Background
Methods
Study design and participants
Oral glucose tolerance test (OGTT)
Insulin sensitivity, insulin secretion, and analytical methods
Determination of body and liver fat
Genotyping
Statistics
Results
SIRT1genetic variants in the TUEF/TULIP cohort
Cross-sectional TUEF study
SNP rs12413112 | Genotype | Calculation Model | ||||
---|---|---|---|---|---|---|
GG | GA | AA | padd
| pdom
| prec
| |
n | 726 | 173 | 18 | |||
Sex (men/women), n
| 266/460 | 72/101 | 6/12 | 0.45a
| 0.29 | 0.71 |
Age (years) | 38.6 ± 12.5 | 38.9 ± 12.8 | 37.9 ± 13.9 | 0.92 | 0.86 | 0.71 |
Waist-to-hip ratio | 0.87 ± 0.09 | 0.87 ± 0.09 | 0.86 ± 0.12 | 0.69b
| 0.39 | 0.80 |
BMI (kg/m2) | 29.3 ± 8.8 | 29.1 ± 8.0 | 29.7 ± 8.7 | 0.08 | 0.46 |
0.02
|
Body fat (%) | 30.4 ± 10.9 | 30.1 ± 11.2 | 34.0 ± 10.7 | 0.29 | 0.53 | 0.12 |
Fasting glucose (mmol/l) | 5.12 ± 0.66 | 5.13 ± 0.62 | 5.07 ± 0.55 | 0.87c
| 0.83 | 0.61 |
2 h glucose (mmol/l) | 6.27 ± 1.73 | 6.42 ± 1.62 | 5.84 ± 1.79 | 0.07 | 0.42 | 0.08 |
Fasting insulin (pmol/l) | 64.8 ± 55.1 | 61.6 ± 47.0 | 63.5 ± 57.5 | 0.52 | 0.35 | 0.26 |
2 h insulin (pmol/l) | 429 ± 442 | 441 ± 366 | 397 ± 337 | 0.09 | 0.42 | 0.29 |
Insulin sensitivity (AU) | 16.6 ± 11.0 | 15.8 ± 10.7 | 16.6 ± 8.9 | 0.19 | 0.90 | 0.19 |
Insulin secretion (pmol/l) | 2046 ± 898 | 2115 ± 907 | 2172 ± 1054 | 0.68 | 0.49 | 0.53 |
Basal energy exp (kcal/24 h)d
| 1730 ± 297 | 1746 ± 331 | 1655 ± 292 | 0.10 |
0.039
| 0.31 |
Respiratory quotientd
| 0.81 ± 0.07 | 0.83 ± 0.07 | 0.81 ± 0.08 |
0.048
|
0.017
| 0.92 |
rs7069102 | GG | GC | CC | |||
n | 457 | 366 | 94 | |||
Sex (men/women), n
| 172/285 | 138/228 | 34/60 | 0.96 | 0.94 | 0.78 |
Age (years) | 38.2 ± 12.2 | 39.4 ± 12.8 | 37.9 ± 13.1 | 0.36 | 0.26 | 0.48 |
Waist-to-hip ratio | 0.86 ± 0.09 | 0.87 ± 0.09 | 0.86 ± 0.10 | 0.84 | 0.92 | 0.56 |
BMI (kg/m2) | 29.5 ± 8.9 | 29.1 ± 8.7 | 28.8 ± 7.5 | 0.51 | 0.48 | 0.53 |
Body fat (%) | 30.5 ± 11.2 | 30.2 ± 10.7 | 30.6 ± 11.4 | 0.92 | 0.68 | 0.88 |
Fasting glucose (mmol/l) | 5.13 ± 0.68 | 5.12 ± 0.63 | 5.08 ± 0.55 | 0.79 | 0.64 | 0.65 |
2 h glucose (mmol/l) | 6.27 ± 1.74 | 6.30 ± 1.64 | 6.32 ± 1.86 | 0.94 | 0.89 | 0.73 |
Fasting insulin (pmol/l) | 65.2 ± 51.6 | 63.2 ± 56.6 | 62.3 ± 52.1 | 0.73 | 0.92 | 0.43 |
2 h insulin (pmol/l) | 441 ± 450 | 418 ± 401 | 432 ± 408 | 0.99 | 0.89 | 0.95 |
Insulin sensitivity (AU) | 16.7 ± 11.6 | 16.2 ± 10.4 | 16.2 ± 9.9 | 0.88 | 0.70 | 0.67 |
Insulin secretion (pmol/l) | 2091 ± 947 | 2017 ± 856 | 2097 ± 866 | 0.47 | 0.44 | 0.44 |
