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Journal of Cancer Research and Clinical Oncology

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01.04.2011 | Original Paper

Molecular and histological evaluation of tumor necrosis factor-alpha expression in Helicobacter pylori-mediated gastric carcinogenesis

verfasst von: Cinghu Senthilkumar, Sivasithambaram Niranjali, Venkatraman Jayanthi, Thiyagarajan Ramesh, Halagowder Devaraj

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 4/2011

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Abstract

Purpose

Helicobacter pylori (H. pylori) is considered to be a major factor contributing to gastric mucosal damage by stimulating mucosal macrophage production of inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), but the inflammatory responses within the gastric mucosa in vivo are not well known. Therefore, this study was designed to investigate the expression of TNF-α induced by H. pylori infection which is involved in the tumor initiation and promotion of gastric carcinogenesis.

Methods

This study was carried out in 200 patients, consisting of normal gastric mucosa (n = 20), mucosa with chronic gastritis (n = 63), intestinal metaplasia (n = 20), dysplasia (n = 11), and gastric adenocarcinoma (n = 86), in which the H. pylori status has been analyzed. The expression of TNF-α was studied at mRNA as well as protein level using RT-PCR and western blotting, respectively. The localization of TNF-α was also studied semiquantitatively by immunohistochemistry.

Results

The RT-PCR and western blotting results of TNF-α mRNA and protein expressions were significantly increased in chronic gastritis, intestinal metaplasia, dysplasia and gastric adenocarcinoma patients, respectively. Immunohistochemical study also showed the increased expression of TNF-α in the similar way.

Conclusion

Over expression of TNF-α showed a significant severity-dose–response as risk markers from preneoplastic lesions to gastric cancer.

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Titel: Molecular and histological evaluation of tumor necrosis factor-alpha expression in Helicobacter pylori-mediated gastric carcinogenesis
verfasst von: Cinghu Senthilkumar
Sivasithambaram Niranjali
Venkatraman Jayanthi
Thiyagarajan Ramesh
Halagowder Devaraj
Publikationsdatum: 01.04.2011
Verlag: Springer-Verlag
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 4/2011
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-010-0921-9

Springer Medizin

Molecular and histological evaluation of tumor necrosis factor-alpha expression in Helicobacter pylori-mediated gastric carcinogenesis