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Erschienen in: Clinical Oral Investigations 2/2019

10.05.2018 | Original Article

Micro-CT evaluation of different final irrigation protocols on the removal of hard-tissue debris from isthmus-containing mesial root of mandibular molars

verfasst von: Emmanuel João Nogueira Leal Silva, Carla Rodrigues Carvalho, Felipe Gonçalves Belladonna, Marina Carvalho Prado, Ricardo Tadeu Lopes, Gustavo De-Deus, Edson Jorge Lima Moreira

Erschienen in: Clinical Oral Investigations | Ausgabe 2/2019

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Abstract

Objectives

This study aimed to compare four final irrigation protocols (passive ultrasonic irrigation [PUI], EndoVac, Self-Adjusting File [SAF] and EasyClean) on the removal of accumulated hard-tissue debris (AHTD) from mesial canals of mandibular molars through microcomputed tomographic (micro-CT) analysis.

Materials and methods

Forty mesial roots of mandibular molars presenting isthmuses type I or III were scanned in a micro-CT device and instrumented up to Reciproc R40 instrument. After the completion of canal preparations, root canals of each group were submitted to a final rinse using 20 mL of solution (16 mL of 5.25% NaOCl and 4 mL of 17% EDTA) in a total time of 5 min according to one of the four final irrigation protocols (n = 10): PUI, EndoVac, SAF and EasyClean operated at reciprocating motion. The sample was scanned again after canal preparation and after the use of the final irrigation protocols, and the registered data sets were examined to evaluate the percentage of AHTD. Data were statistically compared using the Tukey test with a significance level set at 5%.

Results

All groups presented a decrease on the accumulation of hard-tissue debris after the use of the final irrigation protocols (P < 0.05). No significant differences in the removal of AHTD were observed among the final irrigation protocols (P > 0.05).

Conclusions

All final irrigation protocols showed the same effectiveness in the removal of AHTD. None of them was able to render mesial canals of mandibular molars completely free from packed debris.

