Background
Liver cancer is reportedly the 6th most common cancer and the 4th most lethal cause of cancer-related death worldwide, with hepatocellular carcinoma (HCC) accounting for ~ 80% of these cases [
1]. Due to various pathogeneses involved in HCC tumor progression, the heterogeneity of HCC cells, and the late stage during which HCC is usually identified, the prognosis of HCC patients is poor and, despite the recent discovery of subclinical molecular targets, the overall survival rate of HCC remains low [
2]. Therefore, it is necessary to continue investigating the molecular mechanisms associated with HCC progression in order to identify additional prognostic indicators.
MicroRNAs (miRNAs) are small, noncoding RNAs that participate in the posttranscriptional regulation of gene expression [
3], and accumulating data indicate that miRNAs play an important role in modulating multiple biological behaviors associated with cancer [
4]. Moreover, miRNA levels are correlated with neoplasm staging, tumor relapse, drug tolerance, and prognosis [
5‐
7]. However, the mechanisms underlying the miRNA-specific regulation of tumor progression have not been completely investigated.
Aerobic organisms exhibit sophisticated antioxidation mechanisms in order to maintain a reducing environment in cells. Thioredoxin-1 (TRX1) is a key contributor to intracellular redox homeostasis and promotes energy and carbohydrate metabolism [
8]. Mechanistically, TRX1 responds to reactive oxygen species produced by cellular respiration and metabolism and facilitates the reduction of other proteins at the cost of its own oxidation [
9]. Thioredoxin reductase-1 (TXNRD1) is uniquely capable of utilizing electrons from NADPH to recover the reduced state of TRX1. Interestingly, TXNRD1 is upregulated in many human malignancies and promotes cancer progression [
10], and attenuation of TXNRD1 levels effectively suppresses the growth of tumor cells [
11].
In this study, we investigated the regulatory relationship between miR-125b-5p and TXNRD1 in HCC cells. Furthermore, we examined the effect of miR-125b-5p on the biological function of HCC cell lines.
Methods
Clinical samples
A total of 13 pairs of fresh HCC and matched adjacent tissues were acquired from patients undergoing their first hepatectomy and diagnosed with HCC between May 2016 and June 2018 at the Zhuhai People’s Hospital, Jinan University (Zhuhai, China). None of the patients had received radiotherapy or chemotherapy prior to the hepatectomy. Ethics approval for this study was obtained from the Zhuhai People’s Hospital Institutional Review Board with approval number [2016 trial (the institute) in subclause (10)].
Cell culture
Two human HCC cell lines (Huh7 and SK-Hep-1) were obtained from the Cell Bank of Type Culture Collection (Chinese Academy of Sciences, Shanghai, China). Cells were cultured with Dulbecco’s modified Eagle’s medium (Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS; Invitrogen) and incubated at 37 °C with 5% CO2.
Gene- and miRNA-expression datasets
Data regarding miR-125b-5p levels from 372 HCC patients (follow-up information was included for 328 patients), and data regarding TXNRD1 levels from 351 HCC patients (concurrent data concerning miR-125b-5p levels were included for 347 patients) were obtained from The Cancer Genome Atlas (TCGA;
https://tcga-data.nci.nih.gov/tcga/). Additionally, five microarray datasets (GSE22058, GSE54751, GSE21362, GSE12717, and GSE10694) were obtained from the Gene Expression Omnibus (GEO;
http://www.ncbi.nlm.nih.gov/geo).
Gene set enrichment analysis (GSEA)
GSEA was performed to identify gene sets or pathways associated with miR-125b-5p levels in the TCGA dataset. The 347 samples, for which miRNA- and mRNA-expression profiles were available, were used for GSEA after being divided into two groups: miR-125b-5p high or low expression. The gene pool, including the RAMASWAMY metastasis-associated gene set (
http://software.broadinstitute.org/gsea/msigdb/cards/RAMASWAMY_METASTASIS_UP), was a gift from Zhongshan University (Guangzhou, China). We used GSEA software (v.2.0;
http://www.broadinstitute.org/gsea), with
P < 0.05 considered statistically significant.
miRNA transfection
miR-125b-5p mimic/inhibitor was purchased from RiboBio (Guangzhou, China). A miR mimic/inhibitor was transfected into HCC cells upon their reaching 20% to 30% confluence and using Lipofectamine RNAiMAX (Invitrogen) at a working concentration of 100 nM according to manufacturer instructions. Cells were used for analysis after a 24- to 72-h culture.
Plasmid transfection
Huh7, SK-Hep-1, and HEK293T cells were placed in a plate (Nest Biotechnology, Wuxi, China) and transfected with plasmids upon reaching 80% to 90% confluence using Lipofectamine 3000 (Invitrogen) according to manufacturer instructions, after with cells were used for analyses after 48 h. The TXNRD1-expression plasmid and those harboring either wild-type (WT) or mutant versions of the TXNRD1 3′ untranslated region (UTR) were obtained from ViGene Biosciences (Shandong, China).
Luciferase-reporter assay
The fragment of the TXNRD1 3′ UTR containing the miR-125b-5p binding site (named WT) and fragment containing the site modified by site-directed mutagenesis (named Mutant) were cloned into the psiCHECK-2.0 vector (Promega, Madison, WI, USA). For the 3′ UTR dual-luciferase reporter assay, miR-125b-5p-overexpressing or -silenced HEK293 cells were co-transfected with WT or Mutant plasmids. Luciferase activity was detected using the luciferase reporter assay kit (Promega) at 72-h post-transfection.
