Multiple-Dose Pharmacokinetics of Ozanimod and its Major Active Metabolites and the Pharmacodynamic and Pharmacokinetic Interactions with Pseudoephedrine, a Sympathomimetic Agent, in Healthy Subjects
verfasst von:
Jonathan Q. Tran, Peijin Zhang, Susan Walker, Atalanta Ghosh, Mary Syto, Xiaomin Wang, Sarah Harris, Maria Palmisano
The aims of this study were to characterize the multiple-dose pharmacokinetics (PK) of ozanimod’s major active metabolites (CC112273 and CC1084037) and to evaluate the pharmacodynamic and PK interactions with pseudoephedrine (PSE).
Methods
In this phase 1, single-center, randomized, double-blind, placebo-controlled study, 56 healthy adult subjects were randomized to receive either placebo or ozanimod once daily for 30 days (0.23 mg on days 1–4, 0.46 mg on days 5–7, 0.92 mg on days 8–10, and 1.84 mg on days 11–30). On day 30, a single oral dose of PSE 60 mg was co-administered with placebo or ozanimod. Maximum time-matched change in systolic blood pressure (SBP) from baseline (day 29) following PSE administration on day 30 was calculated. Plasma PK parameters for ozanimod, CC112273, CC1084037, and PSE were estimated using noncompartmental methods.
Results
Fifty-two subjects (92.9%) completed the study. Following multiple dosing, approximately 94% of circulating total active drug exposure was represented by ozanimod (6%), CC112273 (73%), and CC1084037 (15%). Exposures of CC112273 and CC1084037 were highly correlated. Mean maximum time-matched change from baseline for SBP was not significantly different between ozanimod + PSE and placebo + PSE. Ozanimod also had no effect on the PK of PSE. Co-administration of ozanimod with a single dose of PSE in healthy subjects was generally well tolerated. While CC112273 and CC1084037 selectively inhibited monoamine oxidase (MAO)-B in vitro, both active metabolites do not inhibit platelet MAO-B activity in vivo.
Conclusion
Concomitant administration of ozanimod with PSE, a sympathomimetic agent, did not potentiate the effects on blood pressure.
In vitro data showed that ozanimod’s major active metabolites, CC112273 and CC1084037, selectively inhibited monoamine oxidase (MAO)-B. MAO inhibitors, when taken in combination with sympathomimetic amines, can cause sudden blood pressure elevations.
We conducted a phase 1 study in healthy subjects to (1) characterize the multiple-dose pharmacokinetic (PK) properties of CC112273 and CC1084037, (2) evaluate the PK and pharmacodynamic interactions with pseudoephedrine, a sympathomimetic agent, and (3) evaluate the effect of CC112273 and CC1084037 on human platelet MAO-B activity.
What was learned from this study?
Following multiple dosing, approximately 94% of circulating total active drug exposure was represented by ozanimod (6%), CC112273 (73%), and CC1084037 (15%).
While CC112273 and CC1084037 selectively inhibited MAO-B in vitro, both active metabolites did not inhibit platelet MAO-B activity in vivo. Concomitant administration of ozanimod with pseudoephedrine did not result in PK or pharmacodynamic interactions.
Digital Features
This article is published with digital features to facilitate understanding of the article. You can access the digital features on the article’s associated Figshare page. To view digital features for this article go to https://doi.org/10.6084/m9.figshare.12918965.
Anzeige
Introduction
Ozanimod, a sphingosine 1-phosphate (S1P) receptor modulator that binds with high affinity selectively to S1P receptors 1 (S1P1) and 5 (S1P5), is approved in the United States for the treatment of adults with relapsing forms of multiple sclerosis (MS) and in the European Union for the treatment of adults with relapsing-remitting MS. Ozanimod blocks the capacity of lymphocytes to egress from lymph nodes, reducing the number of lymphocytes in peripheral blood [1]. The mechanism by which ozanimod exerts therapeutic effects in MS is unknown but may involve the reduction of lymphocyte migration into the central nervous system. Ozanimod is also in clinical development for the treatment of moderate to severe ulcerative colitis and Crohn’s disease [2, 3].
Ozanimod is extensively metabolized to form a number of circulating active metabolites, including two major and interconverting active metabolites CC112273 and CC1084037 with similar activity and selectivity for S1P1 and S1P5 [4]. Following oral administration of ozanimod single doses of 0.46 mg and 0.92 mg in 56 healthy subjects, ozanimod absorption was slow, with a median time to reach maximum observed concentration (Tmax) of 8 h. Median Tmax values for CC112273 and CC1084037 were approximately 10 and 16 h, respectively. Dose-proportional increases in exposures were observed for ozanimod, CC112273, and CC1084037. Exposures of CC112273 and CC1084037 also were highly correlated. The mean terminal elimination half-life (t1/2) for ozanimod was approximately 20–22 h while the mean t1/2 values for CC112273 and CC1084037 were similar at approximately 10 days [4].
