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Tumor Biology

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01.10.2014 | Review

Novel methodologies in analysis of small molecule biomarkers and living cells

verfasst von: Yinan Chen, Zhenggang Zhu, Yingyan Yu

Erschienen in: Tumor Biology | Ausgabe 10/2014

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Abstract

Enzyme-linked immuno-sorbent assay (ELISA) is widely used for biomarker detection. A good biomarker can distinguish patients from healthy or benign diseases. However, the ELISA method is not suitable for small molecule or trace substance detection. Along with the development of new technologies, an increasing level of biomaterials, especially small molecules, will be identified as novel biomarkers. Quantitative immuno-PCR, chromatography–mass spectrometry, and nucleic acid aptamer are emerging methodologies for detection of small molecule biomarkers, even in living cells. In this review, we focus on these novel technologies and their potential for small molecule biomarkers and living cell analysis.

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Titel: Novel methodologies in analysis of small molecule biomarkers and living cells
verfasst von: Yinan Chen
Zhenggang Zhu
Yingyan Yu
Publikationsdatum: 01.10.2014
Verlag: Springer Netherlands
Erschienen in: Tumor Biology / Ausgabe 10/2014
Print ISSN: 1010-4283
Elektronische ISSN: 1423-0380
DOI: https://doi.org/10.1007/s13277-014-2439-2

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