History
MRC is a rare congenital entity. Earlier literature consisting of case reports are mainly from Japan [
1,
8‐
11]. However, Asarch
et al. (USA) compiled six cases reported as early as 1979 [
7]. Nagore
et al. described a case series of five patients from Spain in 1998 [
6]. In recent decades, cases have been reported from other parts of the world as well (also see Table
1).
Table 1
Reports with two or more than two cases of median raphe cyst
| 1979 | 6 | USA |
Otsuka et al. [ 11] (genitoperineal raphe) | 1990 | 160 | Japan |
| 1992 | 2 | USA |
Pellicé i Vilalta and Luelmo i Aguilar [ 13] | 1997 | 2 | Spain |
| 1998 | 5 | Spain |
| 1998 | 3 | Japan |
| 2000 | 2 | Spain |
| 2001 | 2 | Italy |
| 2009 | 2 | Spain |
| 2009 | 2 | India |
| 2012 | 55 | Taiwan |
| 2016 | 23 | Japan |
Navalon-Monllor et al. [ 4] | 2017 | 28 | Spain |
| 2017 | 2 | India |
Several terms have been used in the past to describe the condition. It includes genitoperineal cyst of median raphe [
19,
20], mucoid cyst of penis [
21], apocrine cystadenoma/hydrocystoma of penis [
22,
23] and epidermoid cysts [
24]. A cyst close to the meatus has been referred to as parameatal cyst [
1,
25,
26]. However, in the view of authors, the term that could be synonymous to MRC, is genitoperineal cyst of median raphe. It’s still debatable if all these entities are actually same or different [
15]. One fact that unites these conditions is that they all reflect developmental defect in male genitalia during embryogenesis. Mucoid cyst may result from faulty closure of penoscrotoperineal raphe with sequestration of ectopic urethral mucosa [
21]. An apocrine cystadenoma/hydrocystoma has bluish hue on clinical examination, while pathological details would reveal focal areas of decapitation secretion in epithelial lining, along with a myoepithelial layer [
22,
23]. An epidermoid cyst may result due abnormal closure of the median raphe during embryogenesis or, rarely, following mechanical implantation in acquired cases [
27]. A parameatal cyst may arise due to obstruction of paraurethral duct or anomalous fusion of urethra [
28].
Clinical presentation
The cyst is most commonly noticed in the first decade of life; however, because of the condition being asymptomatic, the patient often presents during second to third decades of life. Our patient’s mother first noticed the cyst when he was at the age of 3, but the patient came for medical advice at the age of 29 years, when he was planning to get married. Shao
et al. mentioned the mean age of presentation to be 26.7 years with a bimodal age distribution at approximately 1–10 years and 21–40 years [
5]. Navalón-Monllor
et al. found a slightly lower mean age (24.6 years) with similar bimodal age characteristics, having higher presentation during the first and third decades of life [
4] (also see Table
2).
Table 2
Individual cases of median raphe cyst reported with age < 1 year and > 60 years
| 2 | 3 months and 6 months |
| 1 | 4 months |
| 1 | 4 months |
| 1 | 8 months |
| 1 | 9 months |
| 1 | 62 years |
| 1 | 65 years |
| 1 | 67 years |
| 1 | 68 years |
| 1 | 76 years |
Our patient was asymptomatic, and the cyst had ceased to grow for more than a decade. MRCs are mostly asymptomatic [
3‐
5,
7,
31] and grow proportionately with body size [
33]. Though asymptomatic, MRC has the potential to cause psychological embarrassment and discomfort [
31]. Parameatal cysts are more likely to give rise to symptoms of urinary obstruction [
4,
5,
34]. These symptoms are limited to dysuria, urinary frequency, or deflected urinary stream [
3,
16,
35]. Pain, if present, is an indication of infection [
4,
5,
12]. Rarely, hematuria, hematospermia [
5], and difficulty in sexual function [
34,
36] may be presenting complaints. A cyst near the anal region is mistaken for hemorrhoids [
37]. The most common reason for consultation is aesthetic [
4,
15]. The more distal the cyst and earlier the age at presentation, the greater the chances of it being symptomatic [
5]. A case of appearance of MRC in the shaft of the penis following intense, prolonged sexual intercourse has been described [
38].
