Background
Methods
ATP synthesis assay of PBMC
Statistical analysis
Results
Patients
Patients n = 15 | Controls n = 15 | |
---|---|---|
Age (y) | 35.5 ± 11.9 | 35.6 ± 14.0 |
Body mass index (kg/m2) | 23.1 ± 5.4 | 22.7 ± 4.3 |
CDC Symptom score | 59.5 ± 13.1 | 5.0 ± 4.5* |
CPET1 and CPET2
CPET1 | CPET2 | |||
---|---|---|---|---|
Patients n = 15 | Controls n = 15 | Patients n = 15 | Controls n = 15 | |
FVC (L) | 3.70 ± 0.73 | 3.71 ± 0.77 | 3.71 ± 0.73 | 3.76 ± 0.72 |
FEV 1 (L) | 2.95 ± 0.61 | 2.98 ± 0.55 | 2.94 ± 0.46 | 3.05 ± 0.53 |
Rest
| ||||
Heart rate (beats/min) | 86.5 ± 10.6 | 80.7 ± 8.6 | 87.9 ± 11.2 | 80.9 ± 8.8 |
O2 pulse (mL/beat) | 4.43 ± 0.72 | 4.73 ± 0.86 | 4.26 ± 0.76 | 4.73 ± 0.88 |
V'O2/kg [mL/(min.kg)] | 5.93 ± 1.28 | 6.27 ± 0.80 | 5.87 ± 1.30 | 6.20 ± 0.56 |
Anaerobic Threshold
| ||||
WR (Watt) | 58.6 ± 24.2 | 82.9 ± 29.1* | 54.5 ± 20.9 | 92.9 ± 31.1** |
Heart rate (beats/min) | 110 ± 14 | 111 ± 10 | 112 ± 13 | 118 ± 10# |
O2 pulse (mL/beat) | 7.69 ± 1.50 | 9.19 ± 2.42 | 7.01 ± 1.74 # | 9.48 ± 2.69** |
V'O2/kg [mL/(min.kg)] | 12.8 ± 3.0 | 16.7 ± 4.0** | 11.9 ± 2.9 | 18.0 ± 4.6**# |
V'E/V'CO2 | 29.2 ± 2.3 | 26.9 ± 1.5** | 29.1 ± 3.7 | 25.7 ± 2.0**# |
Maximal exercise
| ||||
WR (Watt) | 132 ± 30 | 188 ± 46** | 125 ± 35 | 196 ± 51** ## |
Heart rate (beats/min) | 158 ± 20 | 167 ± 8 | 155 ± 21 | 168 ± 8* |
O2 pulse (mL/beat) | 9.19 ± 2.18 | 12.43 ± 5.25* | 8.82 ± 2.20. | 11.70 ± 3.01** |
V'O2/kg [mL/(min.kg)] | 22.3 ± 5.7 | 31.2 ± 7.0** | 20.9 ± 5.5 ## | 31.9 ± 7.4**# |
CPET2 minus CPET1 | ||
---|---|---|
Patients n = 15 | Controls n = 15 | |
FVC (L) | 0.01 ± 0.21 | 0.05 ± 0.18 |
FEV 1 (L) | 0.00 ± 0.32 | 0.07 ± 0.17 |
Rest
| ||
Heart rate (beats/min) | 1.33 ± 6.29 | 0.20 ± 6.24 |
O2 pulse (mL/beat) | -0.17 ± 0.41 | 0.01 ± 0.47 |
V'O2/kg [mL/(min.kg)] | -0.07 ± 0.70 | -0.07 ± 0.80 |
Anaerobic Threshold
| ||
WR (Watt) | -4.40 ± 9.66 | 7.67 ± 19.50* |
Heart rate (beats/min) | 2.60 ± 7.79 | 4.60 ± 10.16 |
O2 pulse (ml/beat) | -0.67 ± 0.93 | 0.25 ± 1.09* |
V'O2/kg [mL/(min.kg)] | -0.87 ± 1.77 | 1.07 ± 2.63* |
V'E/V'CO2 | -0.21 ± 2.05 | -13.8 ± 50.1 |
Maximal exercise
| ||
WR (Watt) | -6.3 ± 11.5 | 11.1 ± 18.3** |
Heart rate (beats/min) | -3.3 ± 8.6 | 11.9 ± 41.6 |
O2 pulse (mL/beat) | -0.37 ± 0.70 | -4.6 ± 15.0 |
V'O2/kg [mL/(min.kg)] | -1.33 ± 1.68 | 0.73 ± 1.39** |
ATP synthesis in PBMC
CPET1 | CPET2 | |||
---|---|---|---|---|
Patients n = 15 | Controls n = 15 | Patients n = 15 | Controls n = 15 | |
Citrate synthase (CS) U/g protein | 135 ± 61 | 132 ± 17 | 128 ± 20 | 154 ± 51 |
ATP synthesis via Complex I nmol/(0.5 h. mg protein) | 7.1 ± 3.1 | 7.8 ± 2.8 | 6.7 ± 4.8 | 9.5 ± 5.7 |
ATP synthesis via Complex I nmol/(0.5 h. U CS) | 54 ± 19 | 58 ± 21 | 54 ± 34 | 61 ± 26 |
ATP synthesis via Complex II nmol/(0.5 h. mg protein) | 7.8 ± 5.0 | 8.2 ± 2.8 | 6.8 ± 4.9 | 8.9 ± 5.3 |
ATP synthesis via Complex II nmol/(0.5 h. U CS) | 58 ± 22 | 60 ± 26 | 54 ± 36 | 58 ± 27 |
CK in plasma U/L | 70 ± 25 | 83 ± 35 | 64 ± 22 | 96 ± 63 |