nach oben

Erschienen in:

28.01.2019 | Diagnostic Neuroradiology

Micro-computed tomography: a new diagnostic tool in postmortem assessment of brain anatomy in small fetuses

verfasst von: Sophie Lombardi, Elisa Scola, Davide Ippolito, Vanessa Zambelli, Giovanni Botta, Serena Cuttin, Fabio Triulzi, Claudio M. Lombardi

Erschienen in: Neuroradiology | Ausgabe 7/2019

Einloggen, um Zugang zu erhalten

Abstract

Purpose

The aim of our study was to evaluate the postmortem micro-CT anatomy of early fetal human fetal brains, either in situ or isolated.

Methods

We studied 12 ex vivo specimens, 9 whole human fetuses (9–18 GW), and 3 isolated samples (16–26 GW).

Specimens were fixed in formalin, then immersed in Lugol solution. Images were evaluated by two neuroradiologists. The depiction of CNS structures was defined based on the comparison between micro-CT images and a reference histologic anatomical Atlas of human brain development.

Results

Micro-CT provided informative high-resolution brain images in all cases, with the exception of one case (9 weeks) due to advanced maceration. All major CNS structures (i.e., brain hemispheres, layering, ventricles, germinal neuroepithelium, basal ganglia, corpus callosum, major cranial nerves, and structures of the head and neck) were recognizable.

Conclusions

Micro-CT imaging of the early fetal brain is feasible and provides high-quality images that correlate with the histological Atlas of the human brain, offering multiplanar and volumetric images that can be stored and shared for clinical, teaching, and research purposes.

Nur mit Berechtigung zugänglich

Engels AC, Joyeux L, Brantner C, De Keersmaecker B, De Catte L, Baud D, Deprest J, Van Mieghem T (2016) Sonographic detection of central nervous system defects in the first trimester of pregnancy. Prenat Diagn 36:266–273. https://doi.org/10.1002/pd.4770 CrossRefPubMed

Syngelaki A, Chelemen T, Dagklis T, Allan L, Nicolaides KH (2011) Challenges in the diagnosis of fetal non-chromosomal abnormalities at 11-13 weeks. Prenat Diagn 31:90–102. https://doi.org/10.1002/pd.2642 CrossRefPubMed

Katorza E, Achiron R (2012) Early pregnancy scanning for fetal anomalies--the new standard? Clin Obstet Gynecol 55:199–216. https://doi.org/10.1097/GRF.0b013e3182446ae9 CrossRefPubMed

Volpe P, Contro E, Fanelli T, Muto B, Pilu G, Gentile M (2016) Appearance of fetal posterior fossa at 11-14 weeks in fetuses with Dandy-Walker malformation or chromosomal anomalies. Ultrasound Obstet Gynecol 47:720–725. https://doi.org/10.1002/uog.14883 CrossRefPubMed

Contro E, Volpe P, De Musso F, Muto B, Ghi T, De Robertis V, Pilu G (2014) Open fourth ventricle prior to 20 weeks’ gestation: a benign finding? Ultrasound Obstet Gynecol 43:154–158. https://doi.org/10.1002/uog.13227 CrossRefPubMed

Arthurs OJ, Thayyil S, Pauliah SS, Jacques TS, Chong WK, Gunny R, Saunders D, Addison S, Lally P, Cady E, Jones R, Norman W, Scott R, Robertson NJ, Wade A, Chitty L, Taylor AM, Sebire NJ, Group MRIAS (MaRIAS) C (2015) Diagnostic accuracy and limitations of post-mortem MRI for neurological abnormalities in fetuses and children. Clin Radiol 70:872–880. https://doi.org/10.1016/j.crad.2015.04.008 CrossRefPubMed

Gilbert-Barness E, Spicer DE, Steffensen TS (2014) Handbook of pediatric autopsy pathology. Springer

Connolly AJ, Finkbeiner WE, Ursell PC, Richard L, Davis M (2016) Autopsy pathology: a manual and atlas. Elsevier

Lombardi CM, Zambelli V, Botta G, Moltrasio F, Cattoretti G, Lucchini V, Fesslova V, Cuttin MS (2014) Postmortem microcomputed tomography (micro-CT) of small fetuses and hearts. Ultrasound Obstet Gynecol 44:600–609. https://doi.org/10.1002/uog.13330 CrossRefPubMed

10.

