Musashi-2, a novel oncoprotein promoting cervical cancer cell growth and invasion, is negatively regulated by p53-induced miR-143 and miR-107 activation

Human CC cell lines HeLa and SiHa (American Type Culture Collection, Manassas, VA) and immortalized normal cervical epithelial squamous cell line H8 (Chinese Academy of Sciences Cell Bank, Shanghai, China) were cultured in DMEM/F12 medium (Invitrogen) supplemented with 10% fetal bovine serum (Invitrogen). These cell lines were routinely tested by PCR for mycoplasma contamination by using the following primers: Myco_fw1: 5′-ACACCATGGGAGCTGGTAAT-3′, Myco_rev1: 5′-CTTCATCGACTTTCAGACCCAAGGCA-3′. MiRNA mimic and miRNA inhibitor for miR-143 or miR-107, and respective controls were obtained from Ambion (Austin, TX). MSI-2 small interfering RNA (siRNA) and control siRNA were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). The cDNA plasmid encoding human MSI-2, c-FOS and p53 were purchased from OriGene (Rockvill, MD). Transient transfection experiments were performed using Lipofectamine 3000 (Invitrogen, Carlsbad, CA) according to the manufacturer’s protocol. Mithramycin A was obtained from (Sigma-Aldrich, St. Louis, MO).

The total RNA was isolated using TRIzol (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions. 100 ng of total RNA from each sample was subjected to first-strand cDNA synthesis using PrimeScript RT reagent kit (Takara, Otsu, Japan). For mature miRNA quantitation, miR-143 or miR-107 expression was determined using the NCode miRNA qRT-PCR analysis (Invitrogen, Carlsbad, CA) following manufacturer-recommended protocols. Forward primer is the exact sequence of the mature miR-143 or miR-107. The primers used in the PCR reaction were purchased from Applied Biosystems (Carlsbad, CA). Real-time PCR was conducted by using ABI PRISM 7000 Sequence Detection System (Applied Biosystems). GAPDH or U6 was used as internal control for the normalization of mRNA or miRNA, respectively. qRT-PCRs were performed in triplicate, and data was presented as fold change from control.

Total proteins were extracted from cell lines 48 h after transfections using M-PER reagent (Pierce, Rockford, IL). The protein concentration was determined using the Bio-Rad protein assay system (Bio-Rad, Hercules, CA). Total protein lysates (30 μg) were fractionated using SDS-PAGE and transferred onto PVDF membranes. The following primary antibodies were used: anti-MSI-2 (Abcam, ab76148), anti-c-FOS (Abcam, ab156802), anti-PTEN (Abcam, ab32199), anti-p53 (Santa Cruz, sc-126), anti-p21 (Santa Cruz, sc-6246) and anti-GAPDH (Santa Cruz, sc-47,778). Blots were developed using horseradish peroxidase-conjugated secondary antibody and the enhanced chemiluminescence detection system (Amersham, Little Chalfont, UK). Primary and secondary antibodies were used at 1:1000 and 1:5000 dilutions, respectively.

The invasive ability of CC cells was tested using BioCoat Matrigel Invasion Chamber (BD Biosciences, San Jose, CA) as described previously [27, 28]. Briefly, transfected CC cells with serum-free medium were seeded into the upper chamber of the system. Bottom wells in the system were filled with complete medium. After 24 h of incubation, the cells in the upper chamber were gently removed with a cotton swab, and the cells that invaded through Matrigel matrix membrane were stained with Giemsa. Then, the number of invaded cells were counted under a microscope.

Cell proliferation was measured by using cell counting kit-8 (CCK-8) following manufacturer’s instruction (Dojindo, Kumamoto, Japan). CC cells were plated in 96-well plates at a density of 1 × 10⁴ cells per well and subjected to the indicated transfection or treatment. After 72 h of incubation, 10 μl of CCK-8 solution was added into each well and the plates were incubated for additional 4 h at 37 °C. The absorbance at 450 nm was determined using a microplate reader. The experiment was performed in triplicate wells and repeated three times.

Single-cell suspensions were suspended at a density of 5000 cells/ml in serum-free DMEM/F12 medium containing N2 plus media supplement (Invitrogen, CA), epidermal growth factor (20 ng/ml), basic fibroblast growth factor (20 ng/ml) and heparin (4 mg/ml) in 6-well Ultra-Low attachment plates (Corning, NY). Fresh medium was added to each well every 3 days. The suspension cultures were continued for 14 days, and then the number of spheres larger than 50 μm was counted.