In vitro data showed that CC112273 and CC1084037 selectively inhibited monoamine oxidase (MAO)-B over MAO-A. The half maximal inhibitory concentration (IC50) values for MAO-B are 5.72 nM and 58 nM for CC112273 and CC1084037, respectively (data on file). The IC50 values for MAO-A are greater than 10,000 nM for both major active metabolites. Two isoenzymes of MAO (MAO-A and MAO-B) are present in most mammalian tissues, but the proportions of the two isoenzymes vary from tissue to tissue [5, 6]. Moreover, some human tissues express only one form of MAO (e.g., fibroblasts and placenta express only MAO-A whereas platelets and lymphocytes express only MAO-B) [7]. MAO catalyzes the oxidative deamination of various monoamines, such as histamine, 5-hydroxytryptamine, and the catecholamines (e.g., dopamine, epinephrine, and norepinephrine) to inactive derivatives [8].
Monoamine oxidase inhibition can lead to peripheral or central neurotransmitter accumulation. Monoamine oxidase inhibitors, when taken in combination with sympathomimetic amines, can cause sudden blood pressure (BP) elevations that may lead to hypertensive crises [9‐11]. Pseudoephedrine (PSE), a sympathomimetic agent, is found in many over-the-counter medicines to relieve nasal congestion. The sympathomimetic actions of PSE are primarily the result of norepinephrine release [12]. Inhibition of MAO may reduce the deamination of norepinephrine, leading to pressor effect enhancement of PSE.
Anzeige
This study was conducted to characterize the multiple-dose pharmacokinetic (PK) properties of ozanimod’s major active metabolites and to evaluate the effect of ozanimod on the pressor response to a single-dose administration of PSE and on the PK of PSE in healthy adult subjects. The study also evaluated the effect of CC112273 and CC1084037 on human platelet MAO-B activity.
Methods
Ethics
The study protocol and informed consent were reviewed and approved by an institutional review board (IntegReview, Austin, TX, USA; closure date 22 January 2019). All subjects provided written informed consent before study entry. The study was conducted in accordance with the ethical principles of Good Clinical Practice and the Declaration of Helsinki.
Study Population
Healthy female or male subjects (N = 56) aged 18–55 years, with a body weight of at least 50 kg and body mass index (BMI) of 18.0–30.0 kg/m2 were included in the study. Subjects were excluded from the study if they had a seated systolic BP (SBP) outside 90–140 mmHg, seated diastolic BP (DBP) 50–90 mmHg, or seated pulse rate outside 55–90 bpm; a history of syncope associated with hypotension or a history of orthostatic hypotension; a positive drug/alcohol test; a history of alcoholism, drug abuse, or addiction within 24 months; or a history of any abnormality or illness that, in the opinion of the investigator, may affect absorption, distribution, metabolism, or elimination of the study drugs or would limit the subject’s ability to participate in and complete the study. Subjects were prohibited from using any systemic over-the-counter medication (excluding acetaminophen up to 1 g/day), dietary or herbal supplements within 7 days (28 days for St. John’s wort) prior to the first dose of study drug, and any systemic prescription medication (excluding hormonal contraceptives) within 28 days (90 days for MAO inhibitors) or five times the elimination half-life, whichever is longer, prior to the first dose of study drug and during the study.
Study Design
This was a phase 1, single-center, randomized, double-blind, placebo-controlled study in healthy adult subjects. Subjects were screened for participation within 28 days prior to the first dose of study drug. All eligible subjects were admitted to the clinical research unit (CRU) on day −2 and were domiciled in the CRU until day 31. Eligible subjects were enrolled and randomized 1:1 within either the male or female strata to ozanimod or placebo. Subjects received either placebo or ozanimod once daily (QD) for 30 days (0.23 mg [equivalent to ozanimod HCl 0.25 mg] QD on days 1–4, 0.46 mg [equivalent to ozanimod HCl 0.5 mg] QD on days 5–7, 0.92 mg [equivalent to ozanimod HCl 1 mg] QD on days 8–10, and 1.84 mg [equivalent to ozanimod HCl 2 mg] QD on days 11–30). On day 30, a single oral dose of PSE 60 mg was co-administered with placebo or ozanimod. On days 29 and 30, study drug was administered after an overnight fast of at least 10 h and subjects remained fasted until at least 4 h after dosing. Meals and mealtimes on days 29 and 30 were also standardized. Following discharge from the CRU on day 31, follow-up safety assessments were conducted at 7 ± 2 and 75 ± 10 days after the last dose.