Basal energy exp (kcal/24 h) | 1742 ± 288 | 1734 ± 334 | 1680 ± 245 | 0.06 |
0.05
| 0.56 |
Respiratory quotient | 0.81 ± 0.07 | 0.82 ± 0.07 | 0.81 ± 0.07 | 0.35 | 0.18 | 0.90 |
rs730821 | AA | AG | GG | |||
n | 603 | 286 | 28 | |||
Sex (men/women), n
| 237/366 | 94/192 | 13/15 | 0.11 | 0.12 | 0.33 |
Age (years) | 38.4 ± 12.4 | 39.3 ± 12.9 | 37.5 ± 13.4 | 0.59 | 0.37 | 0.58 |
Waist-to-hip ratio | 0.87 ± 0.09 | 0.87 ± 0.09 | 0.86 ± 0.12 | 0.38 | 0.55 | 0.28 |
BMI (kg/m2) | 29.5 ± 8.7 | 29.0 ± 8.6 | 27.7 ± 7.5 | 0.28 | 0.12 | 0.39 |
Body fat (%) | 30.4 ± 11.2 | 30.5 ± 10.4 | 27.2 ± 11.3 | 0.23 | 0.26 | 0.13 |
Fasting glucose (mmol/l) | 5.13 ± 0.67 | 5.11 ± 0.59 | 5.01 ± 0.66 | 0.39 | 0.66 | 0.22 |
2 h glucose (mmol/l) | 6.28 ± 1.72 | 6.31 ± 1.66 | 6.11 ± 2.10 | 0.34 | 0.92 | 0.14 |
Fasting insulin (pmol/l) | 65.1 ± 51.4 | 61.7 ± 57.5 | 68.8 ± 61.8 | 0.55 | 0.92 | 0.68 |
2 h insulin (pmol/l) | 443 ± 440 | 406 ± 387 | 433 ± 527 | 0.62 | 0.69 | 0.55 |
Insulin sensitivity (AU) | 16.5 ± 11.5 | 16.2 ± 9.6 | 18.1 ± 12.3 | 0.80 | 0.82 | 0.91 |
Insulin secretion (pmol/l) | 2098 ± 945 | 1997 ± 822 | 1929 ± 694 | 0.68 | 0.38 | 0.64 |
Basal energy exp (kcal/24 h) | 1746 ± 305 | 1714 ± 306 | 1642 ± 217 | 0.89 | 0.86 | 0.63 |
Respiratory quotient | 0.82 ± 0.07 | 0.81 ± 0.07 | 0.82 ± 0.08 | 0.79 | 0.48 | 0.88 |
rs2273773 | TT | TC | CC | |||
n | 812 | 103 | 2 | |||
Sex (men/women), n
| 313/499 | 30/73 | 1/1 | 0.16 | 0.07 | 0.72 |
Age (years) | 38.7 ± 12.4 | 38.5 ± 13.6 | 33.0 ± 4.2 | 0.82 | 0.80 | 0.66 |
Waist-to-hip ratio | 0.87 ± 0.09 | 0.86 ± 0.10 | 0.77 ± 0.02 | 0.10 | 0.68 |
0.04
|
BMI (kg/m2) | 29.3 ± 8.9 | 29.2 ± 7.2 | 24.3 ± 7.1 | 0.72 | 0.99 | 0.42 |
Body fat (%) | 30.2 ± 11.1 | 31.9 ± 9.7 | 28.0 ± 18.4 | 0.78 | 0.52 | 0.84 |
Fasting glucose (mmol/l) | 5.13 ± 0.65 | 5.05 ± 0.62 | 4.96 ± 0.09 | 0.19 | 0.35 | 0.93 |
2 h glucose (mmol/l) | 6.27 ± 1.71 | 6.47 ± 1.75 | 4.58 ± 0.35 | 0.39 | 0.31 | 0.18 |
Fasting insulin (pmol/l) | 63.4 ± 50.5 | 70.2 ± 74.3 | 33.0 ± 22.6 | 0.44 | 0.61 | 0.22 |
2 h insulin (pmol/l) | 426 ± 417 | 470 ± 494 | 178 ± 212 | 0.17 | 0.71 | 0.06 |
Insulin sensitivity (AU) | 16.5 ± 11.0 | 15.9 ± 10.1 | 26.7 ± 14.1 | 0.38 | 0.82 | 0.21 |
Insulin secretion (pmol/l) | 2070 ± 908 | 2019 ± 864 | 1299 ± 41 | 0.45 | 0.34 | 0.39 |
Basal energy exp (kcal/24 h) | 1730 ± 304 | 1756 ± 310 | 1835 ± 0 | 0.33 | 0.16 | 0.51 |
Respiratory quotient | 0.82 ± 0.07 | 0.80 ± 0.08 | 0.84 ± 0 | 0.19 | 0.09 | 0.94 |