Background
Methods
Patient’s characteristics
Sensitive | Resistant | |
---|---|---|
Cases number (N) | 24 | 21 |
Age (range, years) | 52(41–67) | 52(32–68) |
FIGO Stage | ||
I(N) | 3 | 0 |
II(N) | 1 | 2 |
III(N) | 18 | 17 |
IV(N) | 2 | 2 |
Tumor Grade | ||
1(N) | 1 | 1 |
2(N) | 7 | 2 |
3(N) | 16 | 18 |
Recurrence | ||
No (N) | 2 | 0 |
Yes (N) | 22 | 21 |
PFS (Range,months) | 22(12–78) | 6(3–11) |
OS (Range, months) | 42.5(18–144) | 17(7–65) |
Survival | ||
No (N) | 12 | 20 |
Yes (N) | 12 | 1 |
Cell culture and transfection
RNA extraction and microRNA microarray
Realtime RT-PCR validation
Western blotting
Dual luciferase reporter assay
Primer | Sequence 5′-3′ |
---|---|
RAB34-WT-F | CCG CTCGAGGGGCTGAGGAGACTGTTC |
RAB34-WT-R | GAATGCGGCCGCGCTCGTAACAAAGAAATTTTAATGCATAAG |
RAB34-Mut-F | TTATCCAGTCGATACTGCTGCCTCTTGGTGGCAGTA |
RAB34-Mut-R | CAGCAGTATCGACTGGATAAAGTCAGTGCAAATGT |