PK Blood Sampling Schedule and Bioanalysis
PK blood samples for ozanimod and its active metabolites were collected at predose and 1, 2, 4, 6, 8, 10, 12, 14, 16, and 24 h postdose on days 1, 5, 8, and 28. Trough PK blood samples were collected at predose on days 26, 27, and 30. PK blood samples for PSE were collected at predose and 1, 2, 4, 6, 8, 10, 12, and 24 h postdose on day 30.
Plasma concentrations of ozanimod, major active metabolites (CC112273 and CC1084037), and minor active metabolites (RP101988, RP101442, and RP112289) were determined using the validated liquid chromatography–tandem mass spectrometry method (ICON, Whitesboro, NY). To measure plasma concentrations, ozanimod and its metabolites were extracted from a 400-μL aliquot of human K2 ethylenediaminetetraacetic acid plasma by support–liquid extraction and then analyzed using reversed-phase high-performance liquid chromatography with electrospray MS/MS detection. The calibrations were accomplished by weighted linear regressions (1/x2) of the ratio of the peak area of the analyte to that of the added deuterated internal standards and plotting to the corresponding nominal concentrations of PSE. The methods were validated over the concentration ranges of 4–2000 pg/mL for both ozanimod and CC1084037, and 25–10,000 pg/mL for CC112273. In addition, the assays were validated for the minor metabolites with the concentration ranges of 16–4000 pg/mL for RP101988, 8–4000 pg/mL for RP101442, and 4–1600 pg/mL for RP112289. The interassay precision values for ozanimod, CC112273, CC1084037, RP101988, RP101442, and RP112289 were at most 9.8%. The accuracy values for ozanimod and its metabolites were − 6.7% to 4.2%.
Plasma concentrations of PSE were determined by using the validated high-performance liquid chromatography–mass spectrometry method (PRA, Lenexa, KS). To measure plasma concentrations, a 50-μL aliquot of human dipotassium ethylenediaminetetraacetic acid plasma by protein precipitation was analyzed using reversed-phase high-performance liquid chromatography with electrospray MS/MS detection. The calibrations were accomplished by weighted linear regressions (1/x2) of the ratio of the peak area of the analyte to that of the added deuterated internal standards and plotted to the corresponding nominal concentrations of ozanimod and its metabolites, respectively. The methods were validated over the concentration ranges of 0.5–500 ng/mL for PSE. The interassay precision values (%CV) for PSE were at most 12.3%. The accuracy (%bias) values for PSE were within the range of − 3.3% to 2.8%.
PD Blood Sampling Schedule and Bioanalysis
Blood samples for the measurement of MAO-B activity in platelets were collected at screening, predose on days 1, 5, 8, 26 to 30, and 12 h postdose on day 1. MAO-B activity in human platelets was measured using the OxiSelect™ Monoamine Oxidase Assay kit (a simple and sensitive quantitative fluorometric assay for measuring amine oxidase activity in biological samples) and a Biotek Synergy 2 plate reader. Briefly, the assay employs quantitative fluorometric measurement of amine oxidase activity in biological samples. The kit is based on a reaction between MAO-B and its substrate benzylamine that generates H2O2. Benzylamine is a substrate for MAO-B and semicarbizide-sensitive amine oxidases. The use of MAO-B inhibitor Pargyline distinguishes activity between these enzymes. In the presence of horseradish peroxidase, the fluorometric probe and H2O2 react to produce resorufin, which in turn is quantified by fluorescence. Activity of MAO-B is then calculated and reported in units per liter. The assay was qualified and samples tested by Covance Translational Biomarker Solutions. MAO-B activity ranged from 0.016 to 0.766 units/L. Of the 581 samples received, 577 were within the limits of quantitation of 0.014 to 1.778 units/L.
Anzeige
Cardiovascular, PK, and Safety Assessments
On days 29 and 30, SBP and DBP were measured in triplicate after subjects were resting for at least 10 min in a supine position at 0 (predose) and 1, 2, 3, 4, 5, 6, 7, 8, 10, 12, and 24 h after dosing. Each of the triplicate measurements was separated by approximately 5 min, and all triplicate measurements were completed within approximately 15 min. The 0-h triplicate measurements on day 29 were collected within approximately 15 min before dosing. The 24-h postdose triplicate measurements on day 29 were collected within approximately 15 min before dosing on day 30, and these measurements were also used as day 30, 0-h measurements. BP at each nominal time point was calculated as a mean of the triplicate measurements.
