Erschienen in:
01.03.2000 | Meeting abstract
Low frequency of p16
INK4a
promoter methylation in mammary carcinomas as revealed by positive display of methylated sites
verfasst von:
R Lilischkis, H Kneitz, H Kreipe
Erschienen in:
Breast Cancer Research
|
Sonderheft 1/2000
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Excerpt
Promoter methylation represents an important mechanism for silencing gene expression in higher eukaryotes. In order to study methylation of the promoter of the tumour suppressor p16 INK4a, we developed a fast and simple method that in contrast to previous studies relies on the positive display of methylated sites (PDM). The method is based on bisulphite treatment of DNA, PCR-amplification of the modified DNA, and restriction digest of de novo created restriction sites to positively display DNA methylation in a background of unmethylated DNA. Since methylated as well as unmethylated DNA is amplified, information on the proportion of both is provided. …