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Journal of Endocrinological Investigation

Erschienen in:

01.04.2020 | Original Article

Nesfatin-1 ameliorates type-2 diabetes-associated reproductive dysfunction in male mice

verfasst von: A. Ranjan, M. Choubey, T. Yada, A. Krishna

Erschienen in: Journal of Endocrinological Investigation | Ausgabe 4/2020

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Abstract

Purpose

The present study was aimed to demonstrate the recuperative effect of nesfatin-1 on testicular dysfunction in the high-fat diet (HFD)/streptozotocin (STZ)-induced type-2 diabetes mellitus (T2DM) mice.

Method and results

Three experimental groups were formed: (1) vehicle control (VC), (2) T2DM mice, (3) T2DM + nesf-1. The mice with blood glucose level higher than 300 mg/dL following HFD and a single dose of STZ were used for the experiment. The T2DM mice showed increases in body mass, blood glucose and insulin levels, reductions in spermatogenesis and steroidogenesis, production of antioxidative enzymes, and disturbed lipid profile. These alterations were all ameliorated by administration of nesfatin-1 at 20 μg/Kg BW for 15 days. Nesfatin-1 treatment also increased the production of testosterone (T), improved insulin sensitivity, and effectively ameliorated the testicular aberrations, and increased spermatogenesis and steroidogenesis. In addition, nesfatin-1 treatment upregulated the PCNA and Bcl2 expression and inhibited the caspase-3 and prohibitin expression in T2DM mice. Nesfatin-1 increased insulin receptor (IR) and GLUT8 expressions, and lactate production, the changes that further substantiate the increase of energy influx to the testis.

Conclusion

Altogether, the results suggest the ameliorative effect of nesfatin-1 against T2DM-associated testicular dysfunctions and improved insulin sensitivity along with promoting T production and fertility in T2DM mice.

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Titel: Nesfatin-1 ameliorates type-2 diabetes-associated reproductive dysfunction in male mice
verfasst von: A. Ranjan
M. Choubey
T. Yada
A. Krishna
Publikationsdatum: 01.04.2020
Verlag: Springer International Publishing
Erschienen in: Journal of Endocrinological Investigation / Ausgabe 4/2020
Elektronische ISSN: 1720-8386
DOI: https://doi.org/10.1007/s40618-019-01136-0

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