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15.10.2017 | Original Article

MAGE-A11 expression contributes to cisplatin resistance in head and neck cancer

verfasst von: Stefan Hartmann, Leonie Zwick, Mario J. J. Scheurer, Andreas R. Fuchs, Roman C. Brands, Axel Seher, Hartmut Böhm, Alexander C. Kübler, Urs D. A. Müller-Richter

Erschienen in: Clinical Oral Investigations | Ausgabe 3/2018

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Abstract

Objective

The objective of this study is to investigate the roles of melanoma-associated antigens (MAGEs) in the cisplatin treatment of head and neck cancer.

Materials and methods

We assessed the efficacy of cisplatin in a set of four head and neck cancer cell lines using a crystal violet assay. The MAGE-A expression in all cell lines was measured with RT-qPCR. The correlation between MAGE-A expression and cisplatin efficacy was investigated using Spearman’s correlation analysis. Furthermore, we established a cell line with stable overexpression of MAGE-A11 and determined influence on proliferation, cisplatin efficacy and cell apoptosis. In this cell line, the effects of cisplatin were assessed using either crystal violet assays or flow cytometry (Annexin V).

Results

For MAGE-A11, we observed the highest correlation (r = 1.000, p = 0.0417) with low cisplatin efficacy. Stable overexpression of MAGE-A11 resulted in no changes in proliferation, but in lower cisplatin cytotoxicity and lower rates of apoptosis. Also, mouse double minute 2 homolog (MDM2) expression was induced by MAGE-A11 overexpression.

Conclusion

We provide evidence that MAGE-A11 expression contributes to cisplatin resistance in head and neck cancer.

Clinical relevance

Our study underscores the negative predictive role of MAGE-A11 expression in head and neck cancer.

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Titel: MAGE-A11 expression contributes to cisplatin resistance in head and neck cancer
verfasst von: Stefan Hartmann
Leonie Zwick
Mario J. J. Scheurer
Andreas R. Fuchs
Roman C. Brands
Axel Seher
Hartmut Böhm
Alexander C. Kübler
Urs D. A. Müller-Richter
Publikationsdatum: 15.10.2017
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 3/2018
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-017-2242-8

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MAGE-A11 expression contributes to cisplatin resistance in head and neck cancer