Background
Methods
Plant Materials
Extract Preparation
Total Phenolics Assay
Total Flavonoid Assay
Antioxidant Activity of P. macrocarpa Extracts
Total Antioxidant Activity Assay
Ferric Thiocyanate (FTC)
Thiobarbituric Acid (TBA)
DPPH Radical Scavenging Activity
Ferric-Reducing Antioxidant Power (FRAP) Assay
NO-Scavenging Activity
Anti-inflammatory activity
Cytotoxic Activity
Statistical analysis
Results and Discussion
Total Phenolic and Flavonoid Contents
Sample | Total phenolica (mg/g DM) | Total flavonoidb (mg/g DM) |
---|---|---|
Pericarp | 59.2 ± 0.04 | 161.3 ± 1.58 |
Mesocarp | 60.5 ± 0.17 | 131.7 ± 1.66 |
Seed | 47.7 ± 1.04 | 35.9 ± 2.47 |
Antioxidant Assay for P. macrocarpa Extracts
Total Antioxidant Activity Assay
Antioxidant Activity of P. macrocarpa Fruit
Sample | DPPH | FRAP | NO Inhibition |
---|---|---|---|
Pericarp | 142.4 ± 1.14 | 49.9 ± 1.64 | 212.3 ± 1.33 |
Mesocarp | 230.3 ± 1.75 | 76.1 ± 1.34 | 261.5 ± 1.83 |
Seed | 245.0 ± 1.94 | 150.2 ± 1.28 | > 300 |
BHT | 78.7 ± 1.01 | 24.8 ± 1.32 | 185.6 ± 1.10 |
Anti-Inflammatory Activity
Cytotoxic Activity
Sample | IC50 Values (μg/ml) | |||
---|---|---|---|---|
HT-29 | MCF-7 | HeLa | Chang Liver | |
Pericarp | 70.1 ± 1.94*** | 33.5 ± 1.74*** | 40.8 ± 2.01*** | 103.5 ± 0.65*** |
Mesocarp | 63.8 ± 0.95*** | 26.2 ± 2.01 | 37.2 ± 1.89*** | 110.7 ± 0.71*** |
Seed | 38.4 ± 0.37 | 25.5 ± 1.37 | 29.5 ± 1.09** | 67.8 ± 0.27*** |
Tamoxifen | 37.5 ± 0.84 | 24.2 ± 1.91 | 28.8 ± 1.94 | 45.6 ± 0.62 |