Introduction
Materials and methods
Subjects
Surgical implantation of intracerebroventricular (ICV) cannula
Treatment groups and intracerebroventricular infusion procedure
Apparatus: automated activity monitors
Behavioral testing
Lipid extraction
Electrospray ionization mass spectrometry analysis (ESI-MS)
Statistical analysis
Results
Behavioral assessment
Lipid analysis
Molecular weight | Molecular species | Base/acyl species | PBS | PPA |
---|---|---|---|---|
703 | 34:1 | 18:1/16:0 | 0.07 ± 0.03 | 0.10 ± 0.05 |
733 | 36:0 | 18:0/18:0 | 21.96 ± 0.35 | 39.13 ± 1.35* |
731 | 36:1 | 18:1/18:0 | 6.21 ± 0.67 | 4.53 ± 1.28 |
761 | 38:0 | 18:0/20:0 | 38.09 ± 0.84 | 29.37 ±1.92* |
759 | 38:1 | 18:1/20:0 | 16.08 ± 0.19 | 12.31 ± 3.64* |
789 | 40:0 | 18:0/22:0 | 13.03 ± 0.24 | 9.92 ± 1.31* |
811 | 42:3 | 18:1/24:2 | 4.56 ± 0.12 | 4.64 ± 1.54 |
Total%
|
100
|
100
| ||
∑ Saturates
|
73.07 ± 0.15
|
78.41 ± 1.44*
| ||
∑ Monounsat
|
22.37 ± 0.66
|
16.94 ± 2.91*
| ||
∑ Polyunsat
|
4.55 ± 0.12
|
4.63 ± 1.54
|
Molecular weight | Molecular species | Base/acyl species | PBS | PPA |
---|---|---|---|---|
703 | 34:1 | 18:1/16:0 | 14.05 ± 1.42 | 4.63 ± 1.10* |
733 | 36:0 | 18:0/18:0 | 2.63 ± 0.47 | 3.40 ± 1.23 |
731 | 36:1 | 18:1/18:0 | 1.54 ± 0.32 | 1.65 ± 0.42 |
761 | 38:0 | 18:0/20:0 | 12.91 ± 0.37 | 15.92 ± 0.65* |
759 | 38:1 | 18:1/20:0 | 8.36 ± 0.56 | 5.77 ± 0.24* |
789 | 40:0 | 18:0/22:0 | 9.44 ± 2.89 | 10.39 ± 2.03 |
811 | 42:3 | 18:1/24:2 | 51.07 ± 0.62 | 58.24 ± 0.68* |
Total%
|
100
|
100
| ||
∑ Saturates
|
24.98 ± 1.33
|
29.72 ± 1.49*
| ||
∑ Monounsat
|
23.95 ± 1.53
|
12.05 ± 1.57*
| ||
∑ Polyunsat
|
51.06 ± 0.63
|
58.22 ± 0.68*
|
Molecular weight | Molecular species | Plasmalogen or diacyl species | PBS | PPA |
---|---|---|---|---|
706 | D30:0 | 16:0/14:0 | 0.13 ± 0.055 | 0.13 ± 0.04 |
734 | D32:0 | 16:0/16:0 | 19.23 ± 0.34 | 19.33 ± 0.69 |
718 | P32:1 | p16:0/16:1 | 0.05 ± 0.01 | 0.06 ± 0.01 |
762 | D34:0 | 16:0/18:0 | 18.66 ± 0.49 | 19.14 ± 0.63 |
