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Breast Cancer

Erschienen in:

01.09.2014 | Original Article

Gene delivery of cyclin-dependent kinase inhibitors p21 ^Waf1 and p27 ^Kip1 suppresses proliferation of MCF-7 breast cancer cells in vitro

verfasst von: Dandan Jiang, Xingang Wang, Xiangping Liu, Funian Li

Erschienen in: Breast Cancer | Ausgabe 5/2014

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Abstract

Background

Because tumorigenesis depends on a variety of oncogenes, symphyseal study of combined genes may lead to more significant knowledge about tumorigenesis and progression. Combined deficiency of p21 and p27 proteins in mice is linked to more aggressive spontaneous tumorigenesis. We investigated the effect of the transfected p21 ^Waf1-p27 ^Kip1 gene on centrosome duplication, cell proliferation, and apoptosis of MCF-7, a breast cancer cell line.

Methods

The pIRES-p21 ^Waf1, pIRES-p27 ^Kip1, and pIRES-p21 ^Waf1-p27 ^Kip1 genes were transfected into MCF-7 cells by lipofection. The effect on proliferation was evaluated by MTT assay and clone-formation assay. Cell cycle and apoptosis were analyzed by flow cytometry. Apoptosis was tested by flow cytometry and TUNEL assay. Centrosome duplication was detected by use of indirect immunofluorescence microscopy.

Results

The results showed that the pIRES-p21 ^Waf1, pIRES-p27 ^Kip1, and pIRES-p21 ^Waf1-p27 ^Kip1 significantly inhibited proliferation of MCF-7 cells, followed by accumulation of MCF-7 cells in cycle G₁, induced apoptosis, and a decrease in the proportion of MCF-7 cells which contained abnormal centrosomes. Compared with p21 ^Waf1 or p27 ^Kip1 alone, combination of p21 ^Waf1 and p27 ^Kip1 had a much more significant effect (P < 0.05).

Conclusion

Altogether, these results indicate that the p21 ^Waf1-p27 ^Kip1 gene combination has a more obvious antitumor effect than p21 ^Waf1 or p27 ^Kip1 alone. This study provides preclinical evidence that combination of p21 ^Waf1 and p27 ^Kip1 could be a novel and promising therapeutic approach to treatment of breast cancer with suppressed p21 ^Waf1 and p27 ^Kip1 expression.

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Titel: Gene delivery of cyclin-dependent kinase inhibitors p21 Waf1 and p27 Kip1 suppresses proliferation of MCF-7 breast cancer cells in vitro
verfasst von: Dandan Jiang
Xingang Wang
Xiangping Liu
Funian Li
Publikationsdatum: 01.09.2014
Verlag: Springer Japan
Erschienen in: Breast Cancer / Ausgabe 5/2014
Print ISSN: 1340-6868
Elektronische ISSN: 1880-4233
DOI: https://doi.org/10.1007/s12282-012-0438-y

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Springer Medizin

Gene delivery of cyclin-dependent kinase inhibitors p21 ^Waf1 and p27 ^Kip1 suppresses proliferation of MCF-7 breast cancer cells in vitro

Abstract

Background

Methods

Results

Conclusion

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Springer Medizin

Abstract

Background

Methods

Results

Conclusion

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