Clinical relevance

This study highlighted that all final irrigation protocols (PUI, EndoVac, SAF, and EasyClean) promoted a similar removal of AHTD. However, none of the final irrigation protocols was able to render mesial canals of mandibular molars completely free from packed debris.
Literatur
1.
Zurück zum Zitat Metzger Z, Solomonov M, Kfir A (2013) The role of mechanical instrumentation in the cleaning of root canals. Endod Top 29:87–109CrossRef Metzger Z, Solomonov M, Kfir A (2013) The role of mechanical instrumentation in the cleaning of root canals. Endod Top 29:87–109CrossRef
2.
Zurück zum Zitat Paqué F, Laib A, Gautschi H, Zehnder M (2009) Hard-tissue debris accumulation analysis by high-resolution computed tomography scans. J Endod 35:1044–1047CrossRef Paqué F, Laib A, Gautschi H, Zehnder M (2009) Hard-tissue debris accumulation analysis by high-resolution computed tomography scans. J Endod 35:1044–1047CrossRef
3.
Zurück zum Zitat De-Deus G, Marins J, Silva EJ, Souza E, Belladonna FG, Reis C et al (2015) Accumulated hard tissue debris produced during reciprocating and rotary nickel-titanium canal preparation. J Endod 41:676–681CrossRef De-Deus G, Marins J, Silva EJ, Souza E, Belladonna FG, Reis C et al (2015) Accumulated hard tissue debris produced during reciprocating and rotary nickel-titanium canal preparation. J Endod 41:676–681CrossRef
5.
Zurück zum Zitat Zuolo ML, Zaia AA, Belladonna FG, Silva EJNL, Souza EM, Versiani MA et al. (2017) Micro-CT assessment of the shaping ability of four root canal instrumentation systems in oval-shaped canals. Int Endod J 51:564–71CrossRef Zuolo ML, Zaia AA, Belladonna FG, Silva EJNL, Souza EM, Versiani MA et al. (2017) Micro-CT assessment of the shaping ability of four root canal instrumentation systems in oval-shaped canals. Int Endod J 51:564–71CrossRef
6.
Zurück zum Zitat Siqueira JF Jr (2003) Microbial causes of endodontic flare-ups. Int Endod J 36:453–463CrossRef Siqueira JF Jr (2003) Microbial causes of endodontic flare-ups. Int Endod J 36:453–463CrossRef
7.
Zurück zum Zitat De-Deus G, Reis C, Beznos D, de Abranches AM, Coutinho-Filho T, Paciornik S (2008) Limited ability of three commonly used thermoplasticized gutta-percha techniques in filling oval-shaped canals. J Endod 34:1401–1405CrossRef De-Deus G, Reis C, Beznos D, de Abranches AM, Coutinho-Filho T, Paciornik S (2008) Limited ability of three commonly used thermoplasticized gutta-percha techniques in filling oval-shaped canals. J Endod 34:1401–1405CrossRef
8.
Zurück zum Zitat Nusstein JM (2015) Sonic and ultrasonic irrigation. In: Bettina B (ed) Endodontic irrigation: chemical disinfection of the root canal system. Springer, Cham, pp 173–198CrossRef Nusstein JM (2015) Sonic and ultrasonic irrigation. In: Bettina B (ed) Endodontic irrigation: chemical disinfection of the root canal system. Springer, Cham, pp 173–198CrossRef
9.
Zurück zum Zitat Nielsen BA, Baumgartner JC (2007) Comparison of the EndoVac system to needle irrigation of root canals. J Endod 33:611–615CrossRef Nielsen BA, Baumgartner JC (2007) Comparison of the EndoVac system to needle irrigation of root canals. J Endod 33:611–615CrossRef
10.
Zurück zum Zitat Metzger Z, Teperovich E, Zary R, Cohen R, Hof R (2010) The self-adjusting file (SAF). Part 1: respecting the root canal anatomy—a new concept of endodontic files and its implementation. J Endod 36:679–690CrossRef Metzger Z, Teperovich E, Zary R, Cohen R, Hof R (2010) The self-adjusting file (SAF). Part 1: respecting the root canal anatomy—a new concept of endodontic files and its implementation. J Endod 36:679–690CrossRef
11.
Zurück zum Zitat Andrade-Junior CV, Batista RM, Marceliano-Alves M, Alves F, Silva EJNL (2016) Efficacy of a new activation device in irrigant penetration into simulated lateral canals. Eur Endod J 1:2–5 Andrade-Junior CV, Batista RM, Marceliano-Alves M, Alves F, Silva EJNL (2016) Efficacy of a new activation device in irrigant penetration into simulated lateral canals. Eur Endod J 1:2–5
12.