760 | D34:1 | 16:0/18:1 | 32.80 ± 0.15 | 33.68 ± 0.16* |
746 | P34:1 | p16:0/18:1 | 1.54 ± 0.26 | 1.40 ± 0.32 |
758 | D34:2 | 16:0/18:2 | 0.27 ± 0.04 | 0.69 ± 0.19* |
788 | D36:1 | 18:0/18:1 | 10.53 ± 0.18 | 9.20 ± 0.15* |
774 | P36:1 | p18:0/18:1 | 0.50 ± 0.11 | 0.40 ± 0.11 |
782 | D36:4 | 16:0/20:4 | 3.96 ± 0.51 | 4.81 ± 0.53* |
814 | D38:2 | 18:1/20:1 | 1.21 ± 0.25 | 1.29 ± 0.11 |
812 | D38:3 | 18:0/20:3 | 1.85 ± 0.28 | 1.74 ± 0.14 |
810 | D38:4 | 18:0/20:4 | 4.91 ± 0.26 | 4.33 ± 0.34 |
806 | D38:6 | 16:0/22:6 | 2.98 ± 0.12 | 2.74 ± 0.10 |
834 | D40:6 | 18:0/22:6 | 1.36 ± 0.11 | 1.06 ± 0.14 |
Total%
|
100
|
100
| ||
∑ Saturates
|
18.78 ± 0.48
|
19.27 ± 0.60
| ||
∑ Monounsat
|
62.56 ± 0.51
|
62.20 ± 0.70
| ||
∑ Polyunsat
|
16.55 ± 0.63
|
16.65 ± 0.99
| ||
∑ Plas
|
2.09 ± 0.32
|
1.85 ± 0.43
|
Molecular weight | Molecular species | Plasmalogen or diacyl species | PBS | PPA |
---|---|---|---|---|
706 | D30:0 | 16:0/14:0 | 0.79 ± 0.42 | 0.59 ± 0.18 |
734 | D32:0 | 16:0/16:0 | 1.39 ± 0.57 | 2.08 ± 0.14 |
718 | P32:1 | p16:0/16:1 | 0.52 ± 0.39 | 0.40 ± 0.053 |
762 | D34:0 | 16:0/18:0 | 2.90 ± 0.34 | 3.80 ± 0.32* |
760 | D34:1 | 16:0/18:1 | 9.03 ± 0.73 | 9.98 ± 1.81 |
746 | P34:1 | p16:0/18:1 | 0.61 ± 0.28 | 0.52 ± 0.05 |
758 | D34:2 | 16:0/18:2 | 20.13 ± 0.23 | 17.61 ± 0.16* |
788 | D36:1 | 18:0/18:1 | 8.11 ± 0.40 | 9.87 ± 0.38* |
774 | P36:1 | p18:0/18:1 | 0.76 ± 0.24 | 6.24 ± 1.96* |
782 | D36:4 | 16:0/20:4 | 13.23 ± 0.42 | 11.41 ± 0.22* |
814 | D38:2 | 18:1/20:1 | 4.14 ± 0.24 | 2.97 ± 0.45* |
812 | D38:3 | 18:0/20:3 | 9.82 ± 2.05 | 8.70 ± 0.64 |
810 | D38:4 | 18:0/20:4 | 23.92 ± 0.41 | 21.45 ± 0.87* |
806 | D38:6 | 16:0/22:6 | 2.42 ± 0.64 | 2.60 ± 0.63 |
834 | D40:6 | 18:0/22:6 | 2.23 ± 0.68 | 1.78 ± 0.58 |
Total%
|
100
|
100
| ||
∑ Saturates
|
3.68 ± 0.58
|
4.39 ± 1.21
| ||
∑ Monounsat
|
18.52 ± 1.57
|
21.92 ± 2.25
| ||
∑ Polyunsat
|
75.89 ± 2.55
|
66.52 ± 2.09*
| ||
∑ Plas
|
1.89 ± 0.86
|
7.15 ± 1.84*
|
