Background
Acute appendicitis is one of the most frequent abdominal surgical emergencies. It occurs with a rate of 8.6% for males and 6.7% for females [
1]. Approximately 8% of the population will undergo appendectomy for acute appendicitis in their lifetime [
2]. Complicated appendicitis represents from 14 to 55% of all appendicitis cases and is defined as an acute appendicitis with peritonitis, rupture, gangrene or intra-abdominal abscess, intra-peritoneal presence of faecalith, intra-operative mass or four-quadrant purulent material, local or generalized peritonitis [
3,
4]. Some studies have demonstrated that complicated appendicitis is associated with higher rates of major post-operative complications [
5‐
8]. In children, perforated appendicitis has an incidence from 15 to 20% [
7] and can be responsible for post-operative morbidity [
1], especially related to post-operative IAA. In particular, perforated appendicitis leads more frequently to IAA [
8‐
10], increasing the risk of surgical site infection, hospital stay and cost. The rate of IAA after appendectomy for perforated appendicitis is variable: some studies report between 5 and 10%, others 3–20% [
9,
10]. Perforated appendicitis was recognized as an independent risk factor for development of post-operative IAA [
7‐
9]. Different studies defined a risk of post-operative IAA of 1–4% in non-perforated appendicitis [
7‐
9], compared with 10–24% after appendectomy for perforated appendicitis [
9,
10]. Diabetes mellitus, young and old age [
6], obesity and peritoneal irrigation were associated risk factors [
6,
8].
Laparoscopic appendectomy has become the standard surgical procedure for acute appendicitis in both the paediatric and adult population [
6,
11‐
13]. Recognized benefits are a more rapid recovery, less pain, fewer wound infections, shorter hospitalization and earlier return to daily activities [
6,
11,
14,
15]. Although appendectomy is considered a safe procedure, different post-operative complications may occur, such as intra-abdominal abscess (IAA), wound infection, bleeding and bowel obstruction.
Most frequent and fearsome complication is abscess that occurs in 2.2% after laparoscopic appendectomy [
16], but in both laparoscopic and open techniques, intra-abdominal abscess seems to be frequent, regardless of the technique. Its incidence is related especially to the severity of the appendicitis; patients with complicated appendicitis are more likely to develop an IAA regardless of technique [
16,
17]. In the paediatric population, the incidence of post-operative intra-abdominal abscess after appendectomy for complicated/perforated appendicitis is approximately 24% [
9]. Abscess development is most frequently observed in perforated appendicitis [
7,
10], which represents the main risk factor for this complication to occur [
7‐
9].
Post-operative IAA is associated with [
1,
18] significant morbidity, patient discomfort, prolonged hospital stay and increased cost, often necessitating readmission and repeat intervention [
7,
19‐
22].
The management of IAA remains controversial with different strategies suggested to decrease its incidence: antibiotic prophylaxis [
23,
24], post-operative antibiotic therapy [
25], peritoneal irrigation with saline solution [
26] or suction only of the abscess/purulent liquid without irrigation of the cavity during appendectomy. In the literature, many studies address this topic; however, currently there is no evidence to clearly demonstrate the effectiveness of peritoneal irrigation over suction only. Italian guidelines recommend thorough peritoneal lavage (6–8 L of warm saline) and aspiration to minimize the IAA rate in complicated appendicitis [
27]. The recent WSES (World Society of Emergency Surgery) guidelines report that “Peritoneal irrigation does not have any advantage over suction alone in complicated appendicitis in both adults and children. The performance of irrigation during laparoscopic appendectomy does not seem to prevent the development of IAA and wound infections in neither adults nor paediatric patients”. WSES recommendation is “to perform suction only in complicated appendicitis patients with intra-abdominal collections undergoing laparoscopic appendectomy” [QoE: Moderate; Strength of recommendation: Strong; 1B]) [
28]. The concern regarding irrigation and lavage is that these procedures might help spread the infectious material [
29]. This systematic review of the literature aims to evaluate the available data on comparative studies regarding peritoneal irrigation and suction vs suction only when performing appendectomy for complicated appendicitis.
