Background
Methods
Tissue supply
CD34+ cell culture
Meso scale discovery (MSD) assay
HUVEC cultures
In vitro Matrigel tube formation assay
Total RNA extraction
Microarray experiments and gene expression analysis
Quantitative RT-PCR
Statistical analysis
Results
Secretion of pro-inflammatory and pro-angiogenic cytokines by CD34+ cells
The effect of metformin on in vitro angiogenic function of CD34+ cell-derived CM
Metformin drives a gene expression profile consistent with an anti-inflammatory action under euglycemia and euglycemia-hypoxia
Gene name | Gene symbol | FC | Family |
---|---|---|---|
Asp (abnormal spindle) homolog, microcephaly associated (Drosophila) | ASPM | −1.52 | Other |
Complement component 3 | C3 | −1.51 | Peptidase |
Chemokine (C–C motif) ligand 2 | CCL2 | −1.68 | Cytokine |
Chemokine (C–C motif) ligand 5 | CCL5 | −1.81 | Cytokine |
CD24 molecule | CD24 | −1.61 | Other |
Centromere protein E, 312 kDa | CENPE | −1.51 | Other |
C-type lectin domain family 2, member D | CLEC2D | −1.59 | Transmembrane receptor |
Chymase 1, mast cell | CMA1 | 1.58 | Peptidase |
Cathepsin L1 | CTSL1 | −1.50 | Peptidase |
Glomulin, FKBP associated protein | GLMN | −1.62 | Other |
Heparanase | HPSE | −1.60 | Enzyme |
Immunoglobulin heavy constant epsilon | IGHE | 1.57 | Other |
Interleukin 18 receptor 1 | IL-18R1 | −1.56 | Transmembrane receptor |
Interleukin 1, alpha | IL-1α | −3.66 | Cytokine |
Interleukin 26 | IL-26 | 1.53 | Cytokine |
Interleukin 36, gamma | IL-1F9 | −1.56 | Cytokine |
Interleukin 6 (interferon, beta 2)a
| IL-6 | −2.21 | Cytokine |
Interleukin 8a
| IL-8 | −1.55 | Cytokine |
Integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) | ITGB3 | −1.51 | Transmembrane receptor |
Met proto-oncogene (hepatocyte growth factor receptor) | MET | −1.63 | Kinase |
Antigen identified by monoclonal antibody Ki-67 | MKI67 | −1.61 | Other |
Nuclear receptor corepressor 2 | NCOR2 | 1.77 | Transcription regulator |
Pro-platelet basic protein (chemokine (C–X–C motif) ligand 7) | PPBP | −1.69 | Cytokine |
S100 calcium binding protein A8 | S100A8 | 1.71 | Other |
Serpin peptidase inhibitor, clade B (ovalbumin), member 2 | SERPINB2 | −4.62 | Other |
Sclerostin | SOST | 1.56 | Other |
SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) | SPC25 | −1.50 | Other |
STEAP family member 4 | STEAP4 | 1.90 | Oxidoreductase |
Thrombospondin 1 | THBS1 | −1.50 | Other |
Tumour necrosis factor, alpha-induced protein 6a
| TNFAIP6 | −2.36 | Other |
Metformin drives a gene expression profile consistent with improved cell survival in CD34+ cells under hyperglycemia
Metformin drives a gene expression profile consistent with pro-angiogenic action in CD34+ cells under hyperglycemia with hypoxia
Gene name | Gene symbol |
p value | FC |
---|---|---|---|
Non-SMC condensin I complex, subunit H | NCAPH | 1.46E−09 | 2.24 |
Polo-like kinase 1 | PLK1 | 1.54E−09 | 4.71 |
Cyclin A2 | CCNA2 | 1.94E−09 | 2.57 |
Serine/arginine-rich splicing factor 4 | SRSF4 | 1.15E−08 | −1.73 |
Protein regulator of cytokinesis 1 | PRC1 | 1.17E−08 | 2.17 |
TIMP metallopeptidase inhibitor 3 | TIMP3 | 1.24E−08 | 2.14 |
Lysine (K)-specific demethylase 5D | KDM5D | 2.59E−08 | 1.86 |
TPX2, microtubule-associated, homolog (Xenopus laevis) | TPX2 | 6.63E−08 | 2.64 |
Cell division cycle 20 homolog (S. cerevisiae) | CDC20 | 8.42E−08 | 3.08 |
Olfactory receptor, family 2, subfamily L, member 13 | OR2L13 | 9.20E−08 | −1.93 |
Prohibitin | PHB | 9.25E−08 | 1.99 |
ZW10 interactor | ZWINT | 9.82E−08 | 1.93 |
X-ray repair complementing defective repair in Chinese hamster cells 2 | XRCC2 | 1.44E−07 | 1.61 |
Brain expressed, X-linked 4 | BEX4 | 1.47E−07 | −1.76 |
Protein kinase, membrane associated tyrosine/threonine 1 | PKMYT1 | 1.61E−07 | 2.14 |
Golgin A8 family, member B | GOLGA8B | 1.62E−07 | −1.64 |
Holliday junction recognition protein | HJURP | 1.67E−07 | 2.55 |
S100 calcium binding protein A8 | S100A8 | 2.10E−07 | −5.24 |
TIMP metallopeptidase inhibitor 1 | TIMP1 | 6.34E−06 | 2.36 |
Chemokine (C–X–C motif) ligand 10 | CXCL10 | 2.04E−02 | 1.89 |
Gene name | Gene symbol |
p value | FC |
---|---|---|---|
Serine/arginine-rich splicing factor 4 | SRSF4 | 3.32E−09 | 1.87 |
Brain expressed, X-linked 4 | BEX4 | 1.43E−07 | 1.76 |
Dihydrouridine synthase 4-like (S. cerevisiae) | DUS4L | 2.41E−07 | 1.60 |
Mitochondrial ribosomal protein S21 | MRPS21 | 2.99E−07 | 1.52 |
Endoplasmic reticulum protein 29 | ERP29 | 6.47E−07 | −1.74 |
Prohibitin | PHB | 1.40E−06 | −1.67 |
Chromosome 11 open reading frame 58 | C11orf58 | 1.48E−06 | 1.91 |
NOP14 nucleolar protein homolog (yeast) | NOP14 | 1.50E−06 | 1.52 |
Deoxynucleotidyltransferase, terminal, interacting protein 1 | DNTTIP1 | 1.97E−06 | 1.91 |
Family with sequence similarity 98, member A | FAM98A | 2.03E−06 | 1.52 |
Dynactin 3 (p22) | DCTN3 | 2.25E−06 | −2.14 |
Solute carrier family 25, member 32 | SLC25A32 | 2.47E−06 | 2.02 |
Heterogeneous nuclear ribonucleoprotein H3 (2H9) | HNRNPH3 | 4.21E−06 | 1.72 |
Glycerol-3-phosphate acyltransferase, mitochondrial | GPAM | 4.31E−06 | −10.76 |
Zinc finger protein 391 | ZNF391 | 4.55E−06 | 1.59 |
Chromosome 11 open reading frame 51 | C11orf51 | 4.61E−06 | 2.36 |
USO1 vesicle docking protein homolog (yeast) | USO1 | 4.64E−06 | 1.55 |
Transducer of ERBB2, 2 | TOB2 | 1.22E−05 | 4.48 |
TIMP metallopeptidase inhibitor 1 | TIMP1 | 3.90E−04 | −1.68 |
Chemokine (C–X–C motif) ligand 10 | CXCL10 | 1.28E−02 | −2.01 |