Thayyil S, Sebire NJ, Chitty LS, Wade A, Chong W, Olsen O, Gunny RS, Offiah AC, Owens CM, Saunders DE, Scott RJ, Jones R, Norman W, Addison S, Bainbridge A, Cady EB, De VE, Robertson NJ, Taylor AM (2013) Post-mortem MRI versus conventional autopsy in fetuses and children: a prospective validation study. Lancet 382:223–233. https://doi.org/10.1016/S0140-6736(13)60134-8 CrossRefPubMed

11.

Hutchinson JC, Arthurs OJ, Ashworth MT, Ramsey AT, Mifsud W, Lombardi CM, Sebire NJ (2016) Clinical utility of postmortem microcomputed tomography of the fetal heart: diagnostic imaging vs macroscopic dissection. Ultrasound Obstet Gynecol 47:58–64. https://doi.org/10.1002/uog.15764 CrossRefPubMed

12.

Lewis C, Hill M, Arthurs O, Hutchinson C, Chitty L, Sebire N (2018) Factors affecting uptake of postmortem examination in the prenatal, perinatal and paediatric setting. BJOG An Int J Obstet Gynaecol 125:172–181. https://doi.org/10.1111/1471-0528.14600 CrossRef

13.

Thayyil S, Cleary JO, Sebire NJ, Scott RJ, Chong K, Gunny R, Owens CM, Olsen OE, Offiah AC, Parks HG, Chitty LS, Price AN, Yousry TA, Robertson NJ, Lythgoe MF, Taylor AM (2009) Post-mortem examination of human fetuses: a comparison of whole-body high-field MRI at 9.4 T with conventional MRI and invasive autopsy. Lancet (London, England) 374:467–475. https://doi.org/10.1016/S0140-6736(09)60913-2 CrossRef

14.

Scola E, Conte G, Palumbo G, Avignone S, Cinnante CM, Boito S, Persico N, Rizzuti T, Triulzi F (2018) High resolution post-mortem MRI of non-fixed in situ foetal brain in the second trimester of gestation: Normal foetal brain development. Eur Radiol 28:363–371. https://doi.org/10.1007/s00330-017-4965-y CrossRefPubMed

15.

Kang X, Cannie MM, Arthurs OJ, Segers V, Fourneau C, Bevilacqua E, Cos Sanchez T, Sebire NJ, Jani JC (2017) Post-mortem whole-body magnetic resonance imaging of human fetuses: a comparison of 3-T vs. 1.5-T MR imaging with classical autopsy. Eur Radiol 27:3542–3553. https://doi.org/10.1007/s00330-016-4725-4 CrossRefPubMed

16.

Metscher BD (2009) MicroCT for developmental biology: a versatile tool for high-contrast 3D imaging at histological resolutions. Dev Dyn 238:632–640. https://doi.org/10.1002/dvdy.21857 CrossRefPubMed

17.

Hutchinson JC, Barrett H, Ramsey AT, Haig IG, Guy A, Sebire NJ, Arthurs OJ (2016) Virtual pathological examination of the human fetal kidney using micro-CT. Ultrasound Obstet Gynecol 48:663–665. https://doi.org/10.1002/uog.15859 CrossRefPubMed

18.

Hutchinson JC, Kang X, Shelmerdine SC, Segers V, Lombardi CM, Cannie MM, Sebire NJ, Jani JC, Arthurs OJ (2018) Postmortem microfocus computed tomography for early gestation fetuses: a validation study against conventional autopsy. Am J Obstet Gynecol 218:445.e1–445.e12. https://doi.org/10.1016/j.ajog.2018.01.040 CrossRef

19.

Thayyil S (2011) Less invasive autopsy: an evidenced based approach. Arch Dis Child 96:681–687. https://doi.org/10.1136/adc.2009.165704 CrossRefPubMed

20.

De Crespigny A, Bou-reslan H, Nishimura MC, Phillips H, Richard A, Carano D, Arceuil HED (2009) 3D microCT of the postmortem brain. J Neurosci Methods 171:207–213. https://doi.org/10.1016/j.jneumeth.2008.03.006.3D CrossRef

21.

Dobrivojević M, Bohaček I, Erjavec I, Gorup D, Gajović S (2013) Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents. Croat Med J 54:3–11. https://doi.org/10.3325/cmj.2013.54.3 CrossRefPubMedPubMedCentral

22.

