Background
Spinal cord ischemia reperfusion (IR) injuries have garnered much attention since 1986, when an IR injury was first reported, as they are associated with severe complications such as bladder, bowel, sexual dysfunction, and paraplegia [
1]. Spinal cord IR injuries can induce a cascade of secondary events, such as neuronal or glial insults, that lead to further cell loss and behavioral impairments. These, in turn, are closely associated with inflammatory responses, including the release of cytokines, chemokines, and the recruitment of immune cells [
2-
4]. Numerous studies have shown that the major effectors involved in this inflammatory cascade activate the Toll-like receptors (TLRs), and the neuroprotective effect of inhibiting the TLR
4 inflammatory pathway has been extensively studied by us and others [
5-
8].
We previously demonstrated that intrathecal antagonism of TLR
4 in a rat model of IR, induced by transient occlusion of the aortic arch, could maintain the integrity of the blood-spinal cord barrier (BSCB) and attenuate secondary spinal cord injury by inhibiting proinflammatory cytokines, molecules downstream of the TLR
4 pathway [
6,
7]. Contrary to previous investigations that showed that Myeloid differentiation factor
88
(MyD
88) and MyD88 adaptor-like (Mal/TIRAP) adaptors functioned downstream of TLR
4, the adapter TIR-containing adaptor molecule-2 (TICAM-2, also known as TRAM) was shown to resemble Mal/TIRAP and to physically bridge it with TICAM-1 to functionally transmit the TLR
4 signal [
9,
10]. Therefore, TLR
4 recruits two crucial adaptors, TIRAP and TICAM-2, which are connected to two effective adapters, MyD
88 and TICAM-1, respectively, after activation [
9].
MicroRNAs (miRs) are small, non-coding RNAs that are capable of specific binding to one or more target mRNAs, and effectively regulate their post-transcriptional expression in various tissues [
11-
13]. Studies have shown that several miRs can dramatically alter normal physiological processes and are involved in the pathogenesis of various diseases [
14,
15]. Recent studies showed that ischemia alters the expression of miRs in cardiac tissue, and antagomirs against miRs improved neovascularization and augmented functional recovery in a large animal model of cardiac IR injury, suggesting a role for miRs in the regulation of IR [
16,
17]. Furthermore, there is also increasing evidence for the involvement of miRs in traumatic spinal cord injury [
18,
19]. However, the role of miRs in IR is not well understood. Exploring the expression profiles of miRs altered after IR might reveal whether miR-dependent post-transcriptional gene regulation in TLR
4-mediated inflammation determines the progression of, and recovery from, secondary damage to the spinal cord.
In this study, we first used miR arrays to determine the expression patterns of miRs in a rat model of spinal cord IR injury, and then identified their target mRNAs by searching the TargetScan, MicroCosm Targets (version 5), and microRNA.org databases. Among the miRs identified, we observed that miR-27a was one of the most dysregulated miRs, and further defined TICAM-2, a key regulator of the TLR4 pathway, as its target. Then, the effects of miR-27a were assessed in a rat model of IR by intrathecal pretreatment with an miR mimic and an anti-miR oligonucleotide (AMO) starting three days before ischemia. Our results demonstrated that increasing the expression of miR-27a attenuated IR-induced spinal cord injury by negatively modulating the TLR4 signaling regulator TICAM-2, which may be a new therapeutic target under neuroinflammatory conditions.
Materials and methods
Experimental animals
The experimental procedures were performed in accordance with the Guide for the Care and Use of Laboratory Animals (United States National Institutes of Health publication number 85–23, National Academy Press, Washington DC, revised 1996) The animals used in this study were male Sprague-Dawley rats (obtained from Animal center of China Medical University, Shenyang, China) weighing between 200 and 250 g. The rats were bred in standard cages with free access to food and water, and were housed separately after surgery at First Affiliated Hospital of China Medical University.
Rat model of spinal cord ischemia reperfusion injury
To establish the spinal cord IR model, IR was induced by occluding the aortic arch for 14 minutes, as previously reported [
6,
7]. In brief, rats were anesthetized with an intraperitoneal injection of 4% sodium pentobarbital (Beyotime Biotechnology, Shanghai, China) at a dose of 50 mg/kg, and then the aortic arch was exposed through a cervicothoracic approach. Under direct visualization, the aortic arch was cross-clamped between the left common carotid artery and left subclavian artery. Occlusion was confirmed with a laser Doppler blood flow monitor (Moor Instruments, Axminster, Devon, United Kingdom). Ischemia, which is defined as a 90% decrease in the flow measured at the femoral artery{AU Query: If this definition originates from any official published guidelines, please provide a reference}, continued for 14 minutes, after which the clamps were removed and reperfusion was allowed to continue for either 24 or 72 hours. Sham-operated rats underwent the same procedure without aortic arch occlusion.
