Background
Methods
Monoclonal antibodies and reagent
Serum samples
Luminex assay
Confirmation of bead coupling
Cut-off index
Results
Monoclonal antibody-coupled beads confirmation
Specificity and sensitivity of Luminex assay detecting HPA antibodies
Specificity of Luminex assay detecting HLA antibody
Detecting HPA and HLA antibodies from clinical samples simultaneously
HPA-1 | HPA-2 | HPA-3 | HPA-4 | HPA-5 | HPA-15 | |
---|---|---|---|---|---|---|
G1 | aa | ab | ab | aa | aa | ab |
G2 | aa | ab | aa | aa | aa | bb |
G3 | aa | aa | bb | aa | aa | aa |
G4 | aa | aa | ab | aa | ab | bb |
Sample | Platelet | MFI | Luminex results | MAIPA results | |||
---|---|---|---|---|---|---|---|
GPIa/IIa (20) | GPIba/IX (36) | HLA (50) | GPIIb/IIIa (66) | ||||
S2 | G1 | 83.59 | 414.37 | 1399 | 72.18 | Anti-HLA + anti-HPA-2b | Anti-HLA + anti-HPA-2b |
G2 | 89.70 | 468.21 | 438.5 | 108.04 | |||
G3 | 52.42 | 71.95 | 3341 | 86.68 | |||
G4 | 46.49 | 69.51 | 1442 | 58.83 | |||
S3 | G1 | 71.05 | 36.19 | 1390 | 70.60 | Anti-HLA + anti-HPA-5b | Anti-HLA + anti-HPA-5b |
G2 | 57.32 | 68.15 | 2469 | 74.08 | |||
G3 | 83.57 | 43.42 | 2776.5 | 111.12 | |||
G4 | 941.90 | 71.25 | 1395 | 71.52 | |||
T5 | G1 | 56.06 | 21.63 | 45.19 | 3430.30 | Anti-HPA-1a | Anti-HPA-1a |
G2 | 59.48 | 43.65 | 116.05 | 1053.89 | |||
G3 | 69.32 | 53.31 | 71.13 | 1318.20 | |||
G4 | 33.28 | 36.64 | 45.39 | 3997.51 | |||
S7 | G1 | 1402.07 | 39.17 | 109.89 | 1355.66 | Anti-HPA-5a + anti-HPA-1a | Anti-HPA-5a + anti-HPA-1a |
G2 | 1410.06 | 34.86 | 111.47 | 1406.88 | |||
G3 | 1114.12 | 40.45 | 33.44 | 1297.48 | |||
G4 | 1498.50 | 79.59 | 106.75 | 1810.53 | |||
T8 | G1 | 33.77 | 41.91 | 103.64 | 1347.68 | Anti-HLA + anti-HPA-3a | Anti-HLA + anti-HPA-3a |
G2 | 52.63 | 90.44 | 3675.49 | 1764.84 | |||
G3 | 52.21 | 47.67 | 2065.35 | 92.82 | |||
G4 | 51.73 | 37.52 | 1078.52 | 1153.10 | |||
S9 | G1 | 76.06 | 79.44 | 559.08 | 876.67 | Anti-HLA + anti-HPA-3b | Anti-HLA + anti-HPA-3b |
G2 | 76.41 | 69.50 | 5511.44 | 107.48 | |||
G3 | 61.12 | 79.00 | 1961.17 | 785.68 | |||
G4 | 69.75 | 83.96 | 3080.16 | 976.73 | |||
T10 | G1 | 69.80 | 23.18 | 71.49 | 77.42 | Anti-HPA-5b | Anti-HPA-5b |
G2 | 74.64 | 38.32 | 69.92 | 44.98 | |||
G3 | 70.63 | 31.47 | 69.48 | 97.10 | |||
G4 | 1979.27 | 80.06 | 78.05 | 54.36 | |||
S11 | G1 | 50.80 | 47.89 | 28.64 | 423.74 | Anti-HPA-1a | Anti-HPA-1a |
G2 | 56.66 | 71.67 | 37.95 | 1022.23 | |||
G3 | 93.93 | 80.44 | 64.35 | 1248.52 | |||
G4 | 43.16 | 35.45 | 72.41 | 1203.06 | |||
S12 | G1 | 35.40 | 22.88 | 3135.34 | 91.55 | Anti-HLA | Anti-HLA |
G2 | 41.92 | 74.05 | 14,619.87 | 130.06 | |||
G3 | 31.82 | 68.35 | 11,924.19 | 85.82 | |||
G4 | 54.81 | 29.88 | 9249.19 | 83.82 | |||
Cut-off | 132.29 | 85.19 | 131.97 | 163.46 | 132.29 |