Introduction
Material and methods
Study subjects
Real-time quantitative PCR for HBV DNA
Serology
Data management
Results
Performance of the real-time qPCR
DNA (copies DNA per mL) in experiment | ||||||
---|---|---|---|---|---|---|
Sample ID | Experiment 1 | Experiment 2 | Experiment 3 | Average | SD | CV (%) |
MVA 300 | 2.6 × 106 | 2.5 × 106 | 1.6 × 106 | 2.3 × 106 | 0.104 | 1.60 |
MVA 307 | 1.9 × 109 | 4.5 × 109 | 1.7 × 109 | 2.7 × 109 | 0.191 | 2.00 |
MVA 313 | 1.2 × 108 | 1.2 × 108 | 1.2 × 108 | 1.2 × 108 | 0.004 | 0.50 |
MVA 315 | 2.5 × 106 | 2.2 × 106 | 1.9 × 106 | 2.2 × 106 | 0.046 | 0.70 |
T4040 | 4.7 × 103 | 4.9 × 103 | 6.4 × 103 | 5.4 × 103 | 0.060 | 1.60 |
Negative control | not detected | not detected | not detected |
DNA (copies per mL) on different days | ||||||
---|---|---|---|---|---|---|
Sample ID | Day 1 | Day 2 | Day 3 | Average | SD | CV (%) |
MVA300 | 2.7 × 106 | 1.4 × 106 | 2.7 × 106 | 2.3 × 106 | 0.19 | 3.0 |
MVA307 | 2.1 × 109 | 4.2 × 109 | 1.5 × 109 | 2.6 × 106 | 0.26 | 2.70 |
MVA313 | 1.4 x108 | 9.8 × 107 | 1.3 × 108 | 1.2 × 108 | 0.09 | 1.10 |
MVA315 | 1.3 × 106 | 3.3 × 106 | 2.0 x106 | 2.2 × 106 | 0.01 | 1.20 |
T4040 | 5.6 × 103 | 7.2 × 103 | 4.3 × 103 | 5.7 × 103 | 0.23 | 0.62 |
Negative control | DNA not detected | DNA not detected | DNA not detected | DNA not detected |
Correlation of HBV DNA loads and HBeAg in asymptomatic carriers
HBeAg Positive subjects |
HBeAg Negative carriers
| ||||
---|---|---|---|---|---|
Age group (Yrs) | DNA positive (%) | Viral load (GM) | DNA positive (%) | Viral load (GM) | Total DNA positive in all carriers |
<5 | 50/50 (100%) | 3.2 × 108 | 9/9 (100%) | 4.3 × 104 | 59/69 (85.5%) |
5–9 | 39/39 (100%) | 3.8 × 108 | 15/21 (71.4%) | 9.3 × 104 | 54/63(85.7%) |
10–19 | 5/5 (100%) | 1.7 × 109 | 11/13 (84.6%) | 1.3 × 104 | 16/18 (88.9%) |
20–29 | 11/11 (100%) | 8.1 X107 | 71/91 (78.0%) | 4.9 X103 | 82/102 (80%) |
30–39 | 3/3 (100%) | 5.5 X107 | 23/33 (69.6%) | 2.6 X103 | 26/36 (72%) |
>40 | 1/1 (100%) | 4.6 X102 | 32/42 (76.1%) | 6.02 X103 | 33/43 (77%) |
Total | 109/109 (100%) | 161/209 (77.0%) | 270 |