Background
Methods
Plant material
Extract preparation
Phytochemistry
Bacterial culture
Sr. no | Strain ID | Drug susceptibility profile* |
rpoβ
Ψ
genotype
|
katG
Ψ
genotype
| inhA promoter genotype |
---|---|---|---|---|---|
1 | H37Rv | Susceptible | Wild type | Wild type | Wild type |
2 | S1 | Susceptible | Wild type | Wild type | Wild type |
3 | S2 | Susceptible | Wild type | Wild type | Wild type |
4 | S3 | Susceptible | Wild type | Wild type | Wild type |
5 | R1 | Resistant to HR | D516V mixed | S315T1/T2 | A16G mixed |
6 | R2 | Resistant to HERZ | D516V mixed | S315T1/T2 | A16G mixed |
7 | R3 | Resistant to HERZ | S531L | S315T1/T2 mixed | Wild type |
MABA
Intracellular assay
Double stimulus
Axenic assay under differential oxygen concentration
Results
Phytochemical fingerprinting
MABA
Strains | 1: 100 VMTB |
VMTB
|
A. galanga extracts | |||||
---|---|---|---|---|---|---|---|---|
Acetone (μg/ml) | Ethanolic (μg/ml) | |||||||
25 | 50 | 100 | 25 | 50 | 100 | |||
% reduction of Alamar Blue dye | ||||||||
H37Rv | 21.71 ± 2.84 | 66.05 ± 5.03 | 56.76 ± 9.51 | 25.10 ± 11.15 |
18.51 ± 3.84
| 57.27 ± 6.11 |
14.13 ± 6.46
|
12.88 ± 7.10
|
S1 | 21.09 ± 3.42 | 56.27 ± 2.30 | 53.84 ± 2.47 | 48.93 ± 11.10 |
21.16 ± 6.22
| 47.75 ± 13.04 | 35.60 ± 2.62 |
16.63 ± 4.40
|
S2 | 22.33 ± 7.00 | 59.17 ± 4.93 | 52.78 ± 6.74 | 42.90 ± 12.23 |
15.23 ± 6.17
| 41.45 ± 5.70 |
14.31 ± 6.64
|
14.87 ± 7.91
|
S3 | 19.83 ± 1.80 | 59.74 ± 10.58 | 55.58 ± 17.71 | 52.89 ± 16.92 |
18.21 ± 2.29
| 42.05 ± 6.60 | 32.15 ± 7.40 |
18.55 ± 4.40
|
R1 | 14.40 ± 5.71 | 51.08 ± 11.91 | 34.01 ± 6.32 | 25.57 ± 6.85 |
21.24 ± 5.62
| 23.39 ± 3.53 |
16.89 ± 1.10
|
13.08 ± 5.78
|
R2 | 22.11 ± 2.47 | 60.93 ± 9.00 | 53.38 ± 8.37 | 36.98 ± 8.84 |
20.04 ± 1.22
| 36.49 ± 4.06 |
19.74 ± 4.38
|
15.74 ± 3.86
|
R3 | 16.73 ± 4.14 | 50.50 ± 4.04 | 37.10 ± 5.03 | 30.48 ± 8.23 |
13.95 ± 2.06
| 42.59 ± 7.22 |
14.25 ± 3.88
|
12.25 ± 2.87
|
Intracellular assays
Sr. no. | Strains |
A. galanga extracts | |||||
---|---|---|---|---|---|---|---|
Acetone (μg/ml) | Ethanolic (μg/ml) | ||||||
25 | 100 | 25 | 100 | ||||
Single stimulus | Double stimulus | Single stimulus | Double stimulus | ||||
1 | H37Rv | 78.25 ± 6.14 | 94.58 ± 2.98 | 98.06 ± 0.24 | 78.41 ± 5.00 | 98.06 ± 0.24 | 98.06 ± 0.24 |
2 | S1 | 83.34 ± 7.76 | 99.42 ± 0.50 | 99.37 ± 0.05 | 86.20 ± 12.04 | 98.88 ± 1.92 | 99.37 ± 0.05 |
3 | S2 | 75.62 ± 2.23 | 94.47 ± 3.88 | 99.21 ± 0.02 | 73.09 ± 3.41 | 98.86 ± 0.44 | 99.21 ± 0.02 |
4 | S3 | 96.41 ± 0.76 | 97.69 ± 1.25 | 95.18 ± 1.51 | 83.11 ± 2.33 | 100 ± 0 | 95.20 ± 1.82 |
5 | R1 | 86.70 ± 5.11 | 95.00 ± 4.69 | 94.60 ± 1.30 | 82.21 ± 12.17 | 90.89 ± 6.43 | 97.74 ± 1.11 |
6 | R2 | 89.33 ± 2.67 | 98.00 ± 0.00 | 93.56 ± 2.48 | 85.33 ± 5.81 | 93.78 ± 0.20 | 99.08 ± 0.52 |
7 | R3 | 91.11 ± 1.92 | 94.36 ± 2.67 | 100 ± 0 | 57.93 ± 8.36 | 100 ± 0 | 100 ± 0 |