Background
Methods
Plant materials
Sample extraction
LC-MS/MS analysis
Cell culture
MTT assay
Real time cell growth assay
Flow cytometry analysis
Bioluminescent assay for caspase-3/7, -8 and -9 activities
Transmission electron miscroscopy
Multiple cytotoxicity assay
ROS assay
Western blot analysis
Statistical analysis
Results
LC-MS/MS analysis
Peak # | Rt (min) | MW | [M+H]+or [M-H]-
| +/- Ions | MS/MS fragments | Tentative identification | References |
---|---|---|---|---|---|---|---|
1 | 2.3 | 378 | 377 | - | 257, 257, 230, 187 | unknown | |
2 | 3.0 | 360 | 361 | + | 259, 247, 86, 58 | vernodaline | |
3 | 3.6 | 330 | 329 | - | 229, 211, 99, 83 | vernudiflorid | [24] |
4 | 3.8 | 346 | 345 | - | 273, 209, 197,163, 84, 57 | unknown | |
5 | 4.7 | 314 | 313 | - | 213, 182, 157, 131, | 12,13- dihydroxyoleic acid |
CACF inhibits cell proliferation of melanoma cells
CACF | Doxorubicin | |||
---|---|---|---|---|
IC50(mean ± SD) μg/mL | IC50(mean ± SD) μg/mL | |||
24 h
|
48 h
|
24 h
|
48 h
| |
A375
| 9.3 ± 2.5 | 8.5 ± 1.2 | 7.6 ± 0.8 | 6.2 ± 1.5 |
Primary melanocytes
| 24.3 ± 4.6 | 21.5 ± 3.1 | 8.5 ± 2.5 | 7.2 ± 2.2 |