Introduction
Methods
Participants
Testing sessions
Strength assessment
Body composition assessment
Venous blood sampling and percutaneous muscle biopsies
Supplementation protocol
Dietary monitoring
Resistance-training protocol
Whole blood and serum clinical chemistry analyses
Serum IGF-1 analysis
Skeletal muscle cellular extraction
Myogenic regulatory factor quantitation
Myofibrillar protein content
Myosin heavy chain isoform protein quantitation
Reported side effects from supplements
Statistical analysis
Results
Subject demographics
Dietary analysis, supplement compliance, and reported side effects
Variable | Group | Day 0 | Day 29 | Test (p < .05) | Group x Test (p < .05) |
---|---|---|---|---|---|
Total Calories (kcal/kg)
| .683 | .129 | |||
NOSS
| 30.12 ± 9.94 | 31.61 ± 10.58 | |||
CARB
| 41.81 ± 18.98 | 35.41 ± 16.16 | |||
Protein (g/kg)
| .763 | .216 | |||
NOSS
| 1.17 ± 0.33 | 1.25 ± 0.42 | |||
CARB
| 1.57 ± 0.77 | 1.31 ± 0.43 | |||
Carbohydrate (g/kg)
| .932 | .106 | |||
NOSS
| 3.64 ± 1.39 | 4.01 ± 1.41 | |||
CARB
| 5.03 2.82 | 4.13 2.22 | |||
Fat (g/kg)
| .551 | .665 | |||
NOSS
| 1.17 ± 0.41 | 1.15 ± 0.44 | |||
CARB
| 1.47 ± 0.64 | 1.27 ± 0.77 |
Body composition
Variable | Group | Day 0 | Day 29 | Test (p < .05) | Group x Test (p < .05) |
---|---|---|---|---|---|
Body Mass (kg)
| .010* | .793 | |||
NOSS
| 81.41 ± 16.46 | 82.64 ± 15.97 | |||
CARB
| 84.41 ± 29.39 | 85.44 ± 29.32 | |||
Body Water (kg)
| .345 | .587 | |||
NOSS
| 43.07 ± 10.33 | 43.43 ± 9.68 | |||
CARB
| 43.18 ± 6.50 | 44.12 ± 7.13 | |||
Body Fat (%)
| .026* | .014† NOSS < PLC | |||
NOSS
| 17.88 ± 7.67 | 16.53 ± 7.35 | |||
CARB
| 21.18 ± 9.06 | 21.26 ± 9.55 | |||
Fat Mass (kg)
| .046* | .026† NOSS < PLC | |||
NOSS
| 14.21 ± 8.31 | 13.40 ± 8.10 | |||
CARB
| 18.64 ± 18.16 | 18.99 ± 18.64 | |||
Fat-Free Mass (kg)
| .001* | .023† NOSS > PLC | |||
NOSS
| 57.80 ± 8.01 | 59.92 ± 7.57 | |||
CARB
| 56.43 ± 10.33 | 57.02 ± 9.86 | |||
Upper-Body Strength (kg/kg)
| .016* | .023† NOSS > PLAC | |||
NOSS
| 1.03 ±0.15 | 1.16 ±0.21 | |||
CARB
| 1.07 ±0.25 | 1.08 ± 0.23 | |||
Lower-Body Strength (kg/kg)
| .001* | .035† NOSS > PLAC | |||
NOSS
| 4.04 ± 0.55 | 4.90 ± 0.64 | |||
CARB
| 4.19 ± 0.58 | 4.64 ± 0.84 |
Muscle strength
Serum IGF-1
Variable | Group | Day 0 | Day 29 | Test (p < .05) | Group x Test (p < .05) |
---|---|---|---|---|---|
IGF-1 (pg/ml)
| .038* | .385 | |||
NOSS
| 3491.53 ± 597.34 | 3609.63 ± 497.11 | |||
CARB