MiR microarray analysis
To assess MiR expression in the spine, L4–6 segments of the spinal cord were harvested at 24 and 72 hours after reperfusion, frozen in liquid nitrogen, and stored at −80°C until use. Total RNA was isolated from the samples using TRIzol® reagent (Invitrogen, Carlsbad, California, United States) and the miRNeasy mini kit (Qiagen, West Sussex, United Kingdom) according to the manufacturers’ instructions. After measuring the quantity of RNA using a NanoDrop 1000 (Youpu Scientific Instrument Co., Ltd., Shanghai, China), the samples were labeled using the miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) and hybridized on a miRCURY™ LNA Array (version 18.0, Exiqon, Vedbaek, Denmark). After washing, the slides were scanned using an Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, California, United States). Scanned images were then imported into the GenePix Pro 6.0 program (Axon Instruments) for grid alignment and data extraction. Replicated miRs were averaged, and miRs with intensities of 50 or more in all samples were used to calculate a normalization factor. Expressed data were normalized by median normalization. After normalization, the miRs that were significantly differentially expressed were identified by Volcano Plot filtering. Finally, hierarchical clustering was performed to determine the differences in the miR expression profiles among the samples by using MEV software (version 4.6; TIGR, Microarray Software Suite 4, Boston, United States).
Measurement of Evans blue extravasation
After 24 and 72 hours of reperfusion, Evans blue dye ((EB) 30 g/L; Sigma-Aldrich, Louis, United States) was intravenously injected (45 mg/kg) into the tail vein 60 minutes before the animals were euthanized. After adequate perfusion with saline under deep anesthesia, the L4–6 segments were removed, soaked in methanamide (Beyotime Biotechnology, Shanghai, China) for 24 hours at 60°C, and then centrifuged. EB content was measured as the absorbance of the supernatant at 632 nm on a microplate reader (BioTek, Winooski, Vermont, United States) and is reported as the amount of EB per wet tissue weight (μg/g). To measure the fluorescence, the tissue was fixed in 4% paraformaldehyde (Beyotime Biotechnology, Shanghai, China), sectioned (10 μm), sealed in a light-tight container, and frozen. EB staining was visualized using a BX-60 fluorescence microscope (Olympus, Melville, New York, United States) with a green filter.
Quantification of miR expression
MiR expression was quantified by using an Applied Biosystems 7500 Real-Time PCR System (Foster City, California, United States) to verify regulation of the miR targets in the spinal segments of the IR and sham groups. Total RNA from the L4–6 segments of the spinal cords was extracted with TRIzol reagent and reverse transcribed to cDNA with the PrimeScript® miRNA cDNA synthesis kit (Perfect Real Time; TaKaRa, Dalian, China) according to the manufacturers’ instructions. PCR was then used to amplify miR-27a using SYBR Premix Ex TaqTM II (Perfect Real Time; TaKaRa, Tokyo, Japan) and miR-27a-specific primers (forward, 5′-ACACTCCAGCTGGGTTCACAGTGGCTAAG-3′ and reverse, 5′-TGGTGTCGTGGAGTCG-3′; RiboBio, Guangzhou, China) at 95°C for 10 seconds, followed by 40 cycles of 95°C for five seconds and 60°C for 20 seconds. The primers used to amplify U6 were 5′-CTCGCTTCGGCAGCACA-3′ (forward) and 5′-AACGCTTCACGAATTTGCGT-3′ (reverse). All reactions were performed in triplicate. The relative expression of miR-27a was normalized to U6. Data were analyzed by using the 2−ΔΔCt method.
