Introduction
Materials and methods
Generation of endometrial regenerative cells
Phenotypic characterization
Karyotypic analysis
Proteomic analysis
Differentiation
Adipogenic differentiation
Osteogenic differentiation
Endothelial differentiation
Neurogenic differentiation
Pulmonary epithelial differentiation
Hepatic/pancreatic differentiation
Cardiogenic and myogenic differentiation
Results
Isolation and cloning of cells
Characterization
Marker | Relevance | Expression |
---|---|---|
CD14 | Monocyte marker | Negative |
CD34 | Hematopoietic stem cell marker | Negative |
CD38 | Differentiating hematopoietic stem cell marker | Negative |
CD45 | Pan-leukocyte marker | Negative |
CD133 | Hematopoietic/angioblast marker | Negative |
STRO-1 | MSC marker | Negative |
SSEA-4 | Embryonic stem cell marker | Negative |
Nanog | Embryonic stem cell marker | Negative |
CD9 | MSC marker, associated with angiogenesis1 | Positive |
CD29 | Adhesion molecule on mesenchymal and hepatic stem cells2 | Positive |
CD59 | Complement inhibitor protein found on MSC3 and bone marrow side population CD34-stem cells4 | Positive |
CD73 | Ecto-5'-nucleotidase, involved in migration of MSC | Positive |
CD41a | Receptor for fibrinogen and vWF, found on MSC and platelets | Positive |
CD44 | Hyaluronic acid receptor found on tissue stem cells and MSC | Positive |
CD90 | Marker of T cells, hematopoietic and MSC | Positive |
CD105 | Marker of tissue and MSC | Positive |
hTERT | Telomerase reverse transcriptase | Positive |
Oct-4 | Embryonic stem cell marker | Positive |
Factor | BioE | MYZb | ERC-1 | ERC-2 |
---|---|---|---|---|
MMP3 | 05 | 0 |
106227
|
234638
|
MMP10 | 0 | 0 |
5250
|
8944
|
GM-CSF | 0 |
452
|
15630
|
972
|
PDGF-BB | 0 | 0 |
12
|
61
|
ANG-2 | 0 | 0 |
11
|
34
|