Introduction
Materials and methods
Tissue samples
DNA methylation analyses
Quantitative MSP
Selection criteria for the 11 gene promoters analyzed in the present study
Gene mutation status of BRAF, KRAS and TP53
Statistics
Results
MSI status of colorectal tumors
DNA promoter methylation in normal mucosa, adenomas, and carcinomas
ADAMTS1 | CDKN2A | CRABP1 | HOXA9 | MAL | MGMT | MLH1 | NR3C1 | RUNX3 | SCGB3A1 | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
M
|
U
|
M
|
U
|
M
|
U
|
M
|
U
|
M
|
U
|
M
|
U
|
M
|
U
|
M
|
U
|
M
|
U
|
M
|
U
| |
Sample type
| ||||||||||||||||||||
N1 |
0
|
21
|
0
|
21
|
0
|
21
|
4
|
17
|
1
|
20
|
2
|
19
|
0
|
21
|
0
|
21
|
0
|
1
|
1
|
20
|
N2 |
1
|
17
|
2
|
16
|
0
|
18
|
7
|
11
|
2
|
16
|
7
|
10
|
1
|
17
|
0
|
18
|
0
|
1
|
1
|
17
|
Adenoma |
23
|
40
|
10
|
53
|
7
|
53
|
22
|
40
|
45
|
18
|
23
|
39
|
0
|
63
|
2
|
61
|
4
|
4
|
4
|
59
|
Carcinomas |
36
|
15
|
17
|
35
|
25
|
25
|
12
|
38
|
41
|
9
|
21
|
31
|
11
|
41
|
13
|
37
|
16
|
9
|
9
|
40
|
MSI status
| ||||||||||||||||||||
Carcinomas
| ||||||||||||||||||||
MSI |
19
|
8
|
10
|
17
|
22
|
5
|
7
|
20
|
21
|
6
|
11
|
16
|
11
|
16
|
12
|
15
|
16
|
8
|
8
|
18
|
MSS |
17
|
7
|
7
|
18
|
3
|
20
|
5
|
18
|
20
|
2
|
10
|
15
|
0
|
25
|
1
|
22
|
0
|
1
|
1
|
22
|
P value |
NS
|
NS
|
<0.0001
|
NS
|
NS
|
NS
|
<0.0001
|
0.001
|
<0.0001
|
0.026
|