Intrathecal pretreatment with a synthetic miR mimic and an anti-miR oligonucleotide
The method used to pretreat rats with a mimic and an AMO of
miRNA-27a (GenBank number: [NR_031833.1]) and negative controls has been previously described [
17]. For intrathecal infusion, a laminectomy was performed at the level of the thoracic vertebrae under pentobarbital anesthesia (Beyotime Biotechnology, Shanghai, China). A polyethylene catheter (PE10, Portex, Kent, United Kingdom, inside diameter (ID): 0.28 mm and outside diameter (OD): 0.61 mm) was passed caudally from T
9–12, and 2 cm of the free end was left exposed in the upper thoracic region. We intrathecally infused 100 μL of a synthetic
miR-27a mimic (
mimic-27a), an AMO (
AMO-27a), or the negative control (
NC-27a, all at 50 mg/kg; Jima Inc., Shanghai, China) pretreated with Lipofectamine® 2000 (Invitrogen) continuously for three days before the surgical operation. The sequences of
mimic-27a,
AMO-27a, and
NC-27a were 5′-UUCACAGUGGCUAAGUUCCGC-3′, 5′-GCGGAACTTAGCCACTGTGAA-3′, and 5′-AAGGCAAGCUGACCCUGAAGUU-3′, respectively. To analyze the specificity and efficacy of the
miR-27a and
AMO-27a, real-time PCR was performed as described above.
Quantification of TLR4 and TICAM-2 mRNA
Quantitative real-time PCR was used to detect TLR
4 and TICAM-2 mRNA as previously described [
7]. Total RNA was extracted from L
4–6 spinal cord tissue using TRIzol reagent according to the manufacturer’s instructions. PCR was performed as described previously using SYBR Green SuperMix-UDG on a Prism 7000 Sequence Detection System (Applied Biosystems) and the following primers: TLR
4 (NM_0191178) forward, 5′-GGATGATGCCTCTCTTGCAT-3′ and reverse, 5′-TGATCCATGCATTGGTAGGTAA-3′; TICAM-2 (NM_021649) forward, 5′-GGGAATTCATAATGGGTATCGGGAAGTC-3′ and reverse, 5′-GGCTGCAGGTTATATGTTTCATCTCAGGC-3′; and GAPDH (glyceraldehyde-3-phosphate dehydrogenase, NM_023964) forward, 5′-AGAAGGCTGGGGCTCATTTG-3′ and reverse, 5′-AGGGGCCATCCACAGTCTTC-3′. Amplification was performed using the following cycling conditions: 50°C for two minutes (uracil-DNA glycosylase incubation), 95°C for 10 minutes, and 40 cycles of denaturation at 95°C for 15 seconds and annealing at 60°C for 30 seconds. All reactions were performed in triplicate. Gene expression was normalized to GAPDH (as an internal control). Data were analyzed by using the 2
-ΔΔCt method.
Double immunofluorescence staining for TLR4 and TICAM-2
Double immunofluorescence staining was performed as described previously to explore the interaction between TLR
4 and TICAM-2 after IR [
6,
7]. Briefly, 10-μm-thick sections were incubated with a primary rabbit anti-TLR
4 antibody (1:800; Abcam, Cambridge, United States) and a goat anti-TICAM-2 antibody (1:100; Santa Cruz Biotechnology, Santa Cruz, California, United States) overnight at 4°C. After incubation with an Alexa 488-conjugated donkey anti-rabbit immunoglobulin G (IgG) antibody (1:500; Molecular Probes, Eugene, United States) and an Alexa 594-conjugated donkey anti-goat IgG antibody (1:500; Molecular Probes), each for two hours at room temperature, images were captured using a Leica TCS SP2 laser scanning spectral confocal microscope (Leica Microsystems, Buffalo Grove, Illinois, United States).
Western blot analysis
The expression of TLR4, TICAM-2, and NF-κB p65 in spinal cord tissue was determined by western blot analyses. The rats’ spinal cords were homogenized, and total proteins were purified using tissue and nuclear protein extraction reagents according to the manufacturer’s instructions (KC-415 and KGP-150; KangChen, Shanghai, China). The antibodies against TLR4 (1:500; Abcam), TICAM-2 (1:500; Santa Cruz Biotechnology), and NF-κB p65 (1:500; Abcam) were used in this experiment, along with horseradish peroxidase-conjugated secondary antibodies (Bioss, Beijing, China). The scanned images were semi-quantitated using Quantity One software (Bio-Rad Laboratories, Milan, Italy).
Measurement of lnterleukin -1β content using ELISA
The spinal cord was collected, homogenized, and then centrifuged. lnterleukin (IL)-1β content was determined with an ELISA kit (R&D Systems, Minneapolis, Minnesota, United States) according to the manufacturer’s instructions. Absorbance (A) was measured at 450 nm, and the IL-1β content of each sample was calculated based on a standard curve and was expressed in pictograms per milligram of total protein.
