Figure 2
Detection of a panel of keratins (green signal, A-L, I
2
-L
2
), keratin 14 (red signal, A, A
1
, E, E
1
, I, I
1
, I
3
), keratin 8 (red signal, B, B
1
, F, F
1
, J, J
1
, J
3
), keratin 19 (red signal C, C
1
, G, G
1
, K, K
1
, K
3
) and vimentin (red signal, D, D
1
, H, H
1
, L, L
1
, L
3
) in keratinocytes cultured on 3 T3 feeder cells in presence of BLM melanoma cells (A-D, A
1
- D
1
), of highly pigmented neonatal melanocytes (HPM, E-H, E
1
-H
1
) and of neural crest stem cells (NCSC, I-L, I
1–3
-L
1–3
). The co-localization of signal has been verified by the measurement of fluorescence profile (I3-L3). The figures show that BLM and NCSC have a similar stimulatory effect on the expression of all the studied markers. Scale bar denotes 50 μm.