RNA immunoprecipitation (RNA-IP) was performed using Magna RIP RNA Binding Protein Immunoprecipitation Kit (Millipore, Billerica, MA) according with manufacturer’s instructions. In brief, cells were washed with cold phosphate-buffered saline and lysed with RIP lysis buffer provided in the kit. Next, 5 μg of anti-MSI-2 antibody (part of the kit 03–115; Millipore) or anti-IgG control antibody (Millipore) was incubated with magnetic beads, and used to immunoprecipitate endogenous MSI-2-RNA complexes. After the immunoprecipitated complexes were washed, they were treated with proteinase K. RNA extraction was performed by the phenol–chloroform method, and purified RNA was used for qRT–PCR to check RNA binding with MSI-2 protein. Results are presented relative to IgG immunoprecipitation, set as 1.

Human c-FOS or MSI-2 3′-UTR luciferase-reporter vector was obtained from OriGene Technologies (Rockville, MD). The mutant c-FOS 3′-UTR vector containing the mutation in predicted MSI-2-binding sequence (TAGTA to AAAAA), or the mutant MSI-2 3′-UTR vector carrying mutations at putative miR-143 or miR-107-binding site, were generated using a QuickChange site-directed mutagenesis kit (Stratagene, CA). CC cells were transfected with firefly luciferase reporter vector, Renilla reporter plasmid pRL-CMV, together with MSI-2 siRNA, miRNA mimic, anti-miRNA inhibitor or their negative controls. 24 h after transfection luciferase activity was assessed with the Dual-Luciferase Reporter Assay system (Promega). The ratio of firefly/Renilla luciferase activity was determined and reported as relative luciferase activity. The relative luciferase activity in cells transfected with control siRNA, control miRNA mimic or control miRNA inhibitor was set to 1.

Following an institutional review board-approved protocol, primary CC specimens (n = 58) and normal tumor-adjacent cervical tissues (n = 58) were collected at the Cancer Center, Sun Yat-Sen University, China. All samples were obtained at primary resection, and none of the patients had been subjected to chemotherapy or radiation therapy before resection. Samples were snap-frozen and stored in liquid nitrogen until RNA extraction.

The results are presented as the mean ± SEMs from at least three independent replicates. For experiments in vitro, 2-tailed Student’s t-test or 1-way ANOVA was used. The difference in mRNA or miRNA expression between CC and normal cervical tissues were evaluated using the nonparametric Mann-Whitney U-Test. P-values <0.05 were regarded as significant.

Using qRT-PCR analysis, we observed a significantly increased expression of MSI-2 as well as two known oncogenes (EZH2 and iASPP) [9, 28] in CC tissues compared with normal cervical tissues (Fig. 1a). Consistently, HeLa and SiHa human CC cells showed abundant MSI-2 protein expression, whereas this protein had weak expression in immortalized human cervix epithelial cell line H8 (Fig. 1b). By using the BioExpress database [29], we analyzed the TCGA pan-cancer data to evaluate MSI-2 mRNA expression in a variety of human normal and cancerous tissues based on the Affymetrix GeneChip platform. Compared to normal tissues, MSI-2 expression was elevated in numerous human cancers including cervical, prostate, bladder, rectum, endometrial, liver, pancreatic, lung, esophageal, stomach and colon cancer (Fig. 1c). Moreover, we used the MethHC database that provided gene expression patterns from TCGA across 18 major human cancers [30] to compare the mRNA expression of MSI-2 in normal and tumor tissues. The MSI-2 mRNA expression was highly overexpressed in a variety of cancer types, including cervical, breast, head and neck, kidney papillary, lung, sarcoma, skin melanoma, and thyroid cancer as compared to the corresponding normal tissues (Fig. 1d). Furthermore, we retrieved cancer data sets from the OncoLnc web tool [31] that links TCGA survival data of CC patients to mRNAs to determine the prognostic value of MSI-2 in human CC. 264 CC patients were divided into high expression group and low expression group by the median. Higher expression of MSI-2 was significantly correlated with worse disease prognosis (Fig. 1e). We also defined the relationship between MSI-2 mRNA expression and the overall survival in human lung, pancreatic, adrenal cancer and glioblastoma patient data sets using the PROGgene web site [32]. For these tumor types, high expression of MSI-2 mRNA was significantly associated with poor prognosis (Fig. 1f). Taken together, these results indicate that MSI-2 may function as a tumor promoter in CC and possibly other tumor types.

