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28.10.2017 | Original Article

MicroRNA Expression in Laser Micro-dissected Breast Cancer Tissue Samples – a Pilot Study

verfasst von: Edward Seclaman, Diana Narita, Andrei Anghel, Natalia Cireap, Razvan Ilina, Ioan Ovidiu Sirbu, Catalin Marian

Erschienen in: Pathology & Oncology Research | Ausgabe 1/2019

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Abstract

Breast cancer continues to represent a significant public health burden despite outstanding research advances regarding the molecular mechanisms of cancer biology, biomarkers for diagnostics and prognostic and therapeutic management of this disease. The studies of micro RNAs in breast cancer have underlined their potential as biomarkers and therapeutic targets; however most of these studies are still done on largely heterogeneous whole breast tissue samples. In this pilot study we have investigated the expression of four micro RNAs (miR-21, 145, 155, 92) known to be involved in breast cancer, in homogenous cell populations collected by laser capture microdissection from breast tissue section slides. Micro RNA expression was assessed by real time PCR, and associations with clinical and pathological characteristics were also explored. Our results have confirmed previous associations of miR-21 expression with poor prognosis characteristics of breast cancers such as high stage, large and highly proliferative tumors. No statistically significant associations were found with the other micro RNAs investigated, possibly due to the small sample size of our study. Our results also suggest that miR-484 could be a suitable endogenous control for data normalization in breast tissues, these results needing further confirmation by future studies. In summary, our pilot study showed the feasibility of detecting micro RNAs expression in homogenous laser captured microdissected invasive breast cancer samples, and confirmed some of the previously reported associations with poor prognostic characteristics of breast tumors.

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Titel: MicroRNA Expression in Laser Micro-dissected Breast Cancer Tissue Samples – a Pilot Study
verfasst von: Edward Seclaman
Diana Narita
Andrei Anghel
Natalia Cireap
Razvan Ilina
Ioan Ovidiu Sirbu
Catalin Marian
Publikationsdatum: 28.10.2017
Verlag: Springer Netherlands
Erschienen in: Pathology & Oncology Research / Ausgabe 1/2019
Print ISSN: 1219-4956
Elektronische ISSN: 1532-2807
DOI: https://doi.org/10.1007/s12253-017-0343-y

Springer Medizin

MicroRNA Expression in Laser Micro-dissected Breast Cancer Tissue Samples – a Pilot Study

Abstract

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