Discussion
This systematic review with meta-analysis has shown similarity in terms of outcome between the use of peritoneal lavage and suction-only during appendectomy for complicated appendicitis. In particular, our review does not demonstrate a statistical difference in terms of intra-abdominal post-operative abscess, reoperation for abscess, wound infection and hospital stay, between peritoneal irrigation and suction-only of purulent material, in patient underwent appendectomy for complicated appendicitis, laparoscopically or open. Only exception was a lower operative time in suction-only group.
Acute appendicitis is one of the most common gastrointestinal-related diseases, and appendectomy represents one of the most frequently performed abdominal surgical procedures.
Unless appendectomy is considered a safe procedure, it presents an innate risk of complications.
Post-appendectomy complications reported are intra-abdominal abscess (IAA), wound infection, wound dehiscence, small bowel obstruction and bleeding. IAA occurs in 2.2% of cases after laparoscopic appendectomy. In more complicated appendicitis cases, there is a higher risk of development of a post-operative abscess [
7,
10]. Perforated appendicitis was recognized as an independent risk factor for development of post-operative IAA [
7‐
9]. Other risk factors are diabetes mellitus, young and old age [
8], obesity and peritoneal irrigation [
6,
8]. Despite the use of the laparoscopic approach [
2], post-operative complications still present with a high incidence rate [
61,
62]. The management of IAA remains controversial with different strategies suggested to decrease its incidence. Peritoneal irrigation, first described by Torek in 1903, received great emphasis as a procedure to reduce abscess occurrence. Many studies have been conducted since then, comparing peritoneal irrigation versus suction of peritoneal abscess without lavage, leading to an important heterogeneity of findings and opinions.
We performed a systematic review, according to PRISMA guidelines, comparing seventeen studies with case data for peritoneal irrigation and suction vs suction-only of the abdominal cavity during appendectomy. Analysis included the paediatric population, the adult population and combined age groups. Both laparoscopic and open procedures were included. Occurrence of intra-abdominal abscess, reoperation for abscess, operation time, length of stay and wound infections were assessed.
Analysis of the literature revealed early studies which seemed to show the efficiency of peritoneal irrigation in reducing post-operative abscess [
10], especially in adult populations. The success of irrigation was thought to be related to bacterial load dilution. More recent studies suggest the ineffectiveness of irrigation in reducing post-operative IAA [
6,
8,
10] as the effect of bacterial load dilution appears to be temporary. Some current evidence indeed suggests that peritoneal irrigation may increase the incidence of abscess formation in perforated appendicitis [
10,
55].
In 2013, St. Peter defined three mechanisms potentially responsible for the ineffectiveness of peritoneal irrigation:
1.
bacteria adhere to the peritoneal mesothelial cells, so irrigations do not decrease the microorganism load on the peritoneum;
2.
irrigation may cause diffuse or remote bacterial inoculation, thus spreading the pollution [
8];
3.
irrigation may dilute mediators of phagocytosis as opsonic proteins and immunoglobulins.
Nevertheless, many surgeons continue to employ peritoneal lavage during appendectomy [
63].
A minority of authors only found that peritoneal irrigation could have some benefit in reducing post-operative IAA incidence [
51,
60]. Some authors found that peritoneal irrigation seems to increase the risk of developing post-operative IAA after appendectomy, independently from a laparoscopic or open approach [
6,
8].
Some authors reported differences between irrigation and suction and suction only, especially in children, in favour of suction only [
10,
38]. One study was interrupted due to an excess risk of developing an abscess in the lavage group compared to suction-only group [
55].
Most of the analysed studies did not find statistical differences between suction-only versus peritoneal irrigation and suction to prevent post-operative IAA after appendectomy for perforated appendicitis [
8].