| 3018.43 ± 690.91 | 3339.24 ± 70.94 | |||
Myofibrillar Protein (μg/mg)
| .041* | .049† NOSS > PLC | |||
NOSS
| 0.089 ± 0.019 | 0.115 ± 0.033 | |||
CARB
| 0.087 ± 0.128 | 0.092 ± 0.022 | |||
MHC 1 (arbitrary density units)
| .001* | .013† NOSS > PLC | |||
NOSS
| 1072.93 ± 206.16 | 1582.37 ± 247.55 | |||
CARB
| 1114.95 ± 448.29 | 1381.76 ± 423.04 | |||
MHC 2A (arbitrary density units)
| .001* | .046† NOSS > PLC | |||
NOSS
| 904.06 ± 500.22 | 1502.84 ± 412.07 | |||
CARB
| 944.11 ± 458.98 | 1385.97 ± 310.87 | |||
MHC 2X (arbitrary density units)
| .003* | .244 | |||
NOSS
| 878.45 ± 328.28 | 731.70 ± 266.26 | |||
CARB
| 979.89 ± 226.74 | 676.78 ± 163.27 | |||
Myo-D (Abs/mg)
| .005* | .038† NOSS > PLC | |||
NOSS
| 1.72 ± 0.491 | 2.03 ± 0.399 | |||
CARB
| 1.65 ± 0.339 | 1.74 ± 0.462 | |||
Myogenin (Abs/mg)
| .017* | .091 | |||
NOSS
| 1.63 ± 0.398 | 1.85 ± 0.422 | |||
CARB
| 1.57 ± 0.240 | 1.78 ± 0.405 | |||
MRF-4 (Abs/mg)
| .001* | .001† NOSS > PLC | |||
NOSS
| 1.87 ± 0.236 | 2.25 ± 0.247 | |||
CARB
| 1.83 ± 0.005 | 1.97 ± 0.003 |
Myogenic regulatory factors
Myofibrillar protein and MHC isoforms
Serum and whole blood clinical chemistry markers
Variable | Group | Day 0 | Day 29 | Test (p < .05) | Group x Test (p < .05) |
---|---|---|---|---|---|
Glucose (mg/dL)
| .142 | .315 | |||
NOSS
| 82.00 ± 4.98 | 83.00 ± 11.45 | |||
CARB
| 81.80 ± 6.52 | 86.90 ± 5.56 | |||
BUN (mg/dL)
| .117 | .117 | |||
NOSS
| 14.80 ± 3.42 | 14.80 ± 3.22 | |||
CARB
| 15.33 ± 2.82 | 12.88 ± 3.85 | |||
Creatinine (mg/dL)
| .016* | .413 | |||
NOSS
| 0.896 ± 0.150 | 0.949 ± 0.145 | |||
CARB
| 0.916 ± 0.087 | 1.07 ± 0.201 | |||
Sodium (mmol/L)
| .304 | .681 | |||
NOSS
| 137.40 ± 2.79 | 139.72 ± 2.26 | |||
CARB
| 136.67 ± 2.06 | 137.67 ± 9.08 | |||
Potassium (mmol/L)
| .107 | .671 | |||
NOSS
| 4.06 ± 0.177 | 4.34 ± 0.568 | |||
CARB
| 3.95 ± 0.274 | 4.12 ± 0.446 | |||
Chloride (mmol/L)
| .665 | .561 | |||
NOSS
| 101.70 ± 2.79 | 103.24 ± 2.85 | |||
CARB
| 101.33 ± 2.64 | 101.11 ± 8.47 | |||
CO
2
(mmol/L)
| .787 | .349 | |||
NOSS
| 21.40 ± 1.07 | 22.40 ± 1.07 | |||
CARB
| 20.11 ± 2.20 | 19.55 ± 4.90 | |||
Calcium (mg/dL)
| .799 | .247 | |||
NOSS
| 9.27 ± 0.577 | 9.58 ± 0.322 | |||
CARB
| 9.28 ± 0.481 | 9.07 ± 1.24 | |||
Protein (mg/dL)
| .914 | .336 | |||
NOSS
| 6.79 ± 0.645 | 6.95 ± 0.353 | |||
CARB
| 6.85 ± 0.657 | 6.65 ± 1.18 | |||
Albumin (mg/dL)
| .653 | .244 | |||
NOSS
| 4.41 ± 0.338 | 4.50 ± 0.205 | |||
CARB
| 4.48 ± 0.341 | 4.28 ± 0.695 | |||