Molecular weight | Molecular species | Diacyl species | PBS | PPA |
---|---|---|---|---|
835 | D34:1 | 16:0/18:1 | 0.53 ± 0.05 | 0.54 ± 0.07 |
859 | D36:3 | 18:1/18:2 | 1.01 ± 0.14 | 0.79 ± 0.01* |
857 | D36:4 | 16:0/20:4 | 7.71 ± 0.32 | 7.02 ± 0.45 |
887 | D38:3 | 18:0/20:3 | 9.07 ± 0.69 | 9.71 ± 0.73 |
885 | D38:4 | 18:0/20:4 | 74.17 ± 1.35 | 74.60 ± 1.27 |
883 | D38:5 | 18:1/20:4 | 5.53 ± 0.73 | 5.44 ± 0.41 |
881 | D38:6 | 16:0/22:6 | 0.80 ± 0.04 | 0.79 ± 0.04 |
913 | D40:4 | 18:0/22:4 | 0.41 ± 0.01 | 0.17 ± 0.03* |
909 | D40:6 | 18:0/22:6 | 0.77 ± 0.10 | 0.94 ± 0.12 |
Total%
|
100
|
100
| ||
∑ Monounsat
|
0.53 ± 0.05
|
0.54 ± 0.07
| ||
∑ Polyunsat
|
99.46 ± 0.05
|
99.46 ± 0.07
|
Molecular weight | Molecular species | Diacyl/species | PBS | PPA |
---|---|---|---|---|
835 | D34:1 | 16:0/18:1 | 6.71 ± 0.66 | 9.04 ± 0.39* |
859 | D36:3 | 18:1/18:2 | 6.16 ± 0.29 | 8.67 ± 0.97* |
857 | D36:4 | 16:0/20:4 | 8.33 ± 1.03 | 8.70 ± 0.85 |
887 | D38:3 | 18:0/20:3 | 8.53 ± 0.63 | 7.72 ± 0.69 |
885 | D38:4 | 18:0/20:4 | 44.00 ± 2.19 | 32.68 ± 3.6* |
883 | D38:5 | 18:1/20:4 | 6.67 ± 0.56 | 7.79 ± 0.28 |
881 | D38:6 | 16:0/22:6 | 6.17 ± 0.04 | 7.89 ± 0.50* |
913 | D40:4 | 18:0/22:4 | 6.50 ± 0.60 | 8.47 ± 0.31* |
909 | D40:6 | 18:0/22:6 | 6.93 ± 0.54 | 9.04 ± 0.38* |
Total%
|
100
|
100
| ||
∑ Monounsat
|
6.70 ± 0.66
|
9.03 ± 1.03
| ||
∑ Polyunsat
|
93.29 ± 0.66
|
90.96 ± 1.03
|
Molecular weight | Molecular species | Diacyl species | PBS | PPA |
---|---|---|---|---|
760 | D34:1 | 16:0/18:1 | 0.90 ± 0.10 | 1.10 ± 0.21 |
790 | D36:0 | 18:0/18:0 | 1.59 ± 0.41 | 1.65 ± 0.11 |
788 | D36:1 | 18:0/18:1 | 18.10 ± 0.53 | 21.62 ± 0.63* |
786 | D36:2 | 18:0/18:2 | 2.36 ± 0.34 | 2.55 ± 0.17 |
816 | D38:1 | 18:0/20:1 | 1.00 ± 0.15 | 1.22 ± 0.19 |
810 | D38:4 | 18:0/20:4 | 4.18 ± 0.07 | 4.46 ± 0.12 |
840 | D40:3 | 18:0/22:3 | 1.19 ± 0.23 | 1.20 ± 0.17 |
838 | D40:4 | 18:0/22:4 | 4.67 ± 0.17 | 3.63 ± 0.14* |
836 | D40:5 | 18:0/22:5 | 6.40 ± 0.30 | 6.91 ± 0.43 |