Pharmacokinetic parameters were calculated using noncompartmental analysis (Phoenix™ WinNonlin®, version 8.0.0.3176) and actual sample collection time. The following PK parameters were estimated for ozanimod and its active metabolites (CC112273, CC1084037, RP101988, RP101442, and RP112289) and PSE: maximum observed concentration (Cmax), Tmax, and area under the concentration–time curve from time 0–24 h (AUC0–24). Total active AUC0–24 for ozanimod was determined as the sum of ozanimod and all active metabolites AUC0–24, corrected for molecular weight. Plasma concentration values that were below the limits of quantitation (BLQ) were replaced with zero for the mean plasma concentration–time profiles. For PK parameter calculation, BLQ values prior to the first quantifiable value were replaced with zero, and BLQ values after the first quantifiable value were treated as missing.
Physical examinations, 12-lead electrocardiograms (ECGs), vital sign measurements, and clinical laboratory tests were performed. Adverse events (AEs) and concomitant medications were monitored throughout the study to assess safety.
Statistical Evaluation
No formal hypothesis testing was planned. Using a precision approach, a sample size of 25 each for ozanimod and placebo was expected to produce a two-sided 90% confidence interval (CI) with a distance from the difference in means to the limits that is equal to 6.64 when the estimated standard deviation (SD) is 14 mmHg. Because of potential subject dropout, up to 30 subjects were planned for each treatment group to ensure 25 evaluable subjects within each group.
Anzeige
The primary cardiovascular endpoint was the maximum time-matched change in SBP from baseline (day 29) following PSE administration on day 30. To calculate the time-matched changes for each subject, day 30 value was subtracted from day 29 value at the corresponding nominal time. The maximum of these time-matched changes was computed for each subject. A statistical analysis was performed to compare ozanimod + PSE with placebo + PSE using a linear model with treatment group and sex as fixed effects. The least squares mean (LSM) difference between the two treatment groups and associated 90% CI were provided.
To estimate the effect of ozanimod on PSE exposure, an analysis of variance was performed for the PSE primary PK parameters (Cmax and AUC0–24). The primary PK parameters were log-transformed and evaluated in a mixed-effect model with treatment group and sex as fixed effects and subjects as a random effect. Logarithmic differences in LSM of primary PK parameters from PSE co-administered with ozanimod (as test treatment) to PSE co-administered with placebo (as reference treatment) were constructed. Upon back transformation, the PSE geometric LSM ratios of ozanimod over the placebo group and associated 90% CI were calculated.
The relationship between CC112273 concentrations and change from baseline in platelet MAO-B activity was plotted. Similar analysis was performed for CC1084037 concentrations.
The incidence, severity, and relationship of AEs and clinically significant changes from baseline in clinical laboratory tests, vital signs, and ECGs were summarized using descriptive statistics by treatment group. All statistical tabulations and analyses were performed using SAS, version 9.3 (SAS Institute, Cary, NC).
Anzeige
Results
Subject Disposition and Baseline Characteristics
Fifty-six subjects were randomized and 52 subjects (92.9%) completed the study. All subjects in the ozanimod group completed the study. Four subjects (7.1%) in the placebo group did not complete the study: two subjects withdrew from the study and two subjects were discontinued from the study because of an AE. Thirty-one (57.4%) subjects were male and 23 (42.6%) were female. The mean (SD) age and BMI were 37.5 (11.34) years and 26.11 (2.583) kg/m2, respectively. Overall, demographics and baseline characteristics were generally similar between the two treatment groups.
Multiple-Dose PK of Ozanimod and Major Active Metabolites
The mean (SD) plasma concentration–time profiles for ozanimod and its major active metabolites following dosing initiation on day 1 (0.23 mg), dose escalation on day 5 (0.46 mg), and on day 28 (1.84 mg) are shown in Fig. 1. Following dosing initiation, the formation of the active metabolites CC112273 and CC1084037 occurred approximately 2 and 4 h after dosing. Systemic exposure of CC112273 was the highest followed by ozanimod and CC1084037. After 28 days of dosing, systemic exposure of CC112273 remains the highest followed by CC1084037 and then ozanimod. Plasma PK parameters for ozanimod, CC112273, and CC1084037, following dosing initiation on day 1 (0.23 mg), dose escalation on day 5 (0.46 mg), and after 28 days of repeated dosing (1.84 mg) are summarized in Table 1. Median Tmax was approximately 8–10 h across dosing days for ozanimod, approximately 10–12 h across dosing days (approximately 2 h after ozanimod) for CC112273, and was highly variable (4–24 h) for CC1084037 because of flat PK profiles during the 24-h dosing interval. Upon ozanimod 0.23 mg initiation (day 1), the AUC0–24 values of CC112273 and CC1084037 were approximately twofold and 0.2-fold that of the parent AUC0–24, respectively. Ozanimod, CC112273, and CC1084037 each represented approximately 28%, 53%, and 5%, respectively, of circulating total active drug exposure. The metabolite/parent (M/P) ratios and percentage of total active AUC0–24 for CC112273 and CC1084037 continued to increase with repeated dosing. On day 28, the mean M/P ratios of the AUC0–24 for CC112273 and CC1084037 were approximately 13-fold and 2.6-fold, respectively. Ozanimod, CC112273, and CC1084037 each represented approximately 6%, 73%, and 15%, respectively, of circulating total active drug exposure. Together, ozanimod, CC112273, and CC1084037 contributed to approximately 94% of circulating total active drug exposure after multiple dosing. The other minor active metabolites (RP101988, RP101442, and RP112289) together contributed to the remaining 6% of circulating total active drug exposure (data not shown).