Statistical analysis
All data were expressed as mean ± standard error of the mean (means ± SEM) and were analyzed with SPSS software (version 17.0; SPSS Inc., Chicago, Illinois, United States). All variables measured in this study were normally distributed, and the groups were compared with Student’s t-test or one-way analysis of variance (ANOVA), followed by Newman-Keuls post-hoc analysis. A P value less than 0.05 was considered to be statistically significant.
Discussion
The BSCB is a physical and biochemical barrier between the circulation and the spinal cord that plays an important role in the regulation of spinal cord homeostasis. Disruption of BSCB integrity after IR injury has been reported to trigger alterations in the spinal microenvironment, allowing penetration of both inflammatory cytokines and immune cells into the spinal cord, which determines the prognosis of patients with IR injury [
6,
7,
22]. Accumulating evidence indicates that some of the major effectors in this inflammatory damage to BSCB are engagement with toll-like receptors (the TLRs) signal pathway [
5-
8,
23]. Recently, many studies have shown that individual miRs, endogenous, non-coding, single-stranded, small RNAs that are widespread in eukaryotic organisms, are capable of affecting numerous target mRNAs and effectively regulating their gene expression [
8,
15,
18,
19]. Altering the expression of miRs greatly affects the pathogenesis of IR and its functional outcome [
16,
24]. Therefore, in this present study, we provide evidences that miRs attenuate BSCB permeability, and our findings expand the understanding of the molecular mechanisms underlying TLR
4-mediated inflammatory responses. To the best of our knowledge, this is the first study to document a
miR-27a target related to the TLR
4 pathway in spinal cord IR injury.
MiRs are a class of sophisticated gene expression regulators with the unique ability to prevent the translation of and/or degrade their corresponding target mRNAs by inhibiting translation and promoting cleavage, respectively. MiRs act as key regulators in a wide variety of biological processes, including cell proliferation, differentiation, apoptosis, and organ development, and have also been implicated in inflammatory diseases [
25,
26]. Previous studies showed that some miRs may regulate genes associated with TLR signaling pathways; the mRNA expression of five adaptor and interacting proteins (CD14, HSPA1a, Pglyrp-1, MD-1, and TICAM-2) were significantly upregulated following traumatic brain injury (TBI) [
8,
23]. In this study, using a miRs microarray screening approach, we found that
miR-27a expression was significantly altered at both 24 h and 72 hours after IR, and such results were as confirmed by qRT-qPCR (Figure
2).
In animals, recognition of miR response elements only requires a continuous 6six-base pair ‘“seed match”’ near the 3′-UTR of its target mRNA [
27]. Given this, many target genes of
miR-27a have been identified, some of which were showed to participated in innate immune response and inflammatory responses [
28-
31]. Whether
miR-27a specifically target TICAM-2
in vivo remainsed to be tested. It hasis recently been shown that pretreatment with miR mimics and AMOs was is one of the most common and useful methods to regulate the expression levels of miRs [
16,
17,
28,
29]. He
et al. reported that injecting miR mimics 48 hours before IR greatly downregulated the levels of apoptosis-related genes, whereas injecting the corresponding AMOs upregulated their expression [
17]. Our
in vivo data, shown in Figure
4a, b, c, are in close accordance with previous studies, showing that compensating for decreased
miR-27a levels by continuous intrathecal injection of
mimic-27a into the subarachnoid space of IR model rats, reduced the mRNA and protein expression of TICAM-2 by inhibiting the translation and/or promoting the degradation of its mRNA. Continuous intrathecal injection of
AMO-27a before ischemia clearly abrogated reversed such changes. However, no obvious changes were detected when model animals were pretreated with
NC-27a, suggesting that
miR-27a directly modulates TICAM-2 expression i
n vivo, and that modulation of
miR-27a is particularly important in the pathophysiology of IR.