Zurück zum Zitat Kato AS, Cunha RS, da Silveira Bueno CE, Pelegrine RA, Fontana CE, de Martin AS (2016) Investigation of the efficacy of passive ultrasonic irrigation versus irrigation with reciprocating activation: an environmental scanning electron microscopic study. J Endod 42:659–663CrossRef Kato AS, Cunha RS, da Silveira Bueno CE, Pelegrine RA, Fontana CE, de Martin AS (2016) Investigation of the efficacy of passive ultrasonic irrigation versus irrigation with reciprocating activation: an environmental scanning electron microscopic study. J Endod 42:659–663CrossRef
13.
Zurück zum Zitat Schneider SW (1971) A comparison of canal preparations in straight and curved root canals. Oral Surg Oral Med Oral Pathol 32:271–275CrossRef Schneider SW (1971) A comparison of canal preparations in straight and curved root canals. Oral Surg Oral Med Oral Pathol 32:271–275CrossRef
14.
Zurück zum Zitat Fan B, Pan Y, Gao Y, Fang F, Wu Q, Gutmann JL (2010) Three-dimensional morphologic analysis of isthmuses in the mesial roots of mandibular molars. J Endod 36:1866–1869CrossRef Fan B, Pan Y, Gao Y, Fang F, Wu Q, Gutmann JL (2010) Three-dimensional morphologic analysis of isthmuses in the mesial roots of mandibular molars. J Endod 36:1866–1869CrossRef
15.
Zurück zum Zitat Susin L, Liu Y, Yoon JC, Parente JM, Loushine RJ, Ricucci D, Bryan T, Weller RN, Pashley DH, Tay FR (2010) Canal and isthmus debridement efficacies of two irrigant agitation techniques in a closed system. Int Endod J 43:1077–1090CrossRef Susin L, Liu Y, Yoon JC, Parente JM, Loushine RJ, Ricucci D, Bryan T, Weller RN, Pashley DH, Tay FR (2010) Canal and isthmus debridement efficacies of two irrigant agitation techniques in a closed system. Int Endod J 43:1077–1090CrossRef
16.
Zurück zum Zitat Fedorov A, Beichel R, Kalpathy-Cramer J, Finet J, Fillion-Robin JC, Pujol S, Bauer C, Jennings D, Fennessy F, Sonka M, Buatti J, Aylward S, Miller JV, Pieper S, Kikinis R (2012) 3D slicer as an image computing platform for the quantitative imaging network. Magn Reson Imaging 30:1323–1324CrossRef Fedorov A, Beichel R, Kalpathy-Cramer J, Finet J, Fillion-Robin JC, Pujol S, Bauer C, Jennings D, Fennessy F, Sonka M, Buatti J, Aylward S, Miller JV, Pieper S, Kikinis R (2012) 3D slicer as an image computing platform for the quantitative imaging network. Magn Reson Imaging 30:1323–1324CrossRef
17.
Zurück zum Zitat Neves AA, Silva EJ, Roter JM, Belladona FG, Alves HD, Lopes RT, Paciornik S, de-Deus GA (2015) Exploiting the potential of free software to evaluate root canal biomechanical preparation outcomes through micro-CT images. Int Endod J 48:1033–1042CrossRef Neves AA, Silva EJ, Roter JM, Belladona FG, Alves HD, Lopes RT, Paciornik S, de-Deus GA (2015) Exploiting the potential of free software to evaluate root canal biomechanical preparation outcomes through micro-CT images. Int Endod J 48:1033–1042CrossRef
18.
Zurück zum Zitat Perez R, Neves AA, Belladonna FG, Silva EJNL, Souza EM, Fidel S, Versiani MA, Lima I, Carvalho C, de-Deus G (2017) Impact of needle insertion depth on the removal of hard-tissue debris. Int Endod J 50:560–568CrossRef Perez R, Neves AA, Belladonna FG, Silva EJNL, Souza EM, Fidel S, Versiani MA, Lima I, Carvalho C, de-Deus G (2017) Impact of needle insertion depth on the removal of hard-tissue debris. Int Endod J 50:560–568CrossRef
19.
Zurück zum Zitat de Pablo OV, Estevez R, Peix Sanchez M, Heilborn C, Cohenca N (2010) Root anatomy and canal configuration of the permanent mandibular first molar: a systematic review. J Endod 36:1919–1931CrossRef de Pablo OV, Estevez R, Peix Sanchez M, Heilborn C, Cohenca N (2010) Root anatomy and canal configuration of the permanent mandibular first molar: a systematic review. J Endod 36:1919–1931CrossRef
20.
Zurück zum Zitat Paragliola R, Franco V, Fabiani C, Mazzoni A, Nato F, Tay FR, Breschi L, Grandini S (2010) Final rinse optimization: influence of different agitation protocols. J Endod 36:282–285CrossRef Paragliola R, Franco V, Fabiani C, Mazzoni A, Nato F, Tay FR, Breschi L, Grandini S (2010) Final rinse optimization: influence of different agitation protocols. J Endod 36:282–285CrossRef
21.
