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Pathology & Oncology Research

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13.07.2016 | Original Article

Low Expression LncRNA TUBA4B is a Poor Predictor of Prognosis and Regulates Cell Proliferation in Non-Small Cell Lung Cancer

verfasst von: Jian Chen, Lijuan Hu, Junjun Wang, Fan Zhang, Jie Chen, Gang Xu, Yumin Wang, Qinshi Pan

Erschienen in: Pathology & Oncology Research | Ausgabe 2/2017

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Abstract

We aimed to unveil the clinical roles and biological function of lncRNA TUBA4B in on-small cell lung cancer (NSCLC). The relative expression level of TUBA4B was estimated by qPCR in 114 pairs of NSCLC and NT samples and the relation of TUBA4B to clinical data of NSCLC patients was analyzed. We found TUBA4B was lower expressed in NSCLC and five cell lines. The lower expression of TUBA4B was remarkably correlated with advanced TNM stage and lymph node metastasis and served as a predictor for overall survival of NSCLC. After overexpression of TUBA4B, cell proliferation ability of A549 and NCI-H1299 remarkably decreased. Our study ascertained low expression TUBA4B in NSCLC tissue, cell lines and is a poor predictor for prognosis and can regulate cell proliferation in NSCLC.

Jemal A, Murray T, Ward E, Samuels A, Tiwari RC, Ghafoor A, Feuer EJ, Thun MJ. Cancer statistics, 2005. CA: a cancer journal for clinicians. 2005;55:10–30.

Gridelli C, Rossi A, Maione P (2003) Treatment of non-small-cell lung cancer: state of the art and development of new biologic agents. Oncogene 22:6629–6638CrossRefPubMed

Stewart DJ (2010) Tumor and host factors that may limit efficacy of chemotherapy in non-small cell and small cell lung cancer. Crit Rev Oncol Hematol 75:173–234CrossRefPubMedPubMedCentral

Chen CH, Lai JM, Chou TY, Chen CY, Su LJ, Lee YC, Cheng TS, Hong YR, Chou CK, Whang-Peng J, Wu YC, Huang CY. VEGFA upregulates FLJ10540 and modulates migration and invasion of lung cancer via PI3K/AKT pathway. PLoS One 2009;4:e5052.

Ogawa E, Takenaka K, Katakura H, Adachi M, Otake Y, Toda Y, Kotani H, Manabe T, Wada H, Tanaka F (2008) Perimembrane aurora-a expression is a significant prognostic factor in correlation with proliferative activity in non-small-cell lung cancer (NSCLC). Ann Surg Oncol 15:547–554CrossRefPubMed

Rachet B, Woods LM, Mitry E, Riga M, Cooper N, Quinn MJ, Steward J, Brenner H, Esteve J, Sullivan R, Coleman MP. Cancer survival in England and Wales at the end of the twentieth century. Br J Cancer 2008;99 Suppl 1:S2–10.

Fu X, Ravindranath L, Tran N, Petrovics G, Srivastava S (2006) Regulation of apoptosis by a prostate-specific and prostate cancer-associated noncoding gene, PCGEM1. DNA Cell Biol 25:135–141CrossRefPubMed

Gupta RA, Shah N, Wang KC, Kim J, Horlings HM, Wong DJ, Tsai MC, Hung T, Argani P, Rinn JL, Wang Y, Brzoska P, Kong B, Li R, West RB, van de Vijver MJ, Sukumar S, Chang HY (2010) Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Nature 464:1071–1076CrossRefPubMedPubMedCentral

Zhang H, Chen Z, Wang X, Huang Z, He Z, Chen Y. Long non-coding RNA: a new player in cancer. J Hematol Oncol 2013;6:37.

10.

Hauptman N, Glavac D (2013) Long non-coding RNA in cancer. Int J Mol Sci 14:4655–4669CrossRefPubMedPubMedCentral

11.

Chen G, Wang Z, Wang D, Qiu C, Liu M, Chen X, Zhang Q, Yan G, Cui Q (2013) LncRNADisease: a database for long-non-coding RNA-associated diseases. Nucleic Acids Res 41:D983–D986CrossRefPubMed

12.

Gibb EA, Brown CJ, Lam WL (2011) The functional role of long non-coding RNA in human carcinomas. Mol Cancer 10:–38

13.