Fig. 1
Mean (SD) plasma concentration–time profiles for ozanimod, CC112273, and CC1084037 over the 24-h dosing interval following ozanimod dosing initiation on day 1 (0.23 mg; a), dose escalation on day 5 (0.46 mg; b), and after 28 days of repeated dosing (1.84 mg, c); N = 28. SD standard deviation
Table 1
Summary statistics of pharmacokinetic parameters for ozanimod and its major metabolites following dosing initiation on day 1 (0.23 mg), dose escalation on day 5 (0.46 mg), and after 28 days of repeated dosing (1.84 mg); N = 28
PK parameter (unit)
Ozanimod 0.23 mg (day 1)
Ozanimod 0.46 mg (day 5)
Ozanimod 1.84 mg (day 28)
Ozanimod
CC112273
CC1084037
Ozanimod
CC112273
CC1084037
Ozanimod
CC112273
CC1084037
Cmax
(pg/mL)
42.6
(10.9)
72.9
(17.3)
9.66
(3.36)
114
(33.4)
344
(89.4)
53.7
(15.1)
639
(213)
6338
(1913)
1250
(354)
Tmax
(h)
10.1
(6.15, 14.1)
12.1
(8.05, 24.0)
24.0
(10.1, 24.0)
8.19
(4.07, 14.1)
10.1
(6.13, 24.0)
20.0
(4.05, 24.0)
8.07
(6.13, 12.1)
10.1
(0.00, 12.1)
4.06
(0.00, 24.0)
AUC0–24
(pg × h/mL)
684
(171)
1160
(304)
117
(45.6)
2039
(644)
6775
(1852)
1080
(293)
11,370
(4009)
125,970
(41,328)
25,205
(8281)
Cmin
(pg/mL)
NA
NA
NA
43.3
(15.8)
185
(53.5)
33.2
(9.69)
330
(139)
4566
(1581)
890
(300)
AUC0–24
M/P
NA
1.94
(0.459)
0.195
(0.0789)
NA
3.90
(1.14)
0.628
(0.223)
NA
12.9
(3.64)
2.57
(0.723)
% Active
AUC0–24a
28.0
(5.69)
52.6
(9.25)
5.20
(1.75)
16.0
(3.55)
58.8
(4.20)
9.39
(1.52)
6.06
(1.54)
73.1
(3.10)
14.7
(1.98)
Data are presented as mean (standard deviation), except for Tmax, which is presented as median (minimum–maximum)
AUC0–24 area under the concentration–time curve from 0 to 24 h postdose, Cmax maximum observed concentration over the dosing interval, Cmin minimum observed concentration over the dosing interval, M/P metabolite to parent ratio, NA not applicable, PK pharmacokinetic, Tmax time to Cmax
aActive AUC0–24 includes ozanimod, CC112273, CC1084037, RP101988, RP101442, and RP112289
×
Scatter plots with linear regression lines to illustrate the relationship of exposure (Cmax and AUC0–24) between the two major interconverting metabolites CC112273 and CC1084037 are shown in Fig. 2. On day 28, the coefficient of determination (R2) for Cmax and AUC0–24 was 0.976 and 0.979, respectively. The Cmax and AUC0–24 for CC1084037 were approximately 20% of the Cmax and AUC0–24 for CC112273.
Fig. 2
Relationship of CC112273 and CC1084037 Cmax and AUC0–24 on day 28. AUC0–24 area under the concentration–time curve from 0 to 24 h postdose, Cmax maximum observed concentration
×
Effect of Ozanimod on PSE-Induced Pressor Response
Table 2 shows the maximum time-matched change from baseline in BP and statistical analysis of the effect of ozanimod on PSE-induced BP response. PSE co-administered with placebo resulted in a mean maximum time-matched change in SBP from baseline of 14.64 mmHg. PSE co-administered with ozanimod resulted in a mean maximum time-matched change in SBP from baseline of 15.50 mmHg. The maximum time-matched change from baseline for DBP in the ozanimod + PSE group (9.63 mmHg) was also similar to the placebo + PSE group (8.96 mmHg). The LSM difference between ozanimod + PSE and placebo + PSE groups was 0.86 mmHg for SBP with a 90% CI of − 1.81, 3.53 mmHg and 0.64 mmHg for DBP with a 90% CI of − 1.23, 2.51 mmHg.