Zurück zum Zitat Siqueira JF Jr, Alves FRF, Versiani MA, Rôças IN, Almeida BM, Neves MA et al (2010) Correlative bacteriologic and micro-computed tomographic analysis of mandibular molar mesial canals prepared by Self-Adjusting File, Reciproc, and Twisted File systems. J Endod 39:1044–1050CrossRef Siqueira JF Jr, Alves FRF, Versiani MA, Rôças IN, Almeida BM, Neves MA et al (2010) Correlative bacteriologic and micro-computed tomographic analysis of mandibular molar mesial canals prepared by Self-Adjusting File, Reciproc, and Twisted File systems. J Endod 39:1044–1050CrossRef
22.
Zurück zum Zitat Ordinola-Zapata R, Bramante CM, Aprecio RM, Handysides R, Jaramillo DE (2014) Biofilm removal by 6% sodium hypochlorite activated by different irrigation techniques. Int Endod J 47:659–666CrossRef Ordinola-Zapata R, Bramante CM, Aprecio RM, Handysides R, Jaramillo DE (2014) Biofilm removal by 6% sodium hypochlorite activated by different irrigation techniques. Int Endod J 47:659–666CrossRef
23.
Zurück zum Zitat Peters OA (2004) Current challenges and concepts in the preparation of root canal systems: a review. J Endod 30:559–567CrossRef Peters OA (2004) Current challenges and concepts in the preparation of root canal systems: a review. J Endod 30:559–567CrossRef
24.
Zurück zum Zitat Versiani MA, Alves FR, Andrade-Junior CV, Marceliano-Alves MF, Provenzano JC, Rôças IN et al (2016) Micro-CT evaluation of the efficacy of hard-tissue removal from the root canal and isthmus area by positive and negative pressure irrigation systems. Int Endod J 49:1079–1087CrossRef Versiani MA, Alves FR, Andrade-Junior CV, Marceliano-Alves MF, Provenzano JC, Rôças IN et al (2016) Micro-CT evaluation of the efficacy of hard-tissue removal from the root canal and isthmus area by positive and negative pressure irrigation systems. Int Endod J 49:1079–1087CrossRef
25.
Zurück zum Zitat van der Sluis LW, Shemesh H, Wu MK, Wesselink PR (2007) An evaluation of the influence of passive ultrasonic irrigation on the seal of root canal fillings. Int Endod J 40:356–361CrossRef van der Sluis LW, Shemesh H, Wu MK, Wesselink PR (2007) An evaluation of the influence of passive ultrasonic irrigation on the seal of root canal fillings. Int Endod J 40:356–361CrossRef
26.
Zurück zum Zitat ElAyouti A, Kiefner P, Hecker H, Chu A, L€ost C, Weiger R (2009) Homogeneity and adaptation of endodontic fillings in root canals with enlarged apical preparation. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 108:e141–e146CrossRef ElAyouti A, Kiefner P, Hecker H, Chu A, L€ost C, Weiger R (2009) Homogeneity and adaptation of endodontic fillings in root canals with enlarged apical preparation. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 108:e141–e146CrossRef
27.
Zurück zum Zitat Brunson M, Heilborn C, Johnson DJ, Cohenca N (2010) Effect of apical preparation size and preparation taper on irrigant volume delivered by using negative pressure irrigation system. J Endod 36:721–724CrossRef Brunson M, Heilborn C, Johnson DJ, Cohenca N (2010) Effect of apical preparation size and preparation taper on irrigant volume delivered by using negative pressure irrigation system. J Endod 36:721–724CrossRef
28.
Zurück zum Zitat Thomas AR, Velmurugan N, Smita S, Jothilatha S (2014) Comparative evaluation of canal isthmus debridement efficacy of modified EndoVac technique with different irrigation systems. J Endod 40:1676–1680CrossRef Thomas AR, Velmurugan N, Smita S, Jothilatha S (2014) Comparative evaluation of canal isthmus debridement efficacy of modified EndoVac technique with different irrigation systems. J Endod 40:1676–1680CrossRef
29.
Zurück zum Zitat Freire LG, Iglecias EF, Cunha RS, Dos Santos M, Gavini G (2015) Micro-computed tomographic evaluation of hard tissue debris removal after different irrigation methods and its influence on the filling of curved canals. J Endod 41:1660–1666CrossRef Freire LG, Iglecias EF, Cunha RS, Dos Santos M, Gavini G (2015) Micro-computed tomographic evaluation of hard tissue debris removal after different irrigation methods and its influence on the filling of curved canals. J Endod 41:1660–1666CrossRef
30.
Zurück zum Zitat Keleş A, Alçin H, Sousa-Neto MD, Versiani MA (2016) Supplementary steps for removing hard tissue debris from isthmus-containing canal systems. J Endod 42:1677–1682CrossRef Keleş A, Alçin H, Sousa-Neto MD, Versiani MA (2016) Supplementary steps for removing hard tissue debris from isthmus-containing canal systems. J Endod 42:1677–1682CrossRef
