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01.10.2014 | Research

Oxidative DNA Damage as a Potential Early Biomarker of Helicobacter pylori Associated Carcinogenesis

verfasst von: Yasir Raza, Adnan Khan, Amber Farooqui, Muhammad Mubarak, Alex Facista, Syed Shakeel Akhtar, Saeed Khan, Javed Iqbal Kazi, Carol Bernstein, Shahana Urooj Kazmi

Erschienen in: Pathology & Oncology Research | Ausgabe 4/2014

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Abstract

Helicobacter pylori infection is an established risk factor for gastritis, gastric ulcer, peptic ulcer and gastric cancer. CagA +ve H. pylori has been associated with oxidative DNA damage of gastric mucosa but their combined role in the development of gastric cancer is still unknown. Here we compare the combined expression of cagA and 8-hydroxy-2′-deoxyguanosine (8-OHdG) in normal, gastritis and gastric cancer tissues. Two hundred gastric biopsies from patients with dyspeptic symptoms, 70 gastric cancer tissue samples and 30 gastric biopsies from non-dyspeptic individuals (controls) were included in this study and 8-OHdG was detected by immunohistochemistry (IHC). Histological features and the presence of H. pylori infection were demonstrated by Hematoxylin and Eosin (HE), Giemsa and alcian blue-periodic acid-Schiff ± diastase (AB-PAS ± D) staining. DNA was extracted from tissues and polymerase chain reaction (PCR) performed to determine the presence of ureaseA and cagA genes of H. pylori. The results showed the presence of H. pylori in 106 (53 %) gastric biopsies out of 200 dyspeptic patients, including 70 (66 %) cases of cagA + ve H. pylori. The presence of cagA gene and high expression of 8-OHdG was highly correlated with severe gastric inflammation and gastric cancer particularly, in cases with infiltration of chronic inflammatory cells (36.8 % cagA + ve, 18 %), neutrophilic activity (47.2 %, 25.5 %), intestinal metaplasia (77.7 %, 35.7 %) and intestinal type gastric cancer (95 %, 95.4 %) (p ≤ 0.01). In conclusion, H. Pylori cagA gene expression and the detection of 8-OHdG adducts in gastric epithelium can serve as potential early biomarkers of H. Pylori-associated gastric carcinogenesis.

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Titel: Oxidative DNA Damage as a Potential Early Biomarker of Helicobacter pylori Associated Carcinogenesis
verfasst von: Yasir Raza
Adnan Khan
Amber Farooqui
Muhammad Mubarak
Alex Facista
Syed Shakeel Akhtar
Saeed Khan
Javed Iqbal Kazi
Carol Bernstein
Shahana Urooj Kazmi
Publikationsdatum: 01.10.2014
Verlag: Springer Netherlands
Erschienen in: Pathology & Oncology Research / Ausgabe 4/2014
Print ISSN: 1219-4956
Elektronische ISSN: 1532-2807
DOI: https://doi.org/10.1007/s12253-014-9762-1

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Oxidative DNA Damage as a Potential Early Biomarker of Helicobacter pylori Associated Carcinogenesis

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