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International Archives of Occupational and Environmental Health

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01.08.2015 | Original Article

Micronuclei and chromosome aberrations in subjects occupationally exposed to antineoplastic drugs: a multicentric approach

verfasst von: Massimo Moretti, Maria Giuseppa Grollino, Sofia Pavanello, Roberta Bonfiglioli, Milena Villarini, Massimo Appolloni, Mariella Carrieri, Laura Sabatini, Luca Dominici, Laura Stronati, Giuseppe Mastrangelo, Anna Barbieri, Cristina Fatigoni, Giovanni Battista Bartolucci, Elisabetta Ceretti, Francesca Mussi, Silvano Monarca

Erschienen in: International Archives of Occupational and Environmental Health | Ausgabe 6/2015

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Abstract

Objectives

Recently published works showed that occupational exposure to antineoplastic drugs (ANPD) is still frequent in hospital settings, despite significant safety policy improvements. The aim of this study was to assess the current level of occupational exposure to ANPD and any potentially associated cytogenetic damages in hospital nurses routinely handling ANPD.

Methods

Occupationally ANPD-exposed (n = 71) and ANPD-unexposed (n = 77; control) nurses were recruited on a voluntary basis from five hospitals in Northern and Central Italy. Evaluation of surface contamination and dermal exposure to ANPD was assessed by determining cyclophosphamide (CP) on selected surfaces (wipes) and on exposed nurses’ clothes (pads). The concentration of unmetabolized CP—as a biomarker of internal dose—was measured in end-shift urine samples. Biomonitoring of genotoxic effects (i.e., biological effect monitoring) was conducted by analyzing micronuclei (MN) and chromosome aberrations (CA) in peripheral blood lymphocytes. Genetic polymorphisms for enzymes involved in metabolic detoxification (i.e., glutathione S-transferases) were analyzed as well.

Results

We observed a significant increase in MN frequency (5.30 ± 2.99 and 3.29 ± 1.97; mean values ± standard deviation; p < 0.0001) in exposed nurses versus controls, as well as in CA detection (3.30 ± 2.05 and 1.84 ± 1.67; p < 0.0001), exposed subjects versus controls. Our results provide evidence that, despite safety controlled conditions, ANPD handling still represents a considerable genotoxic risk for occupationally exposed personnel.

Conclusions

Because both MN and CA have been described as being predictive of group-increased cancer risk, our findings point to a need for improving specific safety procedures in handling and administering ANPD.

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Titel: Micronuclei and chromosome aberrations in subjects occupationally exposed to antineoplastic drugs: a multicentric approach
verfasst von: Massimo Moretti
Maria Giuseppa Grollino
Sofia Pavanello
Roberta Bonfiglioli
Milena Villarini
Massimo Appolloni
Mariella Carrieri
Laura Sabatini
Luca Dominici
Laura Stronati
Giuseppe Mastrangelo
Anna Barbieri
Cristina Fatigoni
Giovanni Battista Bartolucci
Elisabetta Ceretti
Francesca Mussi
Silvano Monarca
Publikationsdatum: 01.08.2015
Verlag: Springer Berlin Heidelberg
Erschienen in: International Archives of Occupational and Environmental Health / Ausgabe 6/2015
Print ISSN: 0340-0131
Elektronische ISSN: 1432-1246
DOI: https://doi.org/10.1007/s00420-014-0993-y

Die Highlights vom Kongress des American College of Cardiology 2024

Springer Medizin

Micronuclei and chromosome aberrations in subjects occupationally exposed to antineoplastic drugs: a multicentric approach

Abstract

Objectives

Methods

Results

Conclusions

Die Highlights vom Kongress des American College of Cardiology 2024

Springer Medizin

Abstract

Objectives

Methods

Results

Conclusions

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