Many articles have been published around the use of peritoneal irrigation and suction versus suction only during appendectomy. In this wide and heterogeneous panorama, no articles succeeded in defining whether irrigation is an effective method to reduce post-operative IAA. Only a few studies suggested that peritoneal irrigation could have some benefit in reducing the incidence of post-operative IAA [
51,
60], and the other papers reported that peritoneal irrigation seemed to increase the risk of post-operative IAA after appendectomy, regardless of a laparoscopic or open procedure [
6,
8]. Some authors also reported a prolonged operation time in the lavage group.
In this systematic review, we analysed 17 selected studies (9 RCTs and 8 CCTs) with 5315 patients (2532 irrigation and suction vs 2783 irrigation).
We have identified primary (post-operative IAA and reoperation) and secondary outcomes (operative time, length of hospitalization, wound infections).
Regarding primary outcome, in both laparoscopic and open appendectomy, in children and adult population, we found no statistical difference in terms of onset of intra-abdominal abscess, although the rate of this complication appeared to be lower in patients who underwent suction only.
Same results for reoperation for post-operative abscess: a lower rate was registered in patients who underwent suction only; however, the results were not statistically significant, in both open and laparoscopic groups.
For secondary outcome, in the open and laparoscopic groups, operative time was reported as significantly lower in patients who underwent suction only.
In the open and laparoscopic group, length of hospital stay was lower in patients who underwent peritoneal irrigation and suction, but the result was not statistically significant.
In the open and laparoscopic group, the rates of wound infection were lower in patients who underwent suction only, although the result was not statistically significant. However, the analysis of different age groups shows contrasting results. In the paediatric group, peritoneal irrigation and suction were associated with fewer complications, while in the adult and mixed group suction only seemed to be safer.
Unfortunately, these publications are not homogeneous as some of them analyse a laparoscopic approach only, other just open procedure or other both of them. The population studied were also different: some articles analyse paediatric population, instead other adult population or both.
The risk of bias in the RCTs was assessed using methods described in the Cochrane Handbook for Systematic Reviews of Interventions and the Risk of Bias In Non-randomized Studies of Interventions (ROBINS-I) assessment tool.
The risk of bias was reported as low to moderate.
According to ROBINS-I tool, three studies were assessed as low risk of overall bias, while five were determined to have a moderate risk. Regarding bias due to confounding, four studies were evaluated as having a low risk, four studies seemed to have a moderate risk of bias due to confounding. Regarding bias in classification of the interventions, all studies had a low risk. Outcomes were clearly defined and measures were not influenced by knowledge of the intervention received. The methods of outcome assessment were comparable across intervention groups, and no systematic errors were detected. Analysing bias due to missing data, three reviews were assessed as moderate risk.
This systematic review and meta-analysis has failed to demonstrate the superiority of employing intra-operative peritoneal irrigation and suction over suction-only to reduce the rate of post-operative intra-abdominal abscess after appendectomy (RR 1.23, 95% CI 0.73–2.07; I2 = 72%). Furthermore, the absolute number of abscesses was fewer in suction-only groups both in the overall procedure group (6.91%, 175/2532, in lavage group vs 4.67%, 130/2783, in suction group) and in laparoscopic procedures (6.73%, 131/1947, in lavage group vs 4.57%, 104/2274, in suction group). The subgroup analysis based on patient age yielded the same results.
Operation time (RR 6.10, 95% CI 3.34–8.78; I2 = 39%) and rate of reoperation (RR 6.62, 95% CI 4.49–8.75; I2 = 37%) were significantly lower in patients who underwent suction only.
In the paediatric populations, the lavage groups showed some advantage with regard to the incidence of wound infection for laparoscopic appendectomy, although this was not statistically significant (MD 1.16, 95% CI 0.56–2.38; I2 = 71%).
Overall no statistical significance was found in the defined primary and secondary outcomes, except for operation time, between peritoneal irrigation and suction group compared to suction only in treating complicated appendicitis. Clinical advantages, which were not statistically significant, for reduced incidence of post-operative intra-abdominal abscess and reoperation for abscess, operative time and wound infection were detected in the suction-only groups.