J. Hernandez, J.D. Fluckey, J.L. Miller 3rd, C.H. Lang et al., Hypertrophy of skeletal muscle in diabetic rats in response to chronic resistance exercise. J. Appl. Physiol. 87(3), 1075–1082 (1999)PubMed
30.
E. Talebi-Garakani, A. Safarzade, Resistance training decreases serum inflammatory markers in diabetic rats. Endocrine 43(3), 564–570 (2013)PubMedCrossRef
31.
K.J. Livak, T.D. Schmittgen, Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25(4), 402–408 (2001)PubMedCrossRef
32.
M.M. Bradford, A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254 (1976)PubMedCrossRef
33.
T.A. Hornberger Jr, R.P. Farrar, Physiological hypertrophy of the FHL muscle following 8 weeks of progressive resistance exercise in the rat. Can. J. Appl. Physiol. 29(1), 16–31 (2004)PubMedCrossRef
34.
H. Yang, J. Chang, W. Chen, L. Zhao, B. Qu, C. Tang et al., Treadmill exercise promotes interleukin 15 expression in skeletal muscle and interleukin 15 receptor alphaexpression in adipose tissue of high-fat diet rats. Endocrine 43(3), 579–585 (2013)PubMedCrossRef
35.
T.A. Fehniger, M.A. Caligiuri, Interleukin 15: biology and relevance to human disease. Blood 97, 14–32 (2001)PubMedCrossRef
36.
V. Budagian, E. Bulanova, R. Paus, S. Bulfone-Paus, IL-15/IL-15 receptor biology: a guided tour through an expanding universe. Cytokine Growth Factor Rev. 17(4), 259–280 (2006)PubMedCrossRef
37.
K. Baar, K. Esser, Phosphorylation of p70(S6k) correlates with increased skeletal muscle mass following resistance exercise. Am. J. Physiol. 276(1 Pt 1), C120–C127 (1999)PubMed
38.
M. Cesari, B.W. Penninx, M. Pahor, F. Lauretani, A.M. Corsi, W.G. Rhys et al., Inflammatory markers and physical performance in older persons: the In CHIANTI study. J. Gerontol. A Biol. Sci. Med. Sci. 59, 242–248 (2004)PubMedCrossRef
39.
L.H. Colbert, M. Visser, E.M. Simonsick, R.P. Tracy, A.B. Newman, S.B. Kritchevsky et al., Physical activity, exercise, inflammatory markers in older adults: findings from the health, aging and body composition study. J. Am. Geriatr. Soc. 52, 1098–1104 (2004)PubMedCrossRef
40.
N.H. Yeo, J. Woo, K.O. Shin, J.Y. Park, S. Kang, The effects of different exercise intensity on myokine and angiogenesis factors. J. Sport. Med. Phys. Fit. 52(4), 448–454 (2012)
41.
42.
N.B. Abdulrazaq, M.M. Cho, N.N. Win, R. Zaman, M.T. Rahman, Beneficial effects of ginger (Zingiber officinale) on carbohydrate metabolism in streptozotocin-induced diabetic rats. Br. J. Nutr. 12, 1–8 (2011)
43.
O. Bozkurt, M. Severcan, F. Severcan, Diabetes induces compositional, structural and functional alterations on rat skeletal soleus muscle revealed by FTIR spectroscopy: a comparative study with EDL muscle. Analyst 135(12), 3110–3119 (2010)PubMedCrossRef
44.
J.F. Liu, W.Y. Chang, K.H. Chan, W.Y. Tsai, C.L. Lin, M.C. Hsu, Blood lipid peroxides and muscle damage increased following intensive resistance training of female weightlifters. Ann. N. Y. Acad. Sci. 1042, 255–261 (2005)PubMedCrossRef
45.
C. Scheele, S. Nielsen, B.K. Pedersen, ROS and myokines promote muscle adaptation to exercise. Trends Endocrinol. Metab. 20(3), 95–99 (2009)PubMedCrossRef
46.
S. Copray, R. Liem, N. Brouwer, P. Greenhaff, F. Habens, P. Fernyhough, Contraction-induced muscle fiber damage is increased in soleus muscle of streptozotocin-diabetic rats and is associateed with elevated expression of brain derived neurotrophic factor mRNA in muscle fibers and activated satellite cells. Exp. Neurol. 161(2), 597–608 (2000)PubMedCrossRef
47.
A. Rabinovitch, W.L. Suarez-Pinzon, Cytokines and their roles in pancreatic islet h-cell destruction and insulin-dependent diabetes mellitus. Biochem. Pharmacol. 55, 1139–1149 (1998)PubMedCrossRef
48.
S.K. Jain, K. Kannan, G. Lim, R. McVie, J.A. Bocchini, Hyperketonemia increases TNF-α secretion in cultured U937 monocytes and type-1 diabetic patients. Diabetes 51, 2287–2293 (2002)PubMedCrossRef
49.
M.G. Cavallo, P. Pozzilli, C. Bird, M. Wadhwa, A. Meager, N. Visalli et al., Cytokines in sera from insulin dependent diabetic patients at diagnosis. Clin. Exp. Immunol. 86, 256–259 (1991)PubMedCentralPubMedCrossRef
50.
R.A. Frost, G.J. Nystrom, C.H. Lang, Lipopolysaccharide and proinflammatory cytokines stimulate interleukin-6 expression in C2C12 myoblasts: role of the Jun NH2-terminal kinase. Am. J. Physiol. Regul. Integr. Comp. Physiol. 285, R1153–R1164 (2003)PubMed
51.
H. Klitgaard, A model for quantitative strength training of hindlimb muscles of the rat. J. Appl. Physiol. 64(4), 1740–1745 (1988)PubMed
52.
G. Whyte, The Physiology of Training (Advances in Sport and Exercise Science) (Amazon, 2006), pp. 135–161
53.
R.S. Staron, R.S. Hikida, T.F. Murray, M.M. Nelson, P. Johnson, F.C. Hagerman, Assessment of skeletal muscle damage in successive biopsies from strength-trained and untrained men and women. Eur. J. Appl. Physiol. 65, 258–264 (1992)CrossRef
54.
S.M. Roth, G.F. Martel, F.M. Ivey, J.T. Lemmer, E.J. Metter, B.F. Hurley, M.A. Rogers, High-volume, heavy-resistance strength training and muscle damage in young and older women. J. Appl. Physiol. 88(3), 1112–1118 (2000)PubMed
55.
N.E. Zanchi, F.S. Lira, M. Seelaender, A.H. Lancha-Jr, Experimental chronic low-frequency resistance training produces skeletal muscle hypertrophy in the absence of muscle damage and metabolic stress markers. Cell Biochem. Funct. 28(3), 232–238 (2010)PubMedCrossRef
56.
D. Hansen, B.O. Eijnde, M. Roelants, T. Broekmans, J.L. Rummens, K. Hensen et al., Clinical benefits of the addition of lower extremity low-intensity resistance muscle training to early aerobic endurance training intervention in patients with coronary artery disease: a randomized controlled trial. J. Rehabil. Med. 43(9), 800–807 (2011)PubMedCrossRef
57.
S.L. Lee, K.W. Chen, S.T. Chen, P.J. Chu, C.S. Chen, M.C. Hsu et al., Effect of passive repetitive isokinetic training on cytokines and hormonal changes. Chin. J. Physiol. 54(1), 55–66 (2011)PubMedCrossRef
58.
A.L. Mackey, M. Kjaer, S. Dandanell, K.H. Mikkelsen, L. Holm, S. Dossing et al., The influence of anti-inflammatory medication on exercise-induced myogenic precursor cell responses in humans. J. Appl. Physiol. 103, 425–431 (2007)PubMedCrossRef
59.
Q.A. Soltow, J.L. Betters, J.E. Sellman, V.A. Lira, J.H. Long, D.S. Criswell, Ibuprofen inhibits skeletal muscle hypertrophy in rats. Med. Sci. Sport. Exerc. 38, 840–846 (2006)CrossRef
Metadaten
Titel
Expression of interleukin-15 and inflammatory cytokines in skeletal muscles of STZ-induced diabetic rats: effect of resistance exercise training
verfasst von
M. Molanouri Shamsi
Z. H. Hassan
R. Gharakhanlou
L. S. Quinn
K. Azadmanesh
L. Baghersad
A. Isanejad
M. Mahdavi
Publikationsdatum
01.05.2014
Verlag
Springer US
Erschienen in
Endocrine / Ausgabe 1/2014
Print ISSN: 1355-008X
Elektronische ISSN: 1559-0100
DOI
https://doi.org/10.1007/s12020-013-0038-4