TICAM-2 is a cytoplasmic protein that structurally resembles the MAL/TIRAP adaptor that links TLR
4 and MyD
88 and functionally transmits TLR
4 signaling to TICAM-1 [
9,
32]. Given the bridging action between TLR
4 and TRIF, TICAM-2 coordinates the inflammatory response to a pathogen challenge [
33]. Thus, it is easy to postulate that IR-induced aberrant TICAM-2 expression might be closely associated with TLR
4 activation, subsequent NF-κB relocation to the nucleus, and the release of downstream proinflammatory cytokines [
9,
32,
34]. Consistent with this, here, we observe that TLR
4 expression is also significantly upregulated when TICAM-2 expression increased and
miR-27a decreased at both 24 and 72 hours after IR (Figure
4a, b, c, d, e). In addition, the double immunofluorescence staining in Figure
5 shows that the signal for TLR
4, a membrane-bound receptor, is greatly upregulated coincident along with increased cytoplasmic staining of TICAM-2 in neuronal and glial cells of ischemic spinal cords. Therefore, treatments that decrease TICAM-2 and inhibit the inflammatory stimulation of TLR
4 might be a novel intervention for IR [
9,
32,
33,
35]. We then identifiedy the mechanism underlying miR-dependent regulation of TICAM-2 expression. Intrathecal injection of
mimic-27a prevented the increase in TICAM-2 immunoreactivity and the number of double-labeled cells, whereas intrathecal injection of
AMO-27a reversed these effects. In accordance with these observed effects of mimic-27a and
AMO-27a on TICAM-2, we observed similar expression profiles for TLR
4, NF-κB, and the proinflammatory cytokine IL-1β at 24 and 72 hours post-injury, providing direct evidence that IR-induced TLR
4 activation is significantly influenced by the expression of TICAM-2.
Proinflammatory cytokines are important molecules in the immune system that have been implicated in alterations of BSCB integrity [
6,
7,
36,
37]. Breakdown of the BSCB allows exogenous pathogens and circulating immune cells to enter the spinal cord, which has numerous consequences, including neuronal loss, central sensitization, and glial remodeling [
22,
36]. Inhibiting inflammatory damage to the BSCB has been postulated as thea key to protection in IR. Our present study provides clear evidence for the protective effects of specifically increasing
miR-27a expression. An Iinjection of
mimic-27a attenuated BSCB dysfunction, which was manifested as reduced fluorescent dye and EB extravasation, and was suppressed by the injection of
AMO-27a; this finding indicatesd that
miR-27a regulates inflammatory damage to the BSCB by targeting the TICAM-2 mRNA and the TLR
4/NF-κB/IL-1β pathway.
Significantly, one major character of miRs is that a single miR is capable of regulating the expression of many target genes, whereas a target gene can also be regulated by several miRs [
11,
13,
26]. Thus, it is very possible to gain different or even contradictory expressions when exploring the same miR in differently experimental conditions [
20,
28,
38,
39]. For example, in the recsent study of Young
et al. showed the opposite role of
miR-27a in the regulation of vascular leaking by targeting vascular endothelium (VE)-cadherin in the endothelium [
38]. On the other hand, some studies showed an upregulation of
miR-27a for systemic inflammation, instead of the down-regulation as described in this study in the event of systemic inflammation [
28,
39]. These disagreements might be caused by the different observation time points, which are consistent with the descriptions of that no changes being observed in
miR-27a expression for at 6 hours, instead of downregulation for at 24 hours after lipopolysaccharide (LPS) treatments [
28]. Moreover, the net effects of miRs observed in
in vivo experiments were also influenced by the complicated internal environment [
20,
40]. Luxenhofer G
et al. emphasized the importance of miRs in regulating the molecular network specifying the generation of neuronal diversity in the developing chick spinal cord [
40]. Ziu M
et al. also showed significantly different expression levels of miRs in prolonged compression injury compared to those in short compression injury [
20]. Furthermore, different expressions of miRs were reported in different regions of the injured spinal cord, even under the same experimental conditions [
20]. Thus, aseptically inflammatory responses during spinal cord IR injury could not be exactly equivalent to directly proinflammatory stimuli with LPS
in vitro as well as the results obtained from the lung tissue. Further
in vitro and
in vivo studies still need to be conducted to identify the correlation between
miR-27a and the corresponding target genes in the mode of inflammatory or anti-inflammatory actions to better elucidate the mechanism and provide potential therapeutic targets for IR.
Taken together, we identify TICAM-2 as a novel target of miR-27a and show that downregulation of miR-27a promotes IR-induced inflammatory damage to the BSCB by facilitating activation of the TLR4/NF-κB/IL-1β signaling pathway.
Competing interests
The authors declare that they have no competing interests.
Authors’ contributions
X-QL, Z-LW, and BF participated in animal care and made the animal models. X-QL, Z-LW, and W-FT prepared and sectioned tissues and performed most of the immunohistochemistry assays. X-QL, BF, and W-FT performed the western blot assays and the statistical analysis. Z-LW, BF, and H-WL conducted the miRNA microarray analysis and luciferase assays. HM guided the model design and study design. H-WL gave important directions for data analysis and manuscript writing. All authors read and approved the final manuscript.