Table 2
Effect of ozanimod on PSE-induced pressor response: maximum time-matched change from baseline
Cardiovascular parameters (units)
Mean (SD)
LS means
LS means difference
(Test minus reference)
90% CI for LS means difference
(Test minus reference)
Ozanimod + PSE
(Test)
N = 28
Placebo + PSE (Reference)
N = 24
Ozanimod + PSE
(Test)
N = 28
Placebo + PSE (Reference)
N = 24
SBP (mmHg)
15.50 (5.418)
14.64 (5.952)
15.49
14.63
0.86
− 1.81, 3.53
DBP (mmHg)
9.63 (3.683)
8.96 (4.444)
9.74
9.10
0.64
− 1.23, 2.51
CI confidence interval, DBP diastolic blood pressure, LS least square, N number of subjects, PSE pseudoephedrine, SBP systolic blood pressure, SD standard deviation
Effect of Ozanimod on PSE PK
Mean (SD) plasma concentration–time profiles for a single dose of PSE when co-administered with ozanimod or placebo were superimposable (Fig. 3). Ozanimod had no effect on the exposure of PSE, as demonstrated by the geometric LSM ratios and associated 90% CIs for Cmax (0.98, 0.92–1.04) and AUC0–24 (1.03, 0.94–1.13) (Table 3).
Fig. 3
Mean (SD) PSE plasma concentration–time profiles for a single dose of PSE 60 mg when co-administered with ozanimod or placebo. PSE pseudoephedrine
Table 3
Statistical analysis to assess the effect of ozanimod on a single-dose exposure of PSE 60 mg
PSE primary PK endpoint (units)
Geometric LS means
Ratio of geometric LS means (test to reference)
90% CI for ratio of geometric LS means
Ozanimod + PSE (test)
N = 28
Placebo + PSE (reference)
N = 24
Cmax (ng/mL)
256
262
0.979
0.922, 1.040
AUC0–24 (ng × h/mL)
2450
2383
1.028
0.935, 1.130
AUC0–24 area under the concentration–time curve from time 0 to 24 h postdose, CI confidence interval, Cmax maximum observed plasma concentration, LS least square, PK pharmacokinetic, PSE pseudoephedrine
×
Effect of Ozanimod on Platelet MAO-B Activity
There was no relationship between the change from baseline in platelet MAO-B activity and the range of observed plasma CC112273 concentrations that encompassed the mean steady state Cmax (Cmax,ss) for CC112273 of 6980 µg/mL for ozanimod 0.92 mg QD in patients with MS [13] (Fig. 4a). The slope of the regression line was close to 0 with the 95% CI including 0. Similarly, there was no relationship between the change from baseline in platelet MAO-B activity and plasma CC1084037 concentrations with the slope of the regression line being close to 0 and the 95% CI including 0 (Fig. 4b).
Fig. 4
Change from baseline in platelet’s MAO-B activity versus plasma concentration for CC112273 (a) or CC1084037 (b). CI confidence interval, MAO monoamine oxidase
×
Safety
No serious AEs were reported. Overall, 34 subjects (63.0%) had at least one treatment-emergent AE (TEAE). In the placebo group, 14 subjects (53.8%) had at least one TEAE before PSE was co-administered and two subjects (8.3%) after PSE was co-administered. In the ozanimod group, 19 subjects (67.9%) had at least one TEAE before PSE was co-administered and four subjects (14.3%) after PSE was co-administered. The most frequently reported TEAEs in two or more subjects in the placebo treatment group were headache, nausea, vomiting, dizziness, constipation, and musculoskeletal pain. The most frequently reported TEAEs reported in two or more subjects in the ozanimod treatment group were headache, nausea, vomiting, constipation, rhinitis allergic, diarrhea, and anemia. The majority of all TEAEs were mild in severity. One subject in the placebo group completed dosing up to day 15 and was discontinued because of abnormal liver function enzymes (alanine aminotransferase [ALT] three times the upper limit of normal [ULN] and gamma glutamyl transferase four times the ULN). One subject in the placebo group was discontinued from the study prior to dosing on day 1 because of an AE of headache. There were no clinically significant changes in vital signs, and no bradycardia or ECG abnormalities. No notable trends for clinical laboratory test results were observed. For examples, the mean (SD) ALT at baseline for placebo and ozanimod groups were 19.8 (11.2) U/L and 16.9 (8.83) U/L, respectively, and at 7 days after the last dose were 19.5 (10.2) U/L and 21.7 (9.53) U/L, respectively. The mean (SD) blood urea nitrogen values at baseline for placebo and ozanimod groups were 4.32 (0.972) mmol/L and 4.47 (1.23) mmol/L, respectively, and at 7 days after the last dose were 4.43 (1.38) mmol/L and 4.99 (1.66) mmol/L, respectively.