Weitere Artikel der Ausgabe 1/2014

Endocrine 1/2014 Zur Ausgabe

Original Article

The clinical and pathological significance of nitric oxide synthase in human pituitary adenomas: a comparison with MIB-1

Research Letter

Papillary carcinoma in thyroglossal duct cyst: role of fine needle aspiration and frozen section biopsy to guide surgical approach

Editorial

SMART diabetes: the way to go (Safe and Multifactorial Approach to reduce the Risk for Therapy in diabetes)

Letter to the Editor

Serum ischaemic-modified albumin levels might not be a marker of oxidative stress in patients with hypothyroidism

Original Article

Moderate to severe nausea in radioactive iodine (RAI) therapy is associated with the RAI dose per body weight and was not prevented by ramosetron

Letter to the Editor

Author reply to: serum ischaemic-modified albumin levels might not be a marker of oxidative stress in patients with hypothyroidism

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

Notfall-TEP der Hüfte ist auch bei 90-Jährigen machbar

26.04.2024 Hüft-TEP Nachrichten

Ob bei einer Notfalloperation nach Schenkelhalsfraktur eine Hemiarthroplastik oder eine totale Endoprothese (TEP) eingebaut wird, sollte nicht allein vom Alter der Patientinnen und Patienten abhängen. Auch über 90-Jährige können von der TEP profitieren.

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

25.04.2024 Hypotonie Nachrichten

Wenn unter einer medikamentösen Hochdrucktherapie der diastolische Blutdruck in den Keller geht, steigt das Risiko für schwere kardiovaskuläre Ereignisse: Darauf deutet eine Sekundäranalyse der SPRINT-Studie hin.

Bei schweren Reaktionen auf Insektenstiche empfiehlt sich eine spezifische Immuntherapie

25.04.2024 Allergien und Intoleranzreaktionen Nachrichten

Insektenstiche sind bei Erwachsenen die häufigsten Auslöser einer Anaphylaxie. Einen wirksamen Schutz vor schweren anaphylaktischen Reaktionen bietet die allergenspezifische Immuntherapie. Jedoch kommt sie noch viel zu selten zum Einsatz.

Therapiestart mit Blutdrucksenkern erhöht Frakturrisiko

25.04.2024 Hypertonie Nachrichten

Beginnen ältere Männer im Pflegeheim eine Antihypertensiva-Therapie, dann ist die Frakturrate in den folgenden 30 Tagen mehr als verdoppelt. Besonders häufig stürzen Demenzkranke und Männer, die erstmals Blutdrucksenker nehmen. Dafür spricht eine Analyse unter US-Veteranen.

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen
Bildnachweise