The cyst is generally solitary [
3‐
5], sometimes double [
3], and rarely presents as a chain of cystic swellings along the median raphe [
4,
10,
33,
39]. The chain of cysts gives a cordlike appearance and has been described as canaliform MRCs [
17,
29,
40]. Another variant is the presence of cysts within the raphe canal, which is an elongated tract along the median raphe [
41‐
44]. Coexistence of a cystic-type lesion and canaliform variant in different regions has also been reported [
37]. Videodermatoscopy can help confirm the presence of a true canal [
43]. The cyst is always obvious on the surface; however, a case of MRC was reported from the United Kingdom in which MRC presented as nonvisible palpable swelling that was confirmed on USG and magnetic resonance imaging (MRI) [
45]. Another variation in presentation could be the beginning of the condition with a single lesion with subsequent appearance of more cysts [
46]. The cyst very rarely may have central umbilication, giving rise to differential diagnosis of molluscum contagiosum [
47].
The cysts are generally translucent, and the penile shaft is the most common location [
3‐
5]. However, many authors have separately defined parameatal urethral cysts, whose numbers exceed those of cysts in any other location [
3,
28,
48,
49]. Consideration of parameatal cyst as a separate condition or a form of MRC needs more discussion in the scientific community. We tend to agree making parameatal the most common variant of MRC. The cyst may have a bluish hue [
6], which is more a characteristic of cystadenoma/hydrocystoma of the penis. Pigmented cysts appearing as brown-black in color due to presence of melanocytes and melanin pigment in the epithelial lining have also been reported [
10,
46,
50]. Multiple areas are rarely involved. The majority of the cysts do not reach size in excess of 1 cm [
4,
5]. However, Matsuyama
et al. [
3] reported that about 70% of the patients in their study had size less than 0.5 cm. Scrotal cysts tend to be larger than cysts elsewhere [
5]. In our patient, the size of the cyst was about 1 cm, which made it larger than the mean observed size of these cysts. MRCs with sizes in excess of 2 cm have also been reported [
16,
32,
50]. A comparative analysis of a few clinical features of MRC as reported in three large reviews is presented in Table
3. MRC has to be differentiated from a number of conditions. A differential diagnosis by location is given in Table
4.
Table 3
Clinical characteristics of median raphe cyst in three large reviews
No. of cases | 55a | 23 | 28 |
Cyst size range | 0.2–2.1 cm | 0.1 to > 1 cm | 0.5–3.5 cm |
Mean size of cyst | 0.88 cm | NA | 1.1 cm |
Cyst location |
Parameatal | 19 (33.9) |
b
| 8 (28) |
Glans penis | 4 (7.1) | – | – |
Penile shaft | 24 (42.9) | 11 (47.8) | 10 (36) |
Scrotum/perineum | 2 (3.6) | 2 (8.7) | 2 (7) |
Prepuce | 7 (12.7) | – | 3 (11) |
Multiple areas | | 4 (17.4) | 2 (7) |
Corona/balanic frenulum | | 6 (26.1) | 3 (11) |
Symptoms |
Asymptomatic | 40 (72.7) | 19 (82.6) | 22 (79) |
Symptomatic | 15 (27.3) | 4 (17.3) | 4 (21) |
Table 4
Differential diagnosis of median raphe cyst by location
Glans penis | Urethral diverticulum, capillary/cavernous hemangioma, glomus tumor, leiomyoma [ 51] |
Shaft | Steatocystoma, molluscum contagiosum, trichilemmal cyst [ 52], dermoid and epidermoid cysts, lipoma |
Scrotum | Steatocystoma, calcinosis cutis |
Perineum | Cowper gland cyst, lipoma, epidermoid cyst |
Perianal | Hemorrhoids, perianal polyp, pilonidal cyst, hidradenitis, teratomas [ 53] |
Pathogenesis
A debate on the pathogenesis of MRC has also seen varied views. The cyst may represent an embryological developmental anomaly of male genitalia or a defect in closure of the median raphe. The genital tubercle, two urethral folds and the scrotal swellings, give rise to male external genitalia. The scrotal swellings fuse in the midline to form the scrotum, which leaves a permanent surface marking in the form of median raphe. An incomplete closure of the genital or urethral fold gives rise to epithelial rest. The rest may develop into either a cyst or a canal, depending on the presence or absence of an opening on skin surface [