Gignac PM, Kley NJ, Clarke JA, Colbert MW, Morhardt AC, Cerio D, Cost IN, Cox PG, Daza JD, Early CM, Echols MS, Henkelman RM, Herdina AN, Holliday CM, Li Z, Mahlow K, Merchant S, Müller J, Orsbon CP, Paluh DJ, Thies ML, Tsai HP, Witmer LM (2016) Diffusible iodine-based contrast-enhanced computed tomography (diceCT): an emerging tool for rapid, high-resolution, 3-D imaging of metazoan soft tissues. J Anat 228:889–909. https://doi.org/10.1111/joa.12449 CrossRefPubMedPubMedCentral

23.

Degenhardt K, Wright AC, Horng D, Padmanabhan A, Epstein JA (2010) Rapid 3D phenotyping of cardiovascular development in mouse embryos by micro-CT with iodine staining. Circ Cardiovasc Imaging 3:314–322. https://doi.org/10.1161/CIRCIMAGING.109.918482 CrossRefPubMedPubMedCentral

24.

Li Z, Clarke JA, Ketcham RA, Colbert MW, Yan F (2015) An investigation of the efficacy and mechanism of contrast-enhanced X-ray computed tomography utilizing iodine for large specimens through experimental and simulation approaches. BMC Physiol 15(5). https://doi.org/10.1186/s12899-015-0019-3

25.

Kostovic I, Vasung L (2009) Insights from in vitro fetal magnetic resonance imaging of cerebral development. Semin Perinatol 33:220–233. https://doi.org/10.1053/j.semperi.2009.04.003 CrossRefPubMed

Titel: Micro-computed tomography: a new diagnostic tool in postmortem assessment of brain anatomy in small fetuses
verfasst von: Sophie Lombardi
Elisa Scola
Davide Ippolito
Vanessa Zambelli
Giovanni Botta
Serena Cuttin
Fabio Triulzi
Claudio M. Lombardi
Publikationsdatum: 28.01.2019
Verlag: Springer Berlin Heidelberg
Erschienen in: Neuroradiology / Ausgabe 7/2019
Print ISSN: 0028-3940
Elektronische ISSN: 1432-1920
DOI: https://doi.org/10.1007/s00234-019-02168-2

Leitlinien kompakt für die Neurologie

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Neurologie

Akuter Schwindel: Wann lohnt sich eine MRT?

28.04.2024 Schwindel Nachrichten

Akuter Schwindel stellt oft eine diagnostische Herausforderung dar. Wie nützlich dabei eine MRT ist, hat eine Studie aus Finnland untersucht. Immerhin einer von sechs Patienten wurde mit akutem ischämischem Schlaganfall diagnostiziert.

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

25.04.2024 Hypotonie Nachrichten

Wenn unter einer medikamentösen Hochdrucktherapie der diastolische Blutdruck in den Keller geht, steigt das Risiko für schwere kardiovaskuläre Ereignisse: Darauf deutet eine Sekundäranalyse der SPRINT-Studie hin.

Frühe Alzheimertherapie lohnt sich

25.04.2024 AAN-Jahrestagung 2024 Nachrichten

Ist die Tau-Last noch gering, scheint der Vorteil von Lecanemab besonders groß zu sein. Und beginnen Erkrankte verzögert mit der Behandlung, erreichen sie nicht mehr die kognitive Leistung wie bei einem früheren Start. Darauf deuten neue Analysen der Phase-3-Studie Clarity AD.

Viel Bewegung in der Parkinsonforschung

25.04.2024 Parkinson-Krankheit Nachrichten

Neue arznei- und zellbasierte Ansätze, Frühdiagnose mit Bewegungssensoren, Rückenmarkstimulation gegen Gehblockaden – in der Parkinsonforschung tut sich einiges. Auf dem Deutschen Parkinsonkongress ging es auch viel um technische Innovationen.

Update Neurologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Abstract

Purpose

Methods

Results

Conclusions

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 7/2019

Resting fMRI as an alternative for task-based fMRI for language lateralization in temporal lobe epilepsy patients: a study using independent component analysis

Dependency of the blood oxygen level dependent-response to hyperoxic challenges on the order of gas administration in intracranial malignancies

Preoperative evaluation of tumour consistency in pituitary macroadenomas: a machine learning-based histogram analysis on conventional T2-weighted MRI

Dual-energy CT angiography-derived virtual non-contrast images for follow-up of patients with surgically clipped aneurysms: a retrospective study

Altered brain diffusion tensor imaging indices in adolescents with the Fontan palliation

Subtraction CT angiography in the follow-up of treated cerebral aneurysms