Correction: Blood-brain barrier-associated pericytes internalize and clear aggregated amyloid-β42 by LRP1-dependent apolipoprotein E isoform-specific mechanism
verfasst von:
Qingyi Ma, Zhen Zhao, Abhay P. Sagare, Yingxi Wu, Min Wang, Nelly Chuqui Owens, Philip B. Verghese, Joachim Herz, David M. Holtzman, Berislav V. Zlokovic
After publication of this work [1], an error was noticed in Fig. 4B.
Fig. 4
LRP1 mediates clearance of aggregated Cy3-Aβ42 by mouse pericytes. a-b Multiphoton/confocal laser scanning microscopy of multi-spot glass slides coated with Cy3-Aβ42 without cells (a), and with primary mouse brain pericytes cultured for 5 days in the presence of NI-IgG or anti-LRP1, after si.Lrp1 silencing compared to scrambled si.Control, and with RAP or vehicle (b). Scale bar, 50 μm. c Quantification of Cy3-Aβ42 relative signal intensity on multi-spot slides after 5 days without cells (open bar on the left) and with pericytes in the presence of vehicle (control), NI-IgG and anti-LRP1, after silencing with scrambled si.Control or si.Lrp1, and in the presence of RAP. N = 4 independent cultures (biological replicates, see Methods); mean ± s.e.m.; p < 0.05 by One-way ANOVA followed by Bonferroni post-hoc test. d Quantification of TUNEL+ pericyte cell death at 3 and 7 days after seeding on multi-spot glass slides coated with Cy3-Aβ42 in the presence and absence of NI-IgG and anti-LRP1, and after si.Lrp1 silencing or si.Ctrl as in (b). N = 3 independent cultures per group; mean ± s.e.m.; p < 0.05 by One-way ANOVA followed by Bonferroni post-hoc test
×
In the Cell Tracker column shown below on the left, an incorrect representative image for Cell Tracker was used for the NI-IgG condition (non-immune IgG control). The error in this representative Cell Tracker image likely occurred when updates were made to the representative images in this figure during preparation of the manuscript by mistake. The Cy3-Aβ image for this NI-IgG condition was correct and does not need to be replaced.
This error only pertains to the incorrect representative Cell Tracker NI-IgG image in Fig. 4B, and does not affect any of the analysis or conclusions presented in the paper, as all quantifications were based on the Cy3-Aβ (red) signal intensity representing Aβ cleared by the cells under different conditions.
After carefully going back through all the raw data, we found and selected the exact correct Cell Tracker image for this particular NI-IgG condition shown in Fig. 4B corresponding to the Cy3-Aβ (red) signal.
Anzeige
We would appreciate to publish erratum and replace incorrect representative Cell Tracker image as shown on the left side below (as published) with the correct representative Cell Tracker image for this particular NI-IgG condition shown below on the right side.
The correct version of the entire Fig. 4 with the correct Cell tracker image for panel 4B NI-IgG representative image is shown below. Nothing else in this figure needs to be changed. The analysis or conclusions presented in the paper remain the same as is, because the quantifications were based on the remaining Cy3-Aβ (red) signal intensity representing Aβ uncleared by the cells.
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Correction: Blood-brain barrier-associated pericytes internalize and clear aggregated amyloid-β42 by LRP1-dependent apolipoprotein E isoform-specific mechanism
verfasst von
Qingyi Ma Zhen Zhao Abhay P. Sagare Yingxi Wu Min Wang Nelly Chuqui Owens Philip B. Verghese Joachim Herz David M. Holtzman Berislav V. Zlokovic
Patienten, die von Ärztinnen behandelt werden, dürfen offenbar auf bessere Therapieergebnisse hoffen als Patienten von Ärzten. Besonders gilt das offenbar für weibliche Kranke, wie eine Studie zeigt.
Akuter Schwindel stellt oft eine diagnostische Herausforderung dar. Wie nützlich dabei eine MRT ist, hat eine Studie aus Finnland untersucht. Immerhin einer von sechs Patienten wurde mit akutem ischämischem Schlaganfall diagnostiziert.
Wenn unter einer medikamentösen Hochdrucktherapie der diastolische Blutdruck in den Keller geht, steigt das Risiko für schwere kardiovaskuläre Ereignisse: Darauf deutet eine Sekundäranalyse der SPRINT-Studie hin.
Ist die Tau-Last noch gering, scheint der Vorteil von Lecanemab besonders groß zu sein. Und beginnen Erkrankte verzögert mit der Behandlung, erreichen sie nicht mehr die kognitive Leistung wie bei einem früheren Start. Darauf deuten neue Analysen der Phase-3-Studie Clarity AD.
Update Neurologie
Bestellen Sie unseren Fach-Newsletterund bleiben Sie gut informiert.