31.
Zurück zum Zitat Topçuoglu HS, Akti A, Düzgün S, Ceyhanli KT, Topçuoglu G (2015) Effectiveness of different irrigation procedures for removal of dentin debris from a simulated internal resorption cavity. Int J Artif Organs 38:165–169CrossRef Topçuoglu HS, Akti A, Düzgün S, Ceyhanli KT, Topçuoglu G (2015) Effectiveness of different irrigation procedures for removal of dentin debris from a simulated internal resorption cavity. Int J Artif Organs 38:165–169CrossRef
32.
Zurück zum Zitat Yamada RS, Armas A, Goldman M, Lin PS (1983) A scanning electron microscopic comparison of a high volume final flush with several irrigating solutions: part 3. J Endod 9:137–142CrossRef Yamada RS, Armas A, Goldman M, Lin PS (1983) A scanning electron microscopic comparison of a high volume final flush with several irrigating solutions: part 3. J Endod 9:137–142CrossRef
33.
Zurück zum Zitat Baker NA, Eleazer PD, Averbach RE, Seltzer S (1975) Scanning electron microscopic study of the efficacy of various irrigating solutions. J Endod 1:127–135CrossRef Baker NA, Eleazer PD, Averbach RE, Seltzer S (1975) Scanning electron microscopic study of the efficacy of various irrigating solutions. J Endod 1:127–135CrossRef
34.
Zurück zum Zitat Fornari VJ, Silva-Sousa YT, Vanni JR, Pécora JD, Versiani MA, Sousa-Neto MD (2010) Histological evaluation of the effectiveness of increased apical enlargement for cleaning the apical third of curved canals. Int Endod J 43:988–994CrossRef Fornari VJ, Silva-Sousa YT, Vanni JR, Pécora JD, Versiani MA, Sousa-Neto MD (2010) Histological evaluation of the effectiveness of increased apical enlargement for cleaning the apical third of curved canals. Int Endod J 43:988–994CrossRef
35.
Zurück zum Zitat Prado MC, Leal F, Simão RA, Gusman H, do Prado M (2017) The use of auxiliary devices during irrigation to increase the cleaning ability of a chelating agent. Restor Dent Endod 42:105–110CrossRef Prado MC, Leal F, Simão RA, Gusman H, do Prado M (2017) The use of auxiliary devices during irrigation to increase the cleaning ability of a chelating agent. Restor Dent Endod 42:105–110CrossRef
36.
Zurück zum Zitat De-Deus G, Marins J, Neves Ade A, Reis C, Fidel S, Versiani MA et al (2014) Assessing accumulated hard-tissue debris using micro-computed tomography and free software for image processing and analysis. J Endod 40:271–276CrossRef De-Deus G, Marins J, Neves Ade A, Reis C, Fidel S, Versiani MA et al (2014) Assessing accumulated hard-tissue debris using micro-computed tomography and free software for image processing and analysis. J Endod 40:271–276CrossRef
37.
Zurück zum Zitat Paqué F, Boessler C, Zehnder M (2011) Accumulated hard tissue debris levels in mesial roots of mandibular molars after sequential irrigation steps. Int Endod J 44:148–153CrossRef Paqué F, Boessler C, Zehnder M (2011) Accumulated hard tissue debris levels in mesial roots of mandibular molars after sequential irrigation steps. Int Endod J 44:148–153CrossRef
38.
Zurück zum Zitat Leoni GB, Versiani MA, Silva-Sousa YT, Bruniera JF, Pécora JD, Sousa-Neto MD (2017) Ex vivo evaluation of four final irrigation protocols on the removal of hard-tissue debris from the mesial root canal system of mandibular first molars. Int Endod J 50:398–406CrossRef Leoni GB, Versiani MA, Silva-Sousa YT, Bruniera JF, Pécora JD, Sousa-Neto MD (2017) Ex vivo evaluation of four final irrigation protocols on the removal of hard-tissue debris from the mesial root canal system of mandibular first molars. Int Endod J 50:398–406CrossRef
Metadaten
Titel
Micro-CT evaluation of different final irrigation protocols on the removal of hard-tissue debris from isthmus-containing mesial root of mandibular molars
verfasst von
Emmanuel João Nogueira Leal Silva
Carla Rodrigues Carvalho
Felipe Gonçalves Belladonna
Marina Carvalho Prado
Ricardo Tadeu Lopes
Gustavo De-Deus
Edson Jorge Lima Moreira
Publikationsdatum
10.05.2018
Verlag
Springer Berlin Heidelberg
Erschienen in
Clinical Oral Investigations / Ausgabe 2/2019
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-018-2483-1

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