Globulin (mg/dL)
| .622 | .622 | |||
NOSS
| 2.38 ± 0.410 | 2.45 ± 0.310 | |||
CARB
| 2.36 ± 0.393 | 2.36 ± 0.574 | |||
Albumin/Globulin
| .157 | .436 | |||
NOSS
| 1.89 ± 0.237 | 1.87 ± 0.231 | |||
CARB
| 1.93 ± 0.300 | 1.86 ± 0.304 | |||
Bilirubin (mg/dL)
| .181 | .465 | |||
NOSS
| 0.550 ± 0.295 | 0.440 ± 0.171 | |||
CARB
| 0.667 ± 0.282 | 0.633 ± 0.400 | |||
ALP (U/L)
| .066 | .816 | |||
NOSS
| 52.30 ± 12.84 | 56.40 ± 18.36 | |||
CARB
| 58.55 ± 15.56 | 63.77 ± 21.77 | |||
AST (U/L)
| .982 | .403 | |||
NOSS
| 17.80 ± 4.15 | 17.40 ± 4.67 | |||
CARB
| 17.55 ± 5.07 | 17.88 ± 4.59 | |||
ALT (U/L)
| .785 | .785 | |||
NOSS
| 10.70 ± 4.16 | 10.70 ± 6.26 | |||
CARB
| 7.66 ± 1.93 | 8.33 ± 5.63 |
Variable | Group | Day 0 | Day 29 | Test (p < .05) | Group x Test (p < .05) |
---|---|---|---|---|---|
WBC (10
6
/L)
| .148 | .300 | |||
NOSS
| 6.06 ± 1.32 | 5.91 ± 1.27 | |||
CARB
| 7.11 ± 1.74 | 6.28 ± 1.34 | |||
RBC (10
9
/L)
| .243 | .755 | |||
NOSS
| 4.99 ± 0.304 | 4.94 ± 0.257 | |||
CARB
| 4.94 ± 0.312 | 4.85 ± 0.302 | |||
HGB (g/dL)
| .100 | .695 | |||
NOSS
| 15.24 ± 0.815 | 15.01 ± 0.750 | |||
CARB
| 14.96 ± 0.593 | 14.60 ± 0.563 | |||
HCT (%)
| .509 | .997 | |||
NOSS
| 44.28 ± 2.12 | 43.92 ± 2.34 | |||
CARB
| 43.44 ± 1.81 | 43.08 ± 2.03 | |||
MCV (fL)
| .093 | .226 | |||
NOSS
| 88.85 ± 3.90 | 88.90 ± 4.06 | |||
CARB
| 88.05 ± 4.00 | 88.86 ± 4.05 | |||
MCH (pg)
| .103 | .886 | |||
NOSS
| 30.57 ± 1.31 | 30.42 ± 1.34 | |||
CARB
| 30.32 ± 1.85 | 30.14 ± 1.62 | |||
MCHC (g/dL)
| .015 | .235 | |||
NOSS
| 34.42 ± 0.399 | 34.22 ± 0.482 | |||
CARB
| 34.45 ± 1.01 | 33.92 ± 1.03 | |||
Platelets (10
6
/L)
| .243 | .861 | |||
NOSS
| 211.24 ± 28.57 | 221.82 ± 38.27 | |||
CARB
| 228.89 ± 62.43 | 236.78 ± 77.51 | |||
Neutrophils (cells/μl)
| .178 | .111 | |||
NOSS
| 2798.63 ± 1056.71 | 2868.65 ± 886.18 | |||
CARB
| 4038.93 ± 1448.59 | 3263.78 ± 767.91 | |||
Lymphocytes (cells/μl)
| .445 | .566 | |||
NOSS
| 2512.93 ± 516.94 | 2344.63 ± 355.10 | |||
CARB
| 2164.57 ± 620.78 | 2139.84 ± 629.11 | |||
Monocytes (cells/μl)
| .613 | .888 | |||
NOSS
| 536.70 ± 121.60 | 509.40 ± 124.70 | |||
CARB
| 599.11 ± 142.53 | 583.67 ± 263.01 | |||
Eosinophils (cells/μl)
| .602 | .926 | |||
NOSS
| 186.40 ± 113.01 | 177.10 ± 143.54 | |||
CARB
| 287.33 ± 161.21 | 274.03 ± 183.27 | |||
Basophils (cells/μl)
| .293 | .050† NOSS < PLC | |||
NOSS
| 25.70 ± 10.34 | 17.40 ± 8.51 | |||
CARB
| 21.88 ± 11.43 | 24.55 ± 11.10 |