54]. A cyst can appear even after primary closure from split-off outgrowths of embryonic epithelium [
54]. A theory that also finds support is “tissue trapping,” in which epithelial rests may get buried during midline fusion and evolve into a cyst or canal [
12]. Autologous transplant of skin tissue specimens has shown the development of cysts at the transplant sites, lending credibility to the tissue-trapping theory [
55]. Shiraki, on the basis of a study of nine cases of parameatal cyst of glans penis, proposed the occlusion of paraurethral ducts resulting in cyst formation as an explanation [
48]. A congenital obliteration of these ducts hampered the physiological drainage and led to development of cysts [
48]. Infection and trauma are acquired contributory factors in the obstruction [
49]. Light and electron microscopic findings of Otsuka
et al. support this theory [
1]. Cole and Helwig proposed an alternative, stating that these cysts could be the result of sequestered ectopic periurethral glands of Littre [
21]. However, strong evidence in support of this explanation is still lacking.
Histopathology/IHC
The cyst in our patient was unilocular, which is the condition most frequently encountered. However, multilocular cysts can also be seen [
16]. The pathogenetic mechanism and type of tissue trapped explain the tissue lining [
6,
7]. Trapping of the proximal and distal urethra would result in pseudostratified and stratified squamous epithelial lining, respectively. If the periurethral glands are trapped, they will form a glandular lining of cyst. Shoa
et al. published an extensive report based on histopathological findings in 55 cases of MRCs [
5]. They classified MRCs into four groups, depending on the type of epithelial lining of the cyst wall:
1.
Urethral: Lined by pseudostratified columnar epithelium, such as the urothelium
2.
Epidermoid: Lined by squamous stratified epithelium
3.
Glandular: Lined by urethral epithelium with interspersed glandular structure
4.
Mixed: Lined by more than one type of epithelium, such as urethral epithelium with squamous metaplasia or mucinous cells, or all the three coexisting
We would like to extend this classification and add two more categories: ciliated and pigmented. The ciliated type is characterized by the presence of ciliated cells interspersed with pseudostratified [
16,
26,
47,
56] or columnar [
31,
57,
58] epithelium. The pigmented variant appears brown-black in color due to the presence of melanocytes and melanin pigment in the epithelial lining [
10,
46,
50]. Pigment granules can become evident with Fontana-Masson stain [
50]. Though case reports on these two groups always claimed them to be rare findings, we would like to challenge this claim. Ciliated cysts have been reported more frequently than glandular variant (see Table
4). The epidermoid type, too, is uncommonly reported (see Table
4) [
5], but it has never been referred to as rare. Some authors attribute ciliated cysts to an additional abnormality of embryological development [
47], whereas others refer to it as a result of metaplastic changes in the urothelium [
58]. In our extensive literature search, we could only find three cases of pigmented variant [
10,
46,
50], which in our view makes them truly rare.
Unal
et al. [
56], in their compilation of cases of ciliated cysts, mentioned six cases, including their own, as documented in the literature. Perhaps they missed the case reported by Navarro
et al. in 2009 [
16] and the one reported by Amaranathan
et al. in 2013 [
57] (also see Table
4). We extend this list and include these two cases. We further include our patient’s case as well, which also showed ciliated, pseudostratified epithelium, thus taking the number of total reported cases to nine. In Table
5, we compile the histopathological findings of 2 large reviews of MRC and 29 individual case reports/series documented separately as one group.