834 | D40:6 | 18:0/22:6 | 58.39 ± 0.98 | 54.49 ± 1.05* |
832 | D40:7 | 18:1/22:6 | 1.22 ± 0.08 | 1.17 ± 0.12 |
Total%
|
100
|
100
| ||
∑ Saturates
|
1.59 ± 0.15
|
1.65 ± 0.11
| ||
∑ Monounsat
|
19.99 ± 0.39
|
23.93 ± 0.66*
| ||
∑ Polyunsat
|
78.41 ± 0.58
|
74.45 ± 0.55*
|
Molecular weight | Molecular species | Diacyl species | PBS | PPA |
---|---|---|---|---|
762 | D34:1 | 16:0/18:1 | 6.55 ± 0.30 | 7.94 ± 0.09* |
792 | D36:0 | 18:0/18:0 | 8.12 ± 0.57 | 7.62 ± 0.24 |
790 | D36:1 | 18:0/18:1 | 9.53 ± 0.74 | 12.13 ± 1.21* |
788 | D36:2 | 18:0/18:2 | 7.81 ± 0.06 | 8.84 ± 0.63* |
818 | D38:1 | 18:0/20:1 | 7.72 ± 0.24 | 6.94 ± 0.63 |
812 | D38:4 | 18:0/20:4 | 11.55 ± 1.36 | 11.76 ± 1.65 |
842 | D40:3 | 18:0/22:3 | 8.64 ± 0.53 | 7.37 ± 0.51* |
840 | D40:4 | 18:0/22:4 | 7.81 ± 0.08 | 7.50 ± 0.35 |
838 | D40:5 | 18:0/22:5 | 7.61 ± 0.23 | 7.69 ± 0.06 |
836 | D40:6 | 18:0/22:6 | 16.67 ± 1.34 | 13.68 ± 0.88* |
834 | D40:7 | 18:1/22:6 | 7.99 ± 0.37 | 8.53 ± 0.19 |
Total%
|
100
|
100
| ||
∑ Saturates
|
8.12 ± 0.57
|
7.62 ± 0.24
| ||
∑ Monounsat
|
23.80 ± 1.76
|
27.02 ± 1.99*
| ||
∑ Polyunsat
|
68.06 ± 0.99
|
65.34 ± 1.18*
|
Molecular weight | Molecular species | Plasmalogen or diacyl species | PBS | PPA |
---|---|---|---|---|
700 | P34:1 | p16:0/18:1 | 5.85 ± 0.15 | 5.10 ± 0.14* |
714 | D34:2 | 16:0/18:2 | 3.37 ± 0.17 | 3.49 ± 0.15 |
730 | P36:0 | p18:0/18:0 | 3.42 ± 0.13 | 4.03 ± 0.38 |
744 | D36:1 | 18:0/18:1 | 3.50 ± 0.46 | 3.32 ± 0.37 |
728 | P36:1 | p18:0/18:1 | 6.00 ± 0.09 | 6.82 ± 0.12* |
742 | D36:2 | 18:0/18:2 | 3.68 ± 0.14 | 3.59 ± 0.35 |
726 | P36:2 | p18:0/18:2 | 4.79 ± 0.30 | 7.16 ± 0.62* |
724 | P36:3 | p18:1/18:2 | 3.38 ± 0.42 | 2.98 ± 0.27 |
722 | P36:4 | p16:0/20:4 | 4.32 ± 0.12 | 4.43 ± 0.14 |
766 | D38:4 | 18:0/20:4 | 6.99 ± 0.71 | 7.33 ± 0.62 |
750 | P38:4 | p18:0/20:4 | 6.07 ± 0.13 | 7.70 ± 0.02* |
762 | D38:6 | 16:0/22:6 | 4.18 ± 0.16 | 3.45 ± 0.15* |
746 | P38:6 | p16:0/22:6 | 5.14 ± 0.45 | 5.11 ± 0.80 |
796 | D40:3 | 18:0/22:3 | 3.59 ± 0.26 | 3.52 ± 0.43 |