Ji P, Diederichs S, Wang W, Boing S, Metzger R, Schneider PM, Tidow N, Brandt B, Buerger H, Bulk E, Thomas M, Berdel WE, Serve H, Muller-Tidow C (2003) MALAT-1, a novel noncoding RNA, and thymosin beta4 predict metastasis and survival in early-stage non-small cell lung cancer. Oncogene 22:8031–8041CrossRefPubMed

14.

Thai P, Statt S, Chen CH, Liang E, Campbell C, Wu R (2013) Characterization of a novel long noncoding RNA, SCAL1, induced by cigarette smoke and elevated in lung cancer cell lines. Am J Respir Cell Mol Biol 49:204–211CrossRefPubMedPubMedCentral

15.

Yang Y, Li H, Hou S, Hu B, Liu J, Wang J (2013) The noncoding RNA expression profile and the effect of lncRNA AK126698 on cisplatin resistance in non-small-cell lung cancer cell. PLoS One 8:–e65309

16.

Han L, Kong R, Yin DD, Zhang EB, TP X, De W, Shu YQ (2013) Low expression of long noncoding RNA GAS6-AS1 predicts a poor prognosis in patients with NSCLC. Med Oncol 30:–694

17.

Ren S, Peng Z, Mao JH, Yu Y, Yin C, Gao X, Cui Z, Zhang J, Yi K, Xu W, Chen C, Wang F, Guo X, Lu J, Yang J, Wei M, Tian Z, Guan Y, Tang L, Xu C, Wang L, Gao X, Tian W, Wang J, Yang H, Wang J, Sun Y (2012) RNA-seq analysis of prostate cancer in the Chinese population identifies recurrent gene fusions, cancer-associated long noncoding RNAs and aberrant alternative splicings. Cell Res 22:806–821CrossRefPubMedPubMedCentral

18.

Drobnik W, Liebisch G, Biederer C, Tr mbach B, Rogler G, Muller P, Schmitz G (1999) Growth and cell cycle abnormalities of fibroblasts from Tangier disease patients. Arterioscler Thromb Vasc Biol 19:28–38CrossRefPubMed

19.

Wang KC, Chang HY (2011) Molecular mechanisms of long noncoding RNAs. Mol Cell 43:904–914CrossRefPubMedPubMedCentral

20.

Khachane AN, Harrison PM. Mining mammalian transcript data for functional long non-coding RNAs. PLoS One 2010;5:e10316.

21.

Loh YH, Wu Q, Chew JL, Vega VB, Zhang W, Chen X, Bourque G, George J, Leong B, Liu J, Wong KY, Sung KW, Lee CW, Zhao XD, Chiu KP, Lipovich L, Kuznetsov VA, Robson P, Stanton LW, Wei CL, Ruan Y, Lim B, Ng HH (2006) The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells. Nat Genet 38:431–440CrossRefPubMed

22.

Mattick JS, Gagen MJ (2001) The evolution of controlled multitasked gene networks: the role of introns and other noncoding RNAs in the development of complex organisms. Mol Biol Evol 18:1611–1630CrossRefPubMed

23.

Mattick JS (2010) Linc-ing long noncoding RNAs and enhancer function. Dev Cell 19:485–486CrossRefPubMed

24.

Orom UA, Derrien T, Beringer M, Gumireddy K, Gardini A, Bussotti G, Lai F, Zytnicki M, Notredame C, Huang Q, Guigo R, Shiekhattar R (2010) Long noncoding RNAs with enhancer-like function in human cells. Cell 143:46–58CrossRefPubMedPubMedCentral

Titel: Low Expression LncRNA TUBA4B is a Poor Predictor of Prognosis and Regulates Cell Proliferation in Non-Small Cell Lung Cancer
verfasst von: Jian Chen
Lijuan Hu
Junjun Wang
Fan Zhang
Jie Chen
Gang Xu
Yumin Wang
Qinshi Pan
Publikationsdatum: 13.07.2016
Verlag: Springer Netherlands
Erschienen in: Pathology & Oncology Research / Ausgabe 2/2017
Print ISSN: 1219-4956
Elektronische ISSN: 1532-2807
DOI: https://doi.org/10.1007/s12253-016-0089-y

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Low Expression LncRNA TUBA4B is a Poor Predictor of Prognosis and Regulates Cell Proliferation in Non-Small Cell Lung Cancer

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