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01.10.2014 | Original Article

Low-level laser therapy for osteonecrotic lesions: effects on osteoblasts treated with zoledronic acid

verfasst von: Fernanda Gonçalves. Basso, Ana Paula Silveira. Turrioni, Diana Gabiela Soares, Vanderlei Salvador Bagnato, Josimeri Hebling, Carlos Alberto de Souza Costa

Erschienen in: Supportive Care in Cancer | Ausgabe 10/2014

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Abstract

Purpose

Clinical studies have shown that low-level laser therapy (LLLT) can improve local tissue healing of bisphosphonate-induced osteonecrosis of the jaw. However, the effects of laser irradiation on bisphosphonate-treated osteoblasts have not been completely elucidated.

Methods

Human osteoblasts were cultured in plain culture medium (DMEM). After 48 h, plain DMEM was replaced by DMEM with no fetal bovine serum, for a 24-h incubation followed by addition of zoledronic acid (5 μM) for additional 48 h. Cells were subjected to LLLT (InGaAsP; 780 ± 3 nm; 0.025 W) at 0.5, 1.5, 3, 5, and 7 J/cm², three times every 24 h. Cell viability, total protein production, alkaline phosphatase activity (ALP), mineral nodule formation, gene expression of collagen type I and ALP, and cell morphology were evaluated.

Results

LLLT at 0.5 J/cm² increased cell viability of cultured osteoblasts. ALP activity and gene expression, in addition to mineral nodule formation and Col-I gene expression, were not increased by LLLT. LLLT applied to ZA-treated cells increased Col-I expression at 0.5, 1.5, and 3 J/cm² but did not improve any other cell activity assessed.

Conclusion

LLLT showed limited effects on bisphosphonate-treated osteoblasts.

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Titel: Low-level laser therapy for osteonecrotic lesions: effects on osteoblasts treated with zoledronic acid
verfasst von: Fernanda Gonçalves. Basso
Ana Paula Silveira. Turrioni
Diana Gabiela Soares
Vanderlei Salvador Bagnato
Josimeri Hebling
Carlos Alberto de Souza Costa
Publikationsdatum: 01.10.2014
Verlag: Springer Berlin Heidelberg
Erschienen in: Supportive Care in Cancer / Ausgabe 10/2014
Print ISSN: 0941-4355
Elektronische ISSN: 1433-7339
DOI: https://doi.org/10.1007/s00520-014-2267-3

Springer Medizin

Low-level laser therapy for osteonecrotic lesions: effects on osteoblasts treated with zoledronic acid