794 | D40:4 | 20:0/20:4 | 3.51 ± 0.08 | 3.85 ± 0.42 |
778 | P40:4 | p18:0/22:4 | 3.46 ± 0.26 | 5.00 ± 0.10* |
792 | D40:5 | 18:0/22:5 | 4.30 ± 0.31 | 3.93 ± 0.41 |
776 | P40:5 | p18:0/22:5 | 3.91 ± 0.69 | 3.79 ± 0.60 |
790 | D40:6 | 18:0/22:6 | 11.07 ± 1.06 | 8.59 ± 0.36* |
774 | P40:6 | p18:0/22:6 | 9.47 ± 0.21 | 6.82 ± 0.86* |
Total%
|
100
|
100
| ||
∑ Saturates
|
3.42 ± 0.12
|
4.02 ± 0.38
| ||
∑ Monounsat
|
15.32 ± 0.65
|
15.23 ± 0.92
| ||
∑ Polyunsat
|
81.23 ± 0.69
|
80.73 ± 0.55
| ||
∑ Plas
|
55.82 ± 1.63
|
58.93 ± 1.40
|
Molecular weight | Molecular species | Plasmalogen or diacyl species | PBS | PPA |
---|---|---|---|---|
702 | P34:1 | p16:0/18:1 | 4.59 ± 0.43 | 3.51 ± 0.53 |
716 | D34:2 | 16:0/18:2 | 4.42 ± 0.13 | 4.41 ± 0.66 |
732 | P36:0 | p18:0/18:0 | 4.39 ± 0.19 | 3.11 ± 0.41* |
746 | D36:1 | 18:0/18:1 | 4.55 ± 0.44 | 3.38 ± 0.49* |
730 | P36:1 | p18:0/18:1 | 5.76 ± 0.56 | 3.57 ± 0.50 * |
744 | D36:2 | 18:0/18:2 | 5.20 ± 0.58 | 4.49 ± 0.55 |
728 | P36:2 | p18:0/18:2 | 3.10 ± 1.36 | 3.19 ± 0.26 |
726 | P36:3 | p18:1/18:2 | 4.28 ± 0.19 | 3.85 ± 0.36 |
724 | P36:4 | p16:0/20:4 | 4.47 ± 0.42 | 3.41 ± 0.46* |
768 | D38:4 | 18:0/20:4 | 10.82 ± 0.71 | 23.60 ± 4.93* |
752 | P38:4 | p18:0/20:4 | 4.67 ± 0.06 | 3.70 ± 0.45* |
764 | D38:6 | 16:0/22:6 | 5.35 ± 0.21 | 7.02 ± 0.56* |
748 | P38:6 | p16:0/22:6 | 4.87 ± 0.38 | 3.71 ± 0.64 |
798 | D40:3 | 18:0/22:3 | 4.27 ± 0.34 | 3.92 ± 0.68 |
796 | D40:4 | 20:0/20:4 | 4.78 ± 0.29 | 3.67 ± 0.62 |
780 | P40:4 | p18:0/22:4 | 4.06 ± 0.17 | 3.41 ± 0.34 |
794 | D40:5 | 18:0/22:5 | 4.97 ± 0.35 | 4.07 ± 0.32 |
778 | P40:5 | p18:0/22:5 | 4.67 ± 0.27 | 3.76 ± 0.83 |
792 | D40:6 | 18:0/22:6 | 5.89 ± 0.62 | 7.03 ± 1.63 |
776 | P40:6 | p18:0/22:6 | 4.80 ± 0.33 | 3.54 ± 0.69* |
Total%
|
100
|
100
| ||
∑ Saturates
|
4.39 ± 0.19
|
3.11 ± 0.41*
| ||
∑ Monounsat
|
14.92 ± 0.39
|
10.46 ± 1.46*
| ||
∑ Polyunsat
|
80.68 ± 0.43
|
86.41 ± 1.81*
| ||
∑ Plas
|
49.69 ± 0.96
|
38.37 ± 3.81*
|