Table 5
Histopathological findings in 2 large reviews and a third group comprising 29 individual case reports
Number of cases | 56a | 28 | 29 |
Type of epithelium |
Urethral | 31 (55.4) | 15 (54) | Total: 12/29 (41.3) |
Epidermoid | 3 (5.4) | 2 (7) | Total: 3/29 (10.3) |
Glandular | 2 (3.4) | Total: 3/29 (10.3) |
Mixed | 20 (35.7) | 11 (39) | Total: 11/29 (37.9) |
Rare variants |
Ciliated | – | – | |
Pigmented | – | – | |
IHC has been undertaken in several case reports (Table
6). The most consistent staining is seen with cytokeratin 7, strongly indicative of urethral origin of the cyst (Table
6). Positive cytokeratin 13 lends further support. Carcinoembryonic antigen positivity has also been regularly reported, except by Persec
et al. [
60]. Cytokeratin 20 and smooth muscle actin results remain negative.
Table 6
IHC results in various case reports
CK7 | + | + | + | + | + | + | + |
CK13 | + | + | | | | | |
CK20 | – | – | – | – | – | – | – |
CEA | + | + | + | + | – | + | + |
Anti-S100 | | – | + | – | – | | + |
SMA | | | – | – | – | | – |
Desmin | | | – | | | | |
EMA | | + | + | + | + | | |
HMFG-1 | | – | | | | | |
CAM5.2 | + | | | | | | |
GCDFP-15 | | | | – | | | + |
Complications
The cyst may be secondarily infected and manifest as pain, tenderness, erythema, or pus discharge. About 16% of patients reported by Shao
et al. had infected cysts [
5]. The most common organism found is
Neisseria gonorrhoeae [
63,
64]. Infection with
Trichomonas vaginalis can also occur, but it is rare [
65]. However, these are case reports of infection of the median raphe rather MRC.
Staphylococcus aureus infecting MRC of the scrotum and penis has been noted in old case reports [
54,
66]. Infection of MRC has been uncommonly documented in recent decades. The culture of the cystic content can help confirm the infective organism. Infection is generally a phenomenon noticed after sexual intercourse. The cyst may also be traumatized by other means and become infected. Urinary obstruction, if due to a parameatal cyst, does not lead to urethritis. MRCs never communicate with the urethra; however, recently, a case of an epidermoid variant traversing the corpus cavernosum has been reported [
67]. Another case of MRC in the scrotum was found to mimic a serous tumor and was associated with cryptorchidism [
68]. A few very unusual cases of MRC reported in the literature are compiled in Table
7.
Table 7
A few very unusual cases of median raphe cyst reported in the literature
Bhasin et al. | Giant median raphe prepuceal cyst in an elderly male. | |
Takahashi et al. | Congenital median raphe cysts: coexistence of cystic lesions and canal-like lesions. | |
Sharkey et al. | Postcoital appearance of a median raphe cyst. | |
Yu et al. | A case of epidermoid median raphe cyst traversing the corpora cavernosa. | |
Hara et al. | Median raphe cyst in the scrotum, mimicking a serous borderline tumor, associated with cryptorchidism after orchiopexy. | |
Treatment
Spontaneous resolution has been reported [
3,
9,
28]. Observation is another option when the cyst is small and the child is asymptomatic [
12]. Because the cyst mostly remains symptom-free, some patients deny any active treatment [
6]. If left untreated, the cyst may rupture on its own and heal uneventfully [
12]. Aspiration of the cyst is associated with recurrence [
5,
33,
48]. Marsupialization or unroofing is not recommended, because it may lead to gaping sinus [
48]. However, marsupialization has been found to be effective in treating canals [
41]. Median raphe canals have also been treated with incision followed by electrodessication [
7]. Excision followed by primary closure remains the treatment of choice [
3‐
5,
7] and provides cosmetically acceptable results. When the cyst lies in the prepuce, circumcision can also be performed [
32,
57]. A giant cyst hanging at the frenulum has been treated with excision and repair by frenuloplasty [
49].
Excision is associated with excellent results, with no evidence of recurrence in 6 months [
36], 1 year [
34,
45], and 4 years [
56] of follow-up. In a case series with six patients, Asarch
et al. noted recurrence in one of the patients after 5 years, and the cyst was reexcised with no subsequent recurrence [
7]. One of the patients of Shao
et al. developed a fistula following excision [
5]. In a retrospective study spanning 14 years, Matsuyama
et al. did not identify a single case of recurrence following treatment [
3].