To enable functional characterization of MSI-2, we transiently transfected HeLa cells that express relatively higher levels of MSI-2 protein with either human MSI-2 siRNA or control siRNA. In addition, SiHa cells that show relatively lower levels of MSI-2 were transfected with control plasmid alone or with expression plasmid coding for MSI-2. Downregulation or up-regulation of MSI-2 protein was confirmed using Western blotting analysis (Fig. 2a). We used Matrigel invasion chamber assay to examine the invasive potential of CC cells transfected with specific MSI-2 siRNA or MSI-2 expression plasmid. In contrast to HeLa cells transfected with control siRNA, MSI-2 siRNA-transfected HeLa cells showed a significantly lower level of penetration (Fig. 2b). However, the number of cells passed across the Matrigel layer was significantly increased in SiHa cells transduced with MSI-2 expression plasmid, compared to control plasmid (Fig. 2c). These results indicated that overexpression of MSI-2 can enhance in vitro tumor invasion for CC cells.

To evaluate the effects of MSI-2 modulation on CC cell proliferation, we performed CCK-8 assay, and found that MSI-2-depleted HeLa cells had significantly reduced growth rates, and MSI-2 overexpression caused a significant increase in the cell growth rate (Fig. 2d). The contribution of MSI-2 to cancer stemness was then assessed via sphere formation assay. The number of sphere was significantly decreased in MSI-2-depleted HeLa cells, buy significantly increased in SiHa cells overexpressing MSI-2 (Fig. 2e and f), indicating that MSI-2 has a critical function in CC cell growth.

To elucidate the molecular basis whereby MSI-2 promotes the malignant behaviors of CC cells, we examined the mRNA expression of several metastasis-related genes (SNAIL, Vimentin and E-cadherin) and cancer stem cell marker CD44 in MSI-2 overexpression cells or knockdown cells. The results showed that the levels of SNAIL, Vimentin and CD44 were decreased in MSI-2 knockdown HeLa cells. Conversely, inhibition of MSI-2 induced the mRNA levels of E-cadherin (Fig. 2g). The opposite results were found in SiHa cells when MSI-2 was overexpressed (Fig. 2g). These results point to the crucial role of MSI-2 activation in accelerating CC cell invasion and proliferation.

In pancreatic cancer cells, MSI-2 loss led to down-regulation of many key proto-oncogenes including c-FOS [33]. Because c-FOS plays a key role in cancer progression of various cancer types, including CC [7, 34], we examined whether its expression is regulated by MSI-2 in CC cells. Immunoblotting analysis showed that the levels of c-FOS significantly declined in MSI-2-depleted HeLa cells. However, c-FOS protein expression was increased following MSI-2 overexpression in SiHa cells (Fig. 3a). As a control, the expression of tumor suppressor PTEN, a downstream target of MSI-2 [35], was examined. We found that knockdown of MSI-2 increased PTEN levels, whereas overexpression of MSI-2 reduced its expression (Fig. 3a). qRT-PCR analysis suggested that the levels of c-FOS mRNA was not affected following either knockdown or overexpression of MSI-2 (Fig. 3b), suggesting that MSI-2 may induce c-FOS protein levels by controlling c-FOS mRNA translation.

We identified one MSI-2 binding site (TAGTA) in the c-FOS 3′-UTR region (Fig. 3c) [36, 37]. Using RNA-IP, we assessed whether MSI-2 protein binds to c-FOS mRNA in CC cells. The results showed that the mRNA of c-FOS was highly enriched in MSI-2-antibody precipitated RNA fraction in HeLa and SiHa cells (Fig. 3d). To examine the direct interaction between of MSI-2 protein and c-FOS mRNA, c-FOS 3′-UTR reporter construct carrying the wild-type or the mutant MSI-2 binding site was tested. Compared with the control siRNA, we detect a significant decrease in luciferase activity of wild-type c-FOS 3′-UTR reporter when we transfected MSI-2 siRNA in HeLa and SiHa cells (Fig. 3e). However, down-regulation of MSI-2 had no repressive effect on the mutant c-FOS 3′-UTR construct (Fig. 3e). In total, our experiments indicated that MSI-2 facilitates c-FOS mRNA translation, via direct binding to its 3′-UTR. We further investigated whether c-FOS is indeed involved in MSI-2-mediated tumor promotion in CC. A plasmid expressing c-FOS cDNA was co-transfected with MSI-2 siRNA in HeLa cells (Fig. 3f). Importantly, overexpression of c-FOS partially rescued MSI-2 siRNA-inhibited cell invasion, proliferation and sphere formation (Fig. 3g–i). These results support that MSI-2 promotes CC cell growth, invasiveness and sphere formation by facilitating c-FOS mRNA translation.

