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19.11.2016 | Original Article

Low-level laser irradiation modulates brain-derived neurotrophic factor mRNA transcription through calcium-dependent activation of the ERK/CREB pathway

verfasst von: Xiaodong Yan, Juanfang Liu, Zhengping Zhang, Wenhao Li, Siguo Sun, Jian Zhao, Xin Dong, Jixian Qian, Honghui Sun

Erschienen in: Lasers in Medical Science | Ausgabe 1/2017

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Abstract

Low-level laser (LLL) irradiation has been reported to promote neuronal differentiation, but the mechanism remains unclear. Brain-derived neurotrophic factor (BDNF) has been confirmed to be one of the most important neurotrophic factors because it is critical for the differentiation and survival of neurons during development. Thus, this study aimed to investigate the effects of LLL irradiation on Bdnf messenger RNA (mRNA) transcription and the molecular pathway involved in LLL-induced Bdnf mRNA transcription in cultured dorsal root ganglion neurons (DRGNs) using Ca²⁺ imaging, pharmacological detections, RNA interference, immunocytochemistry assay, Western blot, and qPCR analysis. We show here that LLL induced increases in the [Ca²⁺]_i level, Bdnf mRNA transcription, cAMP-response element-binding protein (CREB) phosphorylation, and extracellular signal-regulated kinase (ERK) phosphorylation, mediated by Ca²⁺ release via inositol triphosphate receptor (IP3R)-sensitive calcium (Ca²⁺) stores. Blockade of Ca²⁺ increase suppressed Bdnf mRNA transcription, CREB phosphorylation, and ERK phosphorylation. Downregulation of phosphorylated (p)-CREB reduced Bdnf mRNA transcription triggered by LLL. Furthermore, blockade of ERK using PD98059 inhibitor reduced p-CREB and Bdnf mRNA transcription induced by LLL. Taken together, these findings establish the Ca²⁺-ERK-CREB cascade as a potential signaling pathway involved in LLL-induced Bdnf mRNA transcription. To our knowledge, this is the first report of the mechanisms of Ca²⁺-dependent Bdnf mRNA transcription triggered by LLL. These findings may help further explore the complex molecular signaling networks in LLL-triggered nerve regeneration in vivo and may also provide experimental evidence for the development of LLL for clinical applications.

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Titel: Low-level laser irradiation modulates brain-derived neurotrophic factor mRNA transcription through calcium-dependent activation of the ERK/CREB pathway
verfasst von: Xiaodong Yan
Juanfang Liu
Zhengping Zhang
Wenhao Li
Siguo Sun
Jian Zhao
Xin Dong
Jixian Qian
Honghui Sun
Publikationsdatum: 19.11.2016
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 1/2017
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-016-2099-0

Springer Medizin

Abstract

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