Discussion
Ozanimod is approved in the United States for the treatment of adults with relapsing forms of MS and in the European Union for the treatment of adults with relapsing-remitting MS. The recommended maintenance dosage of of ozanimod is 0.92 mg orally QD. Ozanimod may cause a transient decrease in heart rate on dosing initiation. However, this negative chronotropic effect may attenuate over time, secondary to S1P1 receptor desensitization, internalization, and degradation on atrial myocytes [14]. An up-titration schedule of ozanimod 0.23 mg QD for 4 days followed by doses of 0.46 mg QD for 3 days prior to the maintenance dose of 0.92 mg attenuates the magnitude of heart rate reduction. To evaluate the potential effect of ozanimod and its major active metabolites on PSE and MAO-B activity, the systemic exposure of CC112273 and CC1084037 should be similar to the obtained exposure at steady state associated with the therapeutic dose of ozanimod 0.92 mg QD in patients with MS. Considering the t1/2 of approximately 10 days for both major active metabolites, the required ozanimod dosing would be approximately five times the mean t1/2 or 50 days, which is not reasonable for healthy subjects. Therefore, ozanimod was titrated to 1.84 mg over 30 days (0.23 mg QD on days 1–4, 0.46 mg QD on days 5–7, 0.92 mg QD on days 8–10, and 1.84 mg QD on days 11–30) in this study to achieve the observed steady-state exposure of CC112273 and CC1084037 in patients with MS receiving the maintenance dose of 0.92 mg QD. A two-way crossover design for drug interaction evaluation is preferred over parallel-study designs. However, as a result of the long t1/2 of the major active metabolites (approx. 10 days), a crossover study design is not feasible and therefore a parallel-group design was used.
In this study, ozanimod, CC112273, and CC1084037 each represented approximately 28%, 53%, and 5%, respectively, of circulating total active drug exposure upon initiation of ozanimod 0.23 mg on day 1. With repeated dosing, the percentage of total active exposure for CC112273 and CC1084037 continued to increase, which was anticipated because of their long t1/2. On day 28, approximately 94% of circulating total active drug exposure is represented by ozanimod (6%), CC112273 (73%), and CC1084037 (15%). The mean Cmax for the major active metabolites on day 28 observed in this study was also similar to the mean steady-state Cmax in the patients with MS (data on file). Consistent with the single-dose PK results [4], exposures of CC112273 and CC1084037 were highly correlated following multiple dosing.
MAO inhibitors for the treatment of depression (e.g., phenelzine, tranylcypromine) or Parkinson’s disease (e.g., safinamide, rasagiline, selegiline) can interact with sympathomimetic drugs, resulting in sudden BP elevations, which may lead to hypertensive crises. MAO inhibitors are also known to significantly inhibit platelet MAO-B activity [15‐17]. Since the major active metabolites of ozanimod selectively inhibited MAO-B in vitro, we conducted the clinical study to evaluate the effect of ozanimod treatment on the PSE-induced pressor response. Results showed that ozanimod multiple dosing to achieve steady-state exposure for the major active metabolites associated with the 0.92 mg QD in patients with MS did not potentiate the effect of PSE on BP. The lack of ozanimod treatment effect on the pressor response of PSE could be attributed to the lack of CC112273 inhibitory effect on platelet MAO-B activity.
Certain antibiotics such as linezolid and tedizolid also inhibit MAO in vitro. While linezolid increased the BP response to PSE, tedizolid showed no meaningful interaction with PSE [18, 19]. One of the potential explanations for the difference in the interaction was the relationship between free-drug concentrations in plasma and the IC50 for MAO inhibition. The tedizolid estimated free Cmax was about 4.3-fold and 6.7-fold lower than the IC50 of MAO-A and MAO-B activity, respectively, while the linezolid free Cmax was similar to (0.94-fold) and 21-fold higher than IC50 for MAO-A and MAO-B, respectively [19]. On the basis of the plasma protein binding of 99.8% and 99.3% for CC112273 and CC1084037, respectively (data on file), free CC112273 and CC1084037 Cmax in this study (which are also similar to Cmax,ss in patients with MS receiving ozanimod maintenance dose of 0.92 mg QD) were less than 1% and 0.001% of the IC50 for MAO-A and MAO-B activity, respectively. These observations are also consistent with the lack of in vivo effect on human platelet MAO-B activity. However, the clinical relevance of the relationship between free-drug concentrations and MAO activity needs further confirmation.