Analysis using publicly available algorithms predicted that miR-143 and miR-107 might be potential regulators of MSI-2 (Fig. 4a). Our qRT-PCR analysis showed that miR-143 and miR-107 expression were significantly reduced in HeLa and SiHa cells compared with normal H8 cells (Fig. 4b). Meanwhile, luciferase assays showed overexpressing miR-143 or miR-107 via miRNA mimic attenuated, whereas silencing of these two miRNAs via miRNA inhibitor elevated the reporter activities driven by the 3′-UTR of MSI-2 transcript (Fig. 4c and d). As expected, the observed luciferase activity changes were abrogated when the miR-143 or miR-107 binding site in MSI-2 3′-UTR was mutated (Fig. 4c and d). Consistently, Western blotting analysis revealed that the expression of MSI-2 as well as c-FOS was dramatically decreased in miR-143 or miR-107 mimic-transfected HeLa cells (Fig. 4e), but increased in miR-143 or miR-107-silenced SiHa cells (Fig. 4f). Moreover, overexpression of MSI-2 significantly reversed miR-143 or miR-107-mediated inhibition of invasion, proliferation and sphere formation of HeLa cells (Fig. 4g–i). These results suggest that miR-143 and miR-107 are direct suppressors of MSI-2 and inhibit CC cell invasion and proliferation.

To address the relevance of miR-143 or miR-107 expression to human CC, we examined the levels of these miRNAs in primary CCs using qRT-PCR analysis. Both miR-143 and miR-107 were markedly downregulated in cancer tissues as compared with adjacent normal cervical tissues (Fig. 5a). The qRT-PCR analysis also suggested that miR-143 and miR-107 expression levels were significantly lower in CC patients with advanced stage disease (Fig. 5b), and in CC patients with lymph node metastasis (Fig. 5c). Notably, we found that patients with lower expression of miR-143 or miR-107 had a shorter survival time (Fig. 5d). Taken together, these results suggest that reduced miR-143 and miR-107 expression correlates with CC progression.

miR-143 and miR-107 were shown to be induced by tumor suppressor p53 at post-transcriptional and transcriptional levels, respectively [11, 12]. Interestingly, treatment with Mithramycin A led to activation of p53, and can trigger senescence and apoptosis of malignant pleural mesothelioma cells [21]. Therefore, we postulated that the addition of Mithramycin A could increase miR-143 or miR-107 expression through p53 induction, thereby resulting in the downregulation of MSI-2 and the suppression of CC cell invasion and proliferation. Western blot analysis showed that Mithramycin A upregulated the protein expression of p53, p21 (a downstream effector of p53), but downregulated MSI-2 expression in both CC cell lines in a dose-dependent manner (Fig. 6a). Consistently, treatment with Mithramycin A significantly restored the expression of miR-143 and miR-107 in CC cells (Fig. 6b). To understand whether p53 regulates the expression MSI-2, miR-143 and miR-107 in CC cells used in this study, we investigated the impact of transient p53 overexpression on MSI-2 expression. Compared to cells transfected with a control plasmid, HeLa and SiHa cells transfected with a plasmid expressing p53 cDNA showed reduced protein levels of MSI-2 (Fig. 6c), and exhibited increased expression of miR-143 and miR-107 (Fig. 6d). Finally, cell functional studies demonstrated that Mithramycin A-treated CC cells displayed a reduced capacity of invasion, proliferation and sphere formation than DMSO-treated control cells (Fig. 6e). Collectively, these results suggest that upregulation of miR-143 and miR-107 by Mithramycin A via p53 activation decreases MSI-2 levels and suppresses invasion and proliferation of CC cells.