Quantitative reverse transcription polymerase chain reaction (qRT-PCR)
RNA was acquired using an RNA extraction kit (Invitrogen) according to manufacturer instructions, and qRT-PCR was performed using a SYBR Green PCR kit (Takara, Dalian, China), with
glyceraldehyde 3-
phosphate dehydrogenase and U6 used as internal controls for protein-coding genes and miR-125b-5p, individually. Primer specificity was verified prior to use, and assays were performed in triplicate. Primer sequences of primer are listed in Additional file
1: Table S1.
Western blotting
HCC cells were lysed on ice using radioimmunoprecipitation assay buffer (Beyotime, Nanjing, China) containing phosphatase and protease inhibitors (Roche, Basel, Switzerland). Total protein was quantitated using the Bradford assay (Thermo Fisher Scientific, Waltham, MA, USA). Equivalent protein lysates were subjected to 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred onto a polyvinylidene fluoride membrane that was blocked using 5% nonfat milk for 40 min at 37 °C, followed by incubation at 4 °C for > 8 h with antibodies against TXNRD1 (Proteintech, Rosemont, IL, USA) and horseradish peroxidase-conjugated secondary antibodies (Cell Signaling Technology, Danvers, MA, USA). Peroxidase activity was visualized using an enhanced chemiluminescence substrate (Bio-Rad Laboratories, Hercules, CA, USA).
Cell Counting Kit (CCK)-8 assay
Cells were placed in 96-well plates (~ 800 cells/well; Nest Biotechnology, Jiangsu, China), and 10 μL of CCK-8 solution (Dojindo Laboratories, Osaka, Japan) was added to each well at 24-, 48-, 72-, 96-, or 120-h post-treatment. After 2-h incubation in the dark, absorbance was detected at 450 nm.
5-ethynyl-2′-deoxyuridine (EdU)-incorporation assay
Cells in 96-well plates at a density of 5000 cells/well were analyzed utilizing a Cell-Light EdU Apollo 567 kit (RiboBio) according to the manufacturer’s instructions. We used an inverted fluorescence microscope (Olympus, Tokyo, Japan) to count cells in five random fields, with experiments performed in triplicate using independent samples.
Transwell-migration and Boyden-invasion assays
For the Transwell-migration assay, ~ 1 × 105 cells suspended in 100 µL of serum-free medium were placed in the upper layer of the Transwell (Corning Life Sciences, Lowell, MA, USA) while the lower layer received 500 µL of medium containing 10% FBS. We washed the inserts three times using phosphate-buffered saline, and fixed cells that adhered to the Transwell were incubated with methanol for ~ 10 min at room temperature, followed by dying with crystal violet for 20 min. Experiments were performed in triplicate. Boyden-invasion assay were performed using the same protocol as that for the cell-migration assay, but the membrane used was coated with 24 mg/mL Matrigel (BD Biosciences, Franklin Lakes, NJ, USA).
Statistical analysis
SPSS software (v.16.0; SPSS Inc., Chicago, IL, USA) was used for data analysis. Data represent the mean ± standard error of the mean of at least three replicates. Student’s
t test was used to evaluate differences between two groups, and one-way analysis of variance (ANOVA), followed by Dunnett’s multiple comparison test was used to assess differences between multiple groups. The results of the CCK-8 assay were evaluated using multi-way classification ANOVA, and overall and relapse-free survival rates were analyzed using the Kaplan–Meier method [
12]. Correlations were analyzed with Pearson’s correlation. A
P < 0.05 was considered statistically significant.
Discussion
In this study, we found that miR-125b-5p expression was attenuated in HCC tissues according to the analysis of five GEO datasets and a TCGA cohort, suggesting its possible involvement in regulating HCC progression. Although one study indicated its role in tumor progression [
13], the overwhelming majority of reports suggest that miR-125b-5p as a tumor suppressor inhibits multiple biological functions in a variety of tumors, including those associated with HCC [
14‐
19]. Additionally, previous studies report that miR-125b-5p suppresses HCC proliferation and metastasis by regulating the expression of downstream signaling molecules [
17‐
22]. The findings of present study confirmed, for the first time, that miR-125b-5p targets
TXNRD1 to inhibit HCC cell proliferation, migration, and invasion by downregulating TXNRD1 expression.
To identify potential miR-125b-5p targets in HCC cells, we screened predicted targets forecasted as upregulated in HCC tissues according to a TCGA cohort and reported as cancer-promoting genes in HCC in the literature. qRT-PCR analysis and co-transfection of HCC cells with a miR-125b-5p mimic or inhibitor revealed TXNRD1 as the target. Previous studies identified TXNRD1 as a regulator of endocellular oxidative stress and a promoter of tumor development [
23,
24], with irreversible suppression of cytoplasmic TXNRD1 levels demonstrated as a novel direction for cancer treatment [
11,
25,
26]. Additionally, studies reported that TXNRD1 levels are upregulated in HCC and negatively correlated with HCC-patient prognosis [
27,
28]. In the present study, our data demonstrated that TXNRD1 promoted the proliferation, migration, and invasion of HCC cells, which provided insight into the previously unknown molecular mechanisms associated with TXNRD1 in HCC. Metabolic analysis showed that TXNRD1 is crucial for the final step in the synthesis of nucleic acids by providing reductive equivalents to ribonucleotide reductase [
29], and failure to maintain sufficient stores of 2′-deoxyribonucleotides resulted in damaged DNA and cell cycle arrest in
TXNRD1-deficient T cells [
30]. These findings and our results from the present study suggest that the mechanisms underlying TXNRD1 regulation of HCC status might be associated with oxidative stress or nucleotide biosynthesis; however, further studies are required to clarify these roles.
Here, we demonstrated the tumor-suppressive property of miR-125b-5p via its targeting of TXNRD1, which broadens our understanding of the molecular mechanism associated with miR-125b-5p-related functions in HCC cells. Nevertheless, the mechanism underlying the attenuated expression of miR-125b-5p in HCC cells remains to be investigated in future research.
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