Conclusions
Following multiple dosing in healthy adults, approximately 94% of circulating total active drug exposure is represented by ozanimod (6%), CC112273 (73%), and CC1084037 (15%). Exposures of the major and interconverting metabolites CC112273 and CC1084037 were highly correlated. While CC112273 and CC1084037 selectively inhibited MAO-B in vitro, these major active metabolites did not inhibit human platelet MAO-B activity in vivo. Concomitant administration of ozanimod with PSE, a sympathomimetic agent, did not potentiate the effects on BP.
Acknowledgements
We thank the participants of this study.
Funding
This study was sponsored by Celgene Corporation. The Rapid Service and Open Access Fees were funded by Bristol-Myers Squibb Company.
Editorial Assistance
Support for third-party editorial assistance for this manuscript was provided by Lorraine Sweeney of Peloton Advantage, LLC, an OPEN Health company, and was funded by Bristol-Myers Squibb Company.
Authorship
All named authors meet the International Committee of Medical Journal Editors criteria for authorship for this article, take responsibility for the integrity of the work as a whole, and have given their approval for this version to be published.
Disclosures
Jonathan Q Tran is a former employee of Bristol-Myers Squibb Company and a current employee of Mirati Therapeutics. Peijin Zhang, Atalanta Ghosh, Mary Syto, Xiaomin Wang, Sarah Harris, and Maria Palmisano are employees of Bristol-Myers Squibb Company. Susan Walker is an independent consultant (Apex Biostatistics).
Compliance with Ethics Guidelines
The study protocol and informed consent were reviewed and approved by an institutional review board (IntegReview, Austin, TX, USA). All subjects provided written informed consent before study entry. The study was conducted in accordance with the ethical principles of Good Clinical Practice and the Declaration of Helsinki.
Data Availability
Celgene, a Bristol-Myers Squibb Company, is committed to responsible and transparent sharing of clinical trial data with patients, healthcare practitioners, and independent researchers for the purpose of improving scientific and medical knowledge as well as fostering innovative treatment approaches. Data requests may be submitted to Celgene, a Bristol-Myers Squibb Company, at https://vivli.org/ourmember/celgene/ and must include a description of the research proposal. The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
Open Access This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License, which permits any non-commercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc/4.0/.
Mit e.Med Innere Medizin erhalten Sie Zugang zu CME-Fortbildungen des Fachgebietes Innere Medizin, den Premium-Inhalten der internistischen Fachzeitschriften, inklusive einer gedruckten internistischen Zeitschrift Ihrer Wahl.
Mit e.Med Allgemeinmedizin erhalten Sie Zugang zu allen CME-Fortbildungen und Premium-Inhalten der allgemeinmedizinischen Zeitschriften, inklusive einer gedruckten Allgemeinmedizin-Zeitschrift Ihrer Wahl.
Multiple-Dose Pharmacokinetics of Ozanimod and its Major Active Metabolites and the Pharmacodynamic and Pharmacokinetic Interactions with Pseudoephedrine, a Sympathomimetic Agent, in Healthy Subjects
verfasst von
Jonathan Q. Tran Peijin Zhang Susan Walker Atalanta Ghosh Mary Syto Xiaomin Wang Sarah Harris Maria Palmisano
Ob bei einer Notfalloperation nach Schenkelhalsfraktur eine Hemiarthroplastik oder eine totale Endoprothese (TEP) eingebaut wird, sollte nicht allein vom Alter der Patientinnen und Patienten abhängen. Auch über 90-Jährige können von der TEP profitieren.
Wenn unter einer medikamentösen Hochdrucktherapie der diastolische Blutdruck in den Keller geht, steigt das Risiko für schwere kardiovaskuläre Ereignisse: Darauf deutet eine Sekundäranalyse der SPRINT-Studie hin.
Insektenstiche sind bei Erwachsenen die häufigsten Auslöser einer Anaphylaxie. Einen wirksamen Schutz vor schweren anaphylaktischen Reaktionen bietet die allergenspezifische Immuntherapie. Jedoch kommt sie noch viel zu selten zum Einsatz.
Beginnen ältere Männer im Pflegeheim eine Antihypertensiva-Therapie, dann ist die Frakturrate in den folgenden 30 Tagen mehr als verdoppelt. Besonders häufig stürzen Demenzkranke und Männer, die erstmals Blutdrucksenker nehmen. Dafür spricht eine Analyse unter US-Veteranen.
Update Innere Medizin
Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.
