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Erschienen in: European Journal of Clinical Microbiology & Infectious Diseases 1/2005

01.01.2005 | Concise Article

Method for detection of respiratory viruses in the sputa of patients with cystic fibrosis

verfasst von: G. Punch, M. W. Syrmis, B. R. Rose, C. Harbour, P. T. P. Bye, M. D. Nissen, M. R. Elkins, T. P. Sloots

Erschienen in: European Journal of Clinical Microbiology & Infectious Diseases | Ausgabe 1/2005

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Abstract

Since the role of respiratory viruses in lung exacerbations of patients with cystic fibrosis has been hampered by the difficulty of detecting viruses in viscous sputum specimens, a multiplex reverse transcriptase PCR (RT-PCR) assay combined with colorimetric amplicon detection was tested for the identification of seven common respiratory viruses in the sputa of cystic fibrosis patients. Of 52 sputa from 38 patients, 12 (23%) samples from 12 patients were positive for a respiratory virus (4 for influenza B, 3 for parainfluenza 1, 3 for influenza A and 2 for respiratory syncytial virus). These results suggest that the RT-PCR method carried out on sputum may provide a convenient means of investigating the role of virus infection in lung exacerbations of cystic fibrosis patients.
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Metadaten
Titel
Method for detection of respiratory viruses in the sputa of patients with cystic fibrosis
verfasst von
G. Punch
M. W. Syrmis
B. R. Rose
C. Harbour
P. T. P. Bye
M. D. Nissen
M. R. Elkins
T. P. Sloots
Publikationsdatum
01.01.2005
Erschienen in
European Journal of Clinical Microbiology & Infectious Diseases / Ausgabe 1/2005
Print ISSN: 0934-9723
Elektronische ISSN: 1435-4373
DOI
https://doi.org/